Abstract
Enzymatic hydrolysis of SO2-impregnated, steam-explodedEucalyptus viminalis was carried out at increasing substrate concentrations and enzyme loadings. When low enzyme loadings were used, the peroxide-treated fraction derived from eucalyptus chips (SEE-WIA/ H2O2) was more readily hydrolyzed than the water-insoluble (SEE-WI) and the alkali-insoluble (SEE-WIA) fractions. The various cellulosic fractions derived from steam-explodedE. viminalis exhibited a greater susceptibility to hydrolysis than the equivalent aspen and spruce substrates, particularly at high substrate concentrations (10%, w/v).
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References
Tassinari, T., Macy, C., and Spano, L. (1980),Biotechnol. Bioeng. 22, 1689- 1705.
Saddler, J. N., Brownell, H. H