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Chromate metabolism in liver microsomes

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Abstract

The carcinogenicity and mutagenicity of various chromium compounds have been found to be markedly dependent on the oxidation state of the metal. The carcinogen chromate was reduced to chromium(III) by rat liver microsomes in vitro. Metabolism of chromate by microsomal enzymes occurred only in the presence of either NADPH or NADH as cofactor. The chromium(III) generated upon metabolism formed a complex with the NADP+ cofactor. Significant binding of chromium to DNA occurred only when chromate was incubated in the presence of microsomes and NADPH. Specific inhibitors of the mixed function oxidase enzymes, 2′-AMP, metyrapone, and carbon monoxide, inhibited the rate of reduction of chromate by microsomes and NADPH. The possible relationship of metabolism of chromate and its interaction with nucleic acids to its carcinogenicity and mutagenicity is discussed.

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References

  1. NIOSH,Criteria for a Recommended Standard: Occupational Exposure to Chromium(VI), USDHEW, Public Health Service Center for Disease Control, 1975.

  2. National Research Council, Committee on Biologic Effects of Atmospheric Pollutants,Chromium, Washington, D.C., National Academy of Sciences, 1974.

    Google Scholar 

  3. P. E. Enterline,J. Occup. Med. 16, 523 (1974).

    PubMed  CAS  Google Scholar 

  4. A. Hedenstedt, D. Jenssen, B.-M. Lidesten, C. Ramel, U. Rannug, and R. M. Stern,Scand. J. Work Environ. Health 3, 203 (1977).

    PubMed  CAS  Google Scholar 

  5. A. M. Baetjer,Arch. Ind. Hyg. Occup. Med. 2, 487 (1950).

    CAS  Google Scholar 

  6. S. Langard and T. Norseth,Br. J. Ind. Med. 32, 62 (1975).

    PubMed  CAS  Google Scholar 

  7. F. W. Sunderman, Jr.,Food Cosmet. Toxicol. 9, 105 (1971).

    PubMed  CAS  Google Scholar 

  8. A. Furst, M. Schlauder, and D. P. Sasmore,Cancer Res. 36, 1779 (1976).

    PubMed  CAS  Google Scholar 

  9. M. Kuschner and S. Laskin, inMorphology of Experimental Respiratory Carcinogenesis, P. Nettsheim, M. G. Hanna, and J. W. Deatherage, eds., USAEC, Office of Information Services, Washington, D.C., pp. 203–225 (1970).

    Google Scholar 

  10. B. P. Lane and M. J. Mass,Cancer Res. 37, 1476 (1977).

    PubMed  CAS  Google Scholar 

  11. G. D. Stoner, M. B. Shimkin, M. C. Troxell, T. L. Thompson, and L. S. Terry,Cancer Res. 36, 1744 (1976).

    PubMed  CAS  Google Scholar 

  12. P. Nettesheim, M. G. Hanna, D. G. Doherty, R. F. Newell, and A. Hellman, inMorphology of Experimental Respiratory Carcinogenesis, P. Nettsheim, M. G. Hanna, and J. W. Deatherage, eds., USAEC, Office of Information Services, Washington, D.C., pp. 437–448 (1970).

    Google Scholar 

  13. S. Laskin, M. Kuschner, and R. T. Drew, inInhalation Carcinogenesis, M. G. Hanna, P. Nettsheim, and J. R. Gilbert, eds., USAEC, Office of Information Services, Washington, D.C., pp. 321–350 (1970).

