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Composition and structure of a galactomannan macromolecule from seeds ofAstragalus lehmannianus bunge

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Abstract

The composition and structure of a galactomannan from seeds ofAstragalus lehmannianus, an endemic legume species, is reported for the first time. The purified galactomannan (yield, 4.8%) contained 55% D-mannose and 45%D-galactose and had a molecular weight of 997.03 kDa. Its aqueous solutions were optically active and highly viscous (the specific rotation [α]D equaled +81.3°; the characteristic viscosity [η], 868.4 ml/g). Chemical, Chromatographic, and spectral (IR and13C-NMR spectroscopy) methods were used to demonstrate that the main chain of the molecule is formed by residues of 1,4-β-D-mannopyranose, 78% of which are substituted at position 6 with single α-D-galactopyranose. The distribution of galactose along the chain was calculated from NMR spectra: frequencies of occurrence per pair of neighboring mannose units of ( 1 ) two substituents, (2) one substituent, and (3) no substituents were 65.3%, 31.5, and 3.2, respectively. The specific rotation of the galactomannans was shown to correlate with their content of galactose.

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  1. N. M. Mestechkina, O. V. Anulov, N. I. Smirnova & V. D. Shcherbukhin

    Present address: Russian Academy of Sciences, Bach Institute of Biochemistry, Leninskiipr. 33, 117071, Moscow, Russia

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    1. N. M. Mestechkina
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    2. O. V. Anulov
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    3. N. I. Smirnova
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    4. V. D. Shcherbukhin
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    Mestechkina, N.M., Anulov, O.V., Smirnova, N.I. et al. Composition and structure of a galactomannan macromolecule from seeds ofAstragalus lehmannianus bunge. Appl Biochem Microbiol 36, 502–506 (2000). https://doi.org/10.1007/BF02731898

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