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Hydrolysis of the nitrile group in α-aminophenylacetonitrile by nitrilase; Development of a new biotechnology for stereospecific production ofS-α-phenylglycine

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Abstract

Phenylglycine was obtained as the sole metabolite when α-aminophenylacetonitrile was ted to the culture broth ofAspergillus furmigatus. The isolated phenylglycine showed L-configuration with 80% optical purity. Examination of the hydrolysis, of the substrate to phenylglycine with cell free extracts, and the supernatant fraction and the particulate fraction both of which were obtained after ultracentrifugation of the cell free extract at 100,000g, indicated that the nitrile group hydrolyzing enzymes, nitrilase existed not only in cytoplasm, but in microsome fractions.

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Authors and Affiliations

  1. College of Pharmacy, Seoul National University, Kwanakku, 151, Seoul, Korea

    Soo Young Choi & Yang M. Goo

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  1. Soo Young Choi
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  2. Yang M. Goo
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Choi, S.Y., Goo, Y.M. Hydrolysis of the nitrile group in α-aminophenylacetonitrile by nitrilase; Development of a new biotechnology for stereospecific production ofS-α-phenylglycine. Arch. Pharm. Res. 9, 45–47 (1986). https://doi.org/10.1007/BF02857706

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  • DOI: https://doi.org/10.1007/BF02857706

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