Abstract
Water-soluble acidic polysaccharides—deesterified pectins and carboxy-derivatives of starch—precipitated with calcium ions were tested as precursors of spherical calcium gels. Pectates prepared from apple or citrus pectin, similarly to alginates, are compounds forming spherical calcium gels stable in aqueous medium which have a relatively highly reproducible mass, particle size, water content, shape, mechanical strength and shearing. Both the liquid-solid partition of low- and high-molar-mass solutes and its kinetics proved to be reasonable features. Distribution of pore size in the above materials was estimated. Detailed pictures of surface and of the interior of calcium beads in the scanning electron microscope are presented. The possible use of calcium beads as enzyme carriers, as affinity matrixes and entrapment materials for diffusion chromatography, solids separations and bioindication of a specific water pollution was evaluated. Calcium alginate beads were always used as reference material.
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References
Anonymous:Pharmacia Biotechnology Products, Catalogue 86, p. 138 (1986); cf.Sigma Catalogue 1987, p. 491;Fluka Katalog 1984/85, p. 353.
Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem.72, 248–254 (1976).
Casson D., Emery A.N.: On the elimination of artefactual effects in assessing the structure of calcium alginate cell immobilization gels.Enzyme Microb. Technol.9, 102–106 (1987).
Cheetham P.S.J., Blunt K.W., Bucke C.: Physical studies on cell immobilization using calcium alginate gels.Biotechnol. Bioeng.21, 2155–2168 (1979).
Clark A.H., Ross-Murphy S.D.: Structural and mechanical properties of biopolymer gels.Adv. Polym. Sci.83, 57–192 (1987).
Gemeiner P., Mislovičová D., Šoltés L., Tichá M., Barthová J.: Inhibition of rabbit muscle lactate dehydrogenase by Cibacron Blue 3G-A bound to water-soluble α-glucans.Biológia (Bratislava)39, 763–768 (1984).
Gemeiner P., Mislovičová D., Zemek J., Kuniak L'.: Anthraquinone-triazine derivatives of polysaccharides. Relation between structure and affinity to lactate dehydrogenase.Collect. Czech. Chem. Commun.46, 419–427 (1981).
Kierstan M.: The use of calcium alginate gels for “solids separation” and “diffusional chromatography” of biological materials.Biotechnol. Bioeng.23, 707–716 (1981).
Kierstan M., Darcy G., Reilly J.: Studies on the characteristics of alginate gels in relation to their use in separation and immobilization applications.Biotechnol. Bioeng.24, 1507–1517 (1982).
Kohn R.: Ion binding on polyuronates-alginate and pectin.Pure Appl. Chem.42, 371–379 (1975).
Kohn R., Tibenský V.: Determination of carboxyl groups of pectin by precipitation of copper pectates and pectinates. (In Slovak)Chem. Zvesti19, 98–106 (1965).
Kohn R., Tihlárik K.: Binding of calcium and lead ions to carboxystarch prepared by action of nitrogen dioxide on native starch and its 2,3-dialdehyde derivatives.Collect. Czech. Chem. Commun.51, 1340–1351 (1986).
Mattiasson B.: Immobilization methods, pp. 3–25 inImmobilized Cells and Organels, Vol. I (B. Mattiasson, Ed.). CRC Press, Boca Raton 1983.
Mislovičová D., Gemeiner P., Breier A.: Study of porous cellulose beads as an affinity adsorbentvia quantitative measurements of interactions of lactate dehydrogenase with immobilized anthraquinone dyes.Enzyme Microb. Technol.10, 568–573 (1988).
Mislovičová D., Gemeiner P., Šoltés L.: Inhibition of lactate dehydrogenase ex rabbit muscle by Cibacron Blue 3G-A bound to water-soluble hydroxyethylcellulose.Collect. Czech. Chem. Commun.50, 1335–1340 (1985).
Morris E.R., Powell D.A., Gidley M.J., Rees D.A.: Conformations and interactions of pectins. I. Polymorphism between gel and solid states of calcium polygalacturonate.J. Mol. Biol.155, 507 (1982).
Nilsson I., Ohlsson S.: Columnar denitrification of water by immobilizedPseudomonas denitrificans cells.Eur. J. Appl. Microbiol.14, 84–90 (1982).
Owens H.S., Lotzkar H., Schultz T.H., Maclay W.D.: Shape and size of pectinic acid molecules deduced from viscometric measurements.J. Amer Chem. Soc.68, 1628–1632 (1946).
Rees D.A.: Polysaccharide gels. A molecular view.Chem. Ind. 630–636 (1972).
Řeháček Z., Krumphanzl V.: New trends in microbial technology.Folia Microbiol.32, 65–81 (1987).
Somerville H., Mason J.R., Ruffel R.N.: Benzene degradation by bacterial cells immobilized in polyacrylamide gel.Eur. J. Appl. Microbiol.4, 75–85 (1977).
Tanaka H., Matsumura M., Veliky I.A.: Diffusion characteristics of substrates in Ca-alginate gel beads.Biotechnol. Bioeng.26, 53–58 (1984).
Tibenský V., Kohn R.: The method for preparation of sodium or potassium pectate.Czechoslov. Pat. 137 935 (1970).
Tibenský V., Rosík J., Zitko V.: Determination of the degree of esterification of pectin.Nahrung7, 321–325 (1963).
Tomašovičová D., Tóth D., Gemeiner P.: Metabolism of bacterial cells bound to solid carriers. (In Slovak),Biológia (Bratislava)42, 231–237 (1987a).
Tomaŝovičová D., Tóth D., Vraždová Ĺ.: Utilization of immobilized microorganisms for indication of water pollution.Enzyme Technologies, Interbiotech '87, Bratislava 1987b. Abstr. Commun., p. 130.
Tóth D., Tomašovičová D., Gemeiner P.: Immobilization of bacterial cells on solid carriers. (In Slova)Biológia (Bratislava)41, 1097–1104 (1986).
Vojtíšek V., Jirků V.: Immobilized cells.Folia Microbiol.28, 309–340 (1983).
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Gemeiner, P., Kurillová, L., Malovíková, A. et al. Properties of spherical calcium pectate and alginate gels and their use in diffusion chromatography, solids separations and immobilization of enzymes and cells. Folia Microbiol 34, 214–227 (1989). https://doi.org/10.1007/BF02821295
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DOI: https://doi.org/10.1007/BF02821295