Abstract
Bienzymatic sensors for the determination of esters of choline were prepared by covalent co-immobilization of cholinesterases and choline oxidase on polymer membranes, obtained by radiation-induced copolymerization of 2-hydroxyethyl methacrylate and glycidyl methacrylate at low temperature. Optimization of the covalent attachment of choline oxidase and acetyl-or butyrylcholinesterase to copolymer was explored. The enzyme-modified polymers were applied on platinum electrodes to form amperometric sensors, based on the electrochemical detection of enzymatically developed hydrogen peroxide. Acetyl-, acetylthiobutyryl-, and butyrylthiocholine contents in standard solutions were measured, and linear calibration curves were determined. Temperature and pH effects on the electrochemical response are described.
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References
Yao, T. (1983),Anal. Chim. Acta 153, 169–174.
Mascini, M. and Moscone, D. (1986),Anal. Chim. Acta 179, 439–444.
Tamiya, E., Sugiura, Y., Navera, E. N., Miroshita, S., Nakajima, K., Akiyama, A., and Karube, I. (1991),Anal. Chim. Acta 251, 129–134.
Rouillon, R., Mionetto, N., and Marty, J. L. (1992),Anal. Chim. Acta 268, 347–350.
Marty, J. L., Sode, K., and Karube, I. (1989),Anal. Chim. Acta 228, 49–53.
Lopez, Ruiz, B., Dempsey, E., Hua, C., Smyth, M. R., and Wang, J. (1993),Anal. Chim. Acta 273, 425–430.
Huang, Z., Villarta-Snow, R., Lubrano, G. J., and Guibault, G. G. (1993),Anal. Biochem. 215, 31–37.
Navera, E. N., Suzuki, M., Tamiya, E., Takeuchi, T., and Karube, I. (1993),Electroanalysis 5, 17–22.
Kano, K., Morikage, G., Uno, B., Esaka, Y., and Goto, M. (1994),Anal. Chim. Acta 299, 69–76.
Morelis, R. M. and Coulet, P. R. (1990),Anal. Chim. Acta 231, 27–32.
Skladal, P. and Mascini, M. (1992),Biosensors Bioelectron. 7, 335–343.
Bernabei, M., Chiavarini, S., Cremisini, C., and Palleschi, G. (1993),Biosensors Bioeledron. 8, 265–271.
Imato, T. and Ishibashi, N. (1995),Biosensors Bioelectron. 10, 435–441.
Kumaran, S. and Morita, M. (1995),Talanta 42, 649–655.
Cremisini, C., Di Sario, S., Mela, J., Pilloton, R., Palleschi, G. (1995),Anal. Chim. Acta 311, 273–280.
Trojanowicz, M. and Hitchman, M. L. (1996),Trends Anal. Chem. 15, 38–45.
Palchetti, I., Cagnini, A., Del Carlo, M., Coppi, C., Mascini, M., and Turner A. P. F. (1997),Anal. Chim. Acta 337, 315–321.
Doretti, L., Ferrara, D., Barison, G., and Lora, S. (1994),Appl. Biochem. Biotech. 49, 191–202.
Doretti, L., Gattolin, P., and Lora, S. (1994),Anal. Lett. 27, 2455–2470.
Sundberg, L. and Porath, J. (1974),J. Chromat. 90, 87–98.
Kalal, J. (1978),J. Polym. Sci. Polym. Symp. 62, 251–255.
Hannibal-Friedrich, O., Chun, H., and Sernetz, M. (1980),Biotechnol. Bioeng. 22, 157–175.
Svec, F., Kalal, J., Menyailova, I.I., and Nakhapetyan, L. A. (1978),Biotechnol. Bioeng. 20, 1319–1328.
Hall, C. E. and Hall, E. A. H. (1993),Anal. Chim. Acta 281, 645–653.
Hall, C. E. and Hall, E. A. H. (1995),Anal. Chim. Acta 310, 199–210.
Hall, C. E., Datta D., and Hall, E. A. H. (1996),Anal. Chim. Acta 323, 87–96.
Doretti, L., Ferrara, D., Gattolin, P., and Lora, S. (1996),Biosensors Bioelectron. 11, 365–373.
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Doretti, L., Gattolin, P., Burla, A. et al. Covalently immobilized choline oxidase and cholinesterases on a methacrylate copolymer for disposable membrane biosensors. Appl Biochem Biotechnol 74, 1–12 (1998). https://doi.org/10.1007/BF02786882
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DOI: https://doi.org/10.1007/BF02786882