Abstract
Animal glue (collagen proteins) degradation was studied in water and in a simulated copper electrolyte (150 g/L H2SO4, 46 g/L Cu2+ as CuSO4) by size-exclusion chromatography. The rate of degradation was relatively slow in pure aqueous solutions, and depending on the temperature and glue concentration, some association to larger molar mass species was observed. For simulated electrolyte in a temperature range of 42 °C to 70 °C and a glue concentration range of 100 to 3000 mg/L, the degradation rate constant was described with the following relation:k′ = 1.5· 107exp (-9951/T), min-1
The degradation rate was zero order with respect to initial concentration of the protein and first order with respect to acid concentration. The results show that glue degradation under normal tankhouse operation should be rapid, with degradation to number-average molar mass (M n ) < 10,000 units occurring in about 40 to 80 minutes depending on the mass transfer rate (or mixing) of the electrolyte solution. Samples of glue from three different sources showed almost no difference in degradation rates. Results calculated from the rate equation for glue degradation have been correlated with cathode polarization data from the literature, and the results suggest that critical glueM n below which the glue loses most of its activity is 3700.
Similar content being viewed by others
References
A.R. Despic and K.I. Popov: inModern Aspects of Electrochemistry, B.E. Conway and J.O'M. Bockris, eds., Butterworth's, London, 1972, no. 7, pp. 199–313.
D.R. Turner and G.R. Johnson:J. Electrochem. Soc., 1962, vol. 109, pp. 798–804.
O. Kardos and D. G. Foulke: inAdvances in Electrochemistry and Electrochemical Engineering, P. Delahay and C. Tobias, eds., Wiley-Interscience, New York, NY, 1962, vol. 2, pp. 145–233.
N.R. Bharucha, Z. Zavorsky, and R.L. Leroy:Metall. Trans. B, 1978, vol. 9B, pp. 509–14.
T.C. Franklin:Surf. Technol., 1987, vol. 30, pp. 415–28.
J. Laezza, R. Box, and J.D. Scott:Int. Symp. on Electrometallurgy in Plant Practice, Montreal, Oct. 21-24, 1990, paper 1.1.
R. Winand, G. Troch, M. Gerges, and P. Harlet: inProcess Metallurgy 3, I.H. Warren, ed., Elsevier Science Publishers B.V., Amsterdam, 1984, pp. 133–45.
T.F. Mitchell:Kirk Othmer Encyclopedia of Chemical Technology, 3rd ed., H.F. Mark, D.F. Othmer, C.G. Overberger, and G.T. Seaborg, eds., John Wiley, New York, NY, 1979, vol. 11, pp. 911–20.
E.H. Chia and Y.T. Su:J. Met., Apr. 1987, pp. 42-45.
J. Kristiansen and A. Hvidt:Acta Chem. Scand., 1990, vol. 44, pp. 144–46.
T.N. Andersen, R.D. Budd, and R.W. Strachan:Metall. Trans. B, 1976, vol. 7B, pp. 333–38.
S. Krzewska, L. Pajdowski, H. Podsaidly, and J. Podsaidly:Metall. Trans. B, 1984, vol. 15B, pp. 451–59.
S. Hjerten:Electrophoresis, 1990, vol. II, pp. 665–90.
H. Shirahama and T. Suzawa:J. Colloid Interface Sci., 1988, vol. 126, pp. 269–77.
M. Degrez, J.-L. Delplancke, and R. Winand:J. Appl. Electrochem., 1990, vol. 20, pp. 110–15.
T. Hashimoto, H. Sasaki, M. Aiura, and Y. Kato:J. Chromat., 1978, vol. 160, pp. 301–05.
Y. Kato, K. Komiya, H. Sasaki, and T. Hashimoto:J. Chromat., 1980, vol. 193, pp. 311–15.
W.W. Yau, J.J. Kirkland, and D.D. Bly:Modern Size-Exclusion Liquid Chromatography, John Wiley, New York, NY, 1979, pp. 315–43.
T.D. Schlabach:Anal. Biochem., 1984, vol. 139, pp. 309–15.
H.-G. Elias:Macromolecules 2, Plenum Press, New York, NY, 1977, p. 1059.
W.W. Yau and D.D. Bly:Size Exclusion Chromatography (GPC), T. Provder, ed., American Chemical Society, Washington, DC, 1979, vol. 138, pp. 197–206.
Encyclopedia of Industrial Chemical Analysis, Interscience Publishers, New York, NY, 1966, vol. 13, pp. 487-94.
J.E. Eastoe:Biochem. J., 1955, vol. 61, pp. 589–600.
N. Ibl:Electrochim. Acta, 1977, vol. 22, pp. 456–77.
S. Yakamoto, K. Nakanishi, and R. Matsuno:Ion Exchange Chromatography of Proteins, Marcel Dekker, New York, NY, 1988, p. 359.
O. Kardos and D.G. Foulke:Advances in Electrochemistry and Electrochemical Engineering, P. Delahay and C. Tobias, eds., Wiley-Interscience, New York, NY, 1962, vol. 2, p. 145.
H.M. Wang, S.F. Chen, T.J. O'Keefe, M. Degrez, and R. Winand:J. Appl. Electrochem., 1989, vol. 19, pp. 174–82.
B.E. Langer and P. Stantke:Proc. Int. Symp. on Productivity and Technology in the Metallurgical Industries, Cologne, Sept. 17-22, 1989, TMS, Warrendale, PA, p. 717.
Author information
Authors and Affiliations
Additional information
Formerly Postdoctoral Fellow with the Department of Chemistry, University of Saskatchewan
Rights and permissions
About this article
Cite this article
Saban, M.D., Scott, J.D. & Cassidy, R.M. Collagen proteins in electrorefining: Rate constants for glue hydrolysis and effects of molar mass on glue activity. Metall Trans B 23, 125–133 (1992). https://doi.org/10.1007/BF02651848
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02651848