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Expression of the mosquitocidal-protein genes ofBacillus thuringiensis subsp.israelensis and the herbicide-resistance genebar inSynechocystis PCC6803

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Abstract

CyanobacteriumSynechocystis PCC6803 was used as a model system for a prolonged delivery of insecticidal crystal proteins ofBacillus thuringiensis subsp.israelensis in the water surface. Thebt8 gene, encoding a 128 kDa (Bt8) mosquitocidal protein, and the28kd gene, encoding a 28 kDa cytotoxic protein, were integrated into the cyanobacterial chromosome. The genes were expressed under the control of thepsbA promoter, derived from the tobacco chloroplast genome. The Bt8 protein produced by the cyanobacterium was toxic to mosquito larvae. The28kd gene expression in the cyanobacterium was very low, partly owing to the low level of steady state mRNA. With the same system, it was demonstrated that the herbicide-resistance genebar could be used as a new selectable marker in cyanobacterial transformation experiments.

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  1. Plant Genetic Systems N.V., Gent, Belgium

    Wipa Chungjatupornchai

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  1. Wipa Chungjatupornchai
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Chungjatupornchai, W. Expression of the mosquitocidal-protein genes ofBacillus thuringiensis subsp.israelensis and the herbicide-resistance genebar inSynechocystis PCC6803. Current Microbiology 21, 283–288 (1990). https://doi.org/10.1007/BF02092092

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