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Amination of Poly(ethylene-terephthalate) polymer surface for biochemical applications

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Plasmas and Polymers

Abstract

Amine functionalization of Poly(ethylene-terephthalate) (PET) films for covalent binding of peptides is described. Ammonia plasma treatments have been used to graft nitrogen-containing functional groups onto the PET surface. The samples were then analyzed by X-ray photoelectron spectroscopy (XPS) and a parametric study was performed to define the best plasma grafting conditions. For biological tests, samples were sterilized by steam autoclaving: this induces a four to fivefold loss of the nitrogen functional groups on the polymer surface. XPS does not differentiate easily between the various nitrogen groups present on the surface so it is difficult to estimate the amount of surface amine groups available for direct coupling of bioactive molecules (proteins, peptides, nucleic acids, ...). To obtain a direct measurement of the amines present, we assayed for cysteine fixation through its carboxylic group by detection of the thiolaminoacid by XPS. We obtained cysteine fixation, showing the presence of grafted primary amine functions on PET surface after ammonia plasma treatment. Radiochemical assays were also made to quantify the amount of amine groups on plasma treated PET. XPS, cysteine fixation and radiochemical assays all show the presence of amine functions on ammonia plasma treated PET.

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Authors and Affiliations

  1. LISE, Facultés Universitaires Notre-Dame de la Paix, 61 rue de Bruxelles, B5000, Namur, Belgium

    C. Girardeaux, N. Zammatteo, M. Art, B. Gillon, J. J. Pireaux & R. Caudano

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  1. C. Girardeaux
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  2. N. Zammatteo
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  3. M. Art
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  5. J. J. Pireaux
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Girardeaux, C., Zammatteo, N., Art, M. et al. Amination of Poly(ethylene-terephthalate) polymer surface for biochemical applications. Plasma Pol 1, 327–346 (1996). https://doi.org/10.1007/BF02532829

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  • DOI: https://doi.org/10.1007/BF02532829

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