Abstract
Saccharomyces diastaticus cells were immobilized onto beech wood chips of different particle size and three pH values. pH values in the range 5.0–6.0, and 1.84–1.92 mm particle size had a positive effect on the immobilization process. The chosen carrier—1.84 mm-sized wood chips adsorbed 150 mg dry cell mass per g dry carrier mass. The Gibbs free energy and the activation energy for the first (monolayer) and second (multilayer) immobilization stages was 4581, 19090 and 8590 J g mol−1, respectively. The kinetics of immobilized cell systems in ethanol production have been studied in a packed bed-reactor. Ethanol production and the respiration quotient (RQ) were at a maximum at a dilution rate of 0.16/h. The reactor was operated under steady-state conditions for 30 d at the dilution rate 0.16/h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amin G., De Mot R., Van Duck K., Verachtert H.: Direct alcoholic fermentation of starchy biomass using amylolytic yeast strains in batch and immobilized cell systems.Appl. Microbiol. Biotechnol. 22, 237–245 (1985).
Banerjee M., Debnath S., Majumdar S.K.: Production of alcohol from starch by direct fermentation.Biotechnol. Bioeng. 32, 831–834 (1988).
Bärbel H.R., Rehm H.L.: Comparison of fermentation properties and specific enzyme activities of free and calcium-alginate-entrappedSaccharomyces cerevisiae.Appl. Microbiol. Biotechnol. 33, 54–58 (1990).
Calleja G.B., Levy-Rick S., Lusena C.V., Nasim A., Moranell F.: Direct and quantitative conversion of starch to ethanol bySchwanniomyces alluvius.Biotechnol. Lett. 4, 534–546 (1982).
Chen C., Dale M.C., Okos M.R.: The long-term effects of ethanol on immobilized cell reactor performance usingKluyveromyces fragilis.Biotechnol. Bioeng. 36, 975–982 (1990a).
Chen C., Dale M.C., Okos M.R.: Minimal nutritional requirements for immobilized yeast.Biotechnol. Bioeng. 36, 993–1001 (1990b).
Dale C., Chen C., Okos M.R.: Cell growth and death rates as factors in the long-term performance, modeling, and design of immobilized cell reactors.Biotechnol. Bioeng. 36, 983–992 (1990).
Debnath S., Bannerjee M., Majumdar S.K.: Production of alcohol from starch by immobilized cells ofSaccharomyces diastaticus in batch and continuous process.Process Biochem. 25, 43–46 (1990).
Dekkers K., De Kok H., Roels I.: Energetics ofSaccharomyces cerevisiae CBS 426: Comparison of an anaerobic and aerobic glucose limitation.Biotechnol. Bioeng. 23, 1023–1035 (1981).
Divies C., Cachon R., Cavin J.F., Prevost H.: Immobilized cell technology in wine production.CRC Crit. Rev. Biotechnol. 14, 135–153 (1994).
Duvnjak Z., Kosaric N.: Ethanol production bySaccharomyces diastaticus, pp. 175–180 inProc. 6th Internat. Fermentation Symposium (M. Moo-Young, C.W. Robinson, Eds). Pergamon Press, London-Canada-Toronto 1981.
Duvnjak Z., Kosaric N., Kliza S.: Production of alcohol from Jerusalem artichoke by yeast.Biotechnol. Bioeng. 24, 2297–2302 (1982).
Frelot D., Moulin G., Galzy P.: Strain selection for the purpose of alcohol production from starch substrates.Biotechnol. Lett. 4, 705–708 (1982).
Ghose T.K., Tyagi D.: Rapid ethanol fermentation of cellulose hydrolysate.Biotechnol. Bioeng. 21, 1387–1420 (1979).
Laluce C., Mattoon J.R.: Development of rapidly fermenting strains ofSaccharomyces diastaticus for direct conversion of starch and dextrins to ethanol.Appl. Environ. Microbiol. 48, 17–25 (1984).
Lamptey L., Robinson C.W., Moo-Young M.: Kinetics of fuel-grade ethanol production in an immobilized-yeast packed-bed bioreactor, pp. 630–633 inProc. 2nd World Congr. Chemical Engineering, Montreal (Canada) 1981.
Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M.: Ethanol production from glucose and xylose by separated and coculture processes using high cell-density systems.Process Biochem. 28, 519–525 (1993a).
Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M.: Effect of culture conditions on the co-fermentation of a glucose and xylose mixture to ethanol by a mutant ofSaccharomyces diastaticus associated withPichia stipitis.Appl. Microbiol. Biotechnol. 39, 760–763 (1993b).
Larsson P.O., Mosbach K.: Alcohol production by magnetic immobilized yeast.Biotechnol. Lett. 1, 501–506 (1979).
Lodder J.: The Yeast, a Taxonomic Study, 2nd ed., pp. 619–621. North-Holland Publ. Co., Amsterdam 1970.
Masschelein C.A., Ryder D.S., Simon J.P.: Immobilized cell technology in beer production.CRC Crit. Rev. Biotechnol. 14, 155–177 (1994).
Miller G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugars.Anal. Chem. 31, 426–428 (1959).
Moo-Young M., Lamptey J., Robinson C.W.: Immobilization of yeast cells on various supports for ethanol production.Biotechnol. Lett. 2, 541–548 (1980).
Pejin D., Razmovski R.: Continuous cultivation of the yeastSaccharomyces cerevisiae at different dilution rates and glucose concentrations in nutrient media.Folia Microbiol. 38, 141–146 (1993).
Satterfield C.N.:Mass Transfer in Heterogeneous Catalysis, pp. 63–80. MIT Press, Massachusetts 1970.
Smith M.J., Van-Ness C.H.:Introduction to Chemical Engineering Thermodynamics, p. 291 McGraw-Hill Book Co., New York 1975.
Tyagi R.D., Gupta S.K., Chand S.: Process engineering studies on continuous ethanol production by immobilizedS. cerevisiae.Process Biochem. 27, 23–32 (1992).
Umbreit W.W., Burris R.H., Stauffer J.R.:Manometric and Biochemical Techniques, pp. 86–145. Burgess Publ. Co., Minnesota 1972.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Razmovski, R., Pejin, D. Immobilization ofSaccharomyces diastaticus on wood chips for ethanol production. Folia Microbiol 41, 201–207 (1996). https://doi.org/10.1007/BF02814700
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02814700