Abstract
Production of Β-glucosidase inAspergillus niger Bl is subjected to catabolic repression by glucose.Aspergillus niger Bl grown on bran as a carbon source secreted Β-glucosidase. The maximum level of the enzyme was reached after 7 d of fermentation. Addition of 1% glucose to the medium suppressed Β-glucosidase production to undetectable levels. In this study, the organic synthesis of a potential inducer of Β-glucosidase production by A.niger Bl’s reported. Isopropyl-1-thio-Β-D-glucopyranoside (IPTGlc) was synthesized using a two-step organic synthesis protocol. The H-NMR data agreed with those reported previously for the galactoside analog. When IPTGlc was added 24 h after inoculation at a final concentration of 0.4 mM, similar levels of Β-glucosidase were reached 3 to 4 d earlier as compared to fermentation without IPTGlc induction. In practice, this may translate to a more efficient method of producing Β-glucosidase from this fungus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dekker, R. F. H. (1986),Biotechnol. Bioengin. 26, 1438–1442.
Kitpreechavanich, V. M., Hayashi, M., and Nagai, S. (1986),Agric. Biol. Chem. 50, 1703–1711.
Yeoh, H. H., Tan, T. K., and Koh, S. K. (1986),Appl. Microbiol. Biotechnol. 25, 25–28.
Sternberg, D., Vijayakumar, P., and Reese, E. T. (1977),Can. J. Microbiol. 23, 139–147.
Woodward, J. and Wiseman, A. (1982),Enz. Microbiol. Technol. 4, 73–79.
Kerns, G., Dalchow, E., Klappach, G., and Meyer, D. (1986),Acta. Biotechnol. 6, 355–359.
Rombouts, F. M. and Pilnik, W. (1978),Proc. Biochem. 13, 9–13.
Shoseyov, O., Bravdo, B., Ikan, R. and Chet, I. (1988),Phytochem. 27, 1973–1976.
Shoseyov, O., Bravdo, B., Siegel, D., Goldman, A., Cohen, S., and Shoseyov, L. (1990),J. Agric. Food. Chem. 39, 1387–1390.
Kastel′yanos, P. S. and Kuhev, O. N. (1988),App. Biochem. Microbiol. 23, 484–487.
Shay, N.(1991), MSc thesis, Hebrew University, Rehovot, Israel.
Allen, P. K. and Sternberg, D. (1980),Biotechnol. Bioengin. Symp. 10, 189–197.
Kerns, G., Okunev, O. N., Ananin, V. M., and Golovlev, E. L. (1987),Acta. Biotechnol. 7, 535–545.
Nisizawa, T., Suzuki, H., and Nisizawa, K. (1972),J. Biochem. 71, 999–1007.
Roy, S. K., Raha, S. K., Dey, S. K., and Chakrabarty, S. L. (1988),Appl. Environ. Microbiol. 22, 2152–2153.
Forsman, C, Behravan, G., Osterman, A., and Jonsson, B. H. (1988),Acta. Chem. Scand. Ser. B. 42, 314–318.
Carlsson, U., Ferskgard, P. O., and Sevensson, S. C. T (1991),Protein Eng. 1, 1019–1020.
Tilbeurgh, H., Loontiens, F. G., De Bruyne, C. K., and Claeyssens, M. (1988), inMethods in Enzymology, vol. 160, Wood, W. A. and Kellogg, S. T. eds., Academic Press, Inc., pp.45–49.
Sidhu, M. S. and Sandhu, D. K. (1984),Can. J. Microbiol. 30, 1377–1382.
Nisizawa, T., Suzuki, H., and Nisizawa, K. (1971),J. Biochem. 70, 387–393.
Nisizawa, T., Suzuki, H., Nakayama, M., and Nisizawa, K. (1971),J. Biochem. 70, 375–385.
Gooden, B., Legon, T., Helvenstein, P., and Penninckx, M. (1989),J. Gen. Microbiol. 135, 285–292.
Yamane, K, Suzuki, H., Hirotani, M., Ozawa, H., and Nisizawa, K. (1970),J. Biochem. 67, 9–18.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Birk, R., Ikan, A., Bravdo, B. et al. Synthesis of lsopropyl-1-thio-Β-D-glucopyranoside (IPTGlc), an inducer ofaspergillus niger b1 Β-glucosidase production. Appl Biochem Biotechnol 66, 25–30 (1997). https://doi.org/10.1007/BF02788804
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788804