    Google Scholar 

  14. S. Venitt and L. S. Levy,Nature 250, 493 (1974).

    Article  PubMed  CAS  Google Scholar 

  15. H. Nishioka,Mut. Res. 31, 185 (1975).

    CAS  Google Scholar 

  16. F. L. Petrilli and S. De Flora,Appl. Environ. Microbiol. 33, 805 (1977).

    PubMed  CAS  Google Scholar 

  17. G. Löfroth and B. N. Ames,Mut. Res. 53, 65 (1978).

    Google Scholar 

  18. G. Löfroth,Naturwiss. 65, S207 (1978).

    Article  PubMed  Google Scholar 

  19. S. Bonatti, M. Meini, and A. Abbondandolo,Mut. Res. 38, 147 (1976).

    CAS  Google Scholar 

  20. A. Theodotou, R. J. Stretton, A. H. Norbury, and A. G. Massey,Bioinorg. Chem. 5, 235 (1976).

    Article  PubMed  CAS  Google Scholar 

  21. A. Fradkin, A. Janoff, B. P. Lane, and M. Kuschner,Cancer Res. 35, 1058 (1975).

    PubMed  CAS  Google Scholar 

  22. G. Raffetto, S. Parodi, C. Parodi, M. De Ferrari, R. Troiano, and G. Brambilla,Tumori 63, 503 (1977).

    PubMed  CAS  Google Scholar 

  23. A. G. Levis, M. Buttignol, V. Bianchi, and G. Sponza,Cancer Res. 38, 110 (1978).

    PubMed  CAS  Google Scholar 

  24. M. Miyaki, I. Murata, M. Osabe, and T. Ono,Biochem. Biophys. Res. Commun. 77, 854 (1977).

    Article  PubMed  CAS  Google Scholar 

  25. M. A. Sirover and L. A. Loeb,Science 194, 1434 (1976).

    Article  PubMed  CAS  Google Scholar 

  26. S. J. Gray and K. Sterling,J. Clin. Invest. 29, 1604 (1950).

    Article  PubMed  CAS  Google Scholar 

  27. P. C. Rajam, and A.-L. Jackson,Proc. Soc. Exp. Biol. Med. 99, 210 (1958).

    PubMed  CAS  Google Scholar 

  28. C. J. Sanderson,Transplant. 21, 526 (1976).

    CAS  Google Scholar 

  29. P. K. Johnson and M. R. Mardiney,Transplant. 14, 253 (1972).

    Article  CAS  Google Scholar 

  30. P. L. Gutierrez, T. Sarna, and H. M. Swartz,Phys. Med. Biol 21, 949 (1976).

    Article  PubMed  CAS  Google Scholar 

  31. H. Herrmann, and L. B. Speck,Science 119, 221 (1954).

    Article  PubMed  CAS  Google Scholar 

  32. S. Eisinger, R. G. Shulman, and B. M. Szymanski,J. Chem. Phys. 36, 1721 (1962).

    Article  CAS  Google Scholar 

  33. W. M. Walsh, L. W. Rupp, and B. J. Wyluda,Proc. Intern. Conf. Paramagnetic Resonance 2, 836 (1963).

    CAS  Google Scholar 

  34. J. W. Huff, K. S. Sastry, M. P. Gordon, and W. E. C. Wacker,Biochemistry 3, 501 (1964).

    Article  PubMed  CAS  Google Scholar 

  35. A. Danchin,Biochimie 57, 875 (1975).

    Article  PubMed  CAS  Google Scholar 

  36. A. Kowalsky,J. Biol. Chem 244, 6619 (1969).

    PubMed  CAS  Google Scholar 

  37. H. A. Pearson,Blood 22, 218 (1963).

    PubMed  CAS  Google Scholar 

  38. A. M. Baetjer, C. M. Damron, J. H. Clark, and V. Budacz,Arch. Ind. Health 12, 258 (1955).

    CAS  Google Scholar 

  39. M. L. De Phamphilis, and W. W. Cleland,Biochemistry 12, 3714 (1973).

    Article  Google Scholar 

  40. J. E. Gruber, and K. W. Jennette,Biochem. Biophys. Res. Commun. 82, 700 (1978).

    Article  PubMed  CAS  Google Scholar 

  41. E. F. Neufield, N. O. Kaplan, and S. P. Colowick,Biochem. Biophys. Acta 17, 525 (1955).

    Article  Google Scholar 

  42. J. Garcia and K. W. Jennette, unpublished results.

  43. A. G. Hildebrandt,Biochem. Soc. Symp. 34, 79 (1972).

    PubMed  CAS  Google Scholar 

  44. Y. Imai and R. Sato,Biochem. Biophys. Res. Commun 22, 620 (1966).

    Article  PubMed  CAS  Google Scholar 

  45. D. A. Haugen, and M. J. Coon,J. Biol. Chem. 251, 7929 (1976).

    PubMed  CAS  Google Scholar 

  46. K. W. Jennette, unpublished results.

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Jennette, K.W. Chromate metabolism in liver microsomes. Biol Trace Elem Res 1, 55–62 (1979). https://doi.org/10.1007/BF02783843

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