Abstract
The root ofSalvia miltiorrhiza is a traditional Chinese medicinal plant used as an important drug to cure cardiovascular diseases. Research on the technology of induction, identification and chemical analysis of polypoid plants is reported. The obtained results indicated that basal MS media plus 10 ppm colchicine can induce polyploid mutants effectively. Tetraploid plants were transferred to the fields so that comparative experiment for further identification, observation and screening of 15 agronomic characteristics could be made. The major chemical compounds, tanshinones, in two tetraploid plants were higher than that in the control. An excellent plant 61-2-22 may develop into a new variety for large scale production.
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Abbreviations
- BA:
-
bezylaminopurine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- B5 :
-
Gamborg et al. (1968) medium
- HPLC:
-
high pressure liquid chromatography
- MS:
-
Murashige and Skoog (1962) medium
References
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Lewis WH (1980) Polyloidy: Biological Relevance. Plenum Press, New York (pp 180–182).
Murashige T & Skoog F (1962) A revised medium for rapid growth aand bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pei XS (1985) Polyploidy Induction and Breeding Shanghai Science and Technology Press (pp.95–98)
Rowson JM (1949) Increased alkaloid contents of induced polyploid ofDatura. Nature 154: 81–82
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Gao, S.L., Zhu, D.N., Cai, Z.H. et al. Autotetraploid plants from colchicine-treated bud culture ofSalvia miltiorrhiza Bge . Plant Cell Tiss Organ Cult 47, 73–77 (1996). https://doi.org/10.1007/BF02318968
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DOI: https://doi.org/10.1007/BF02318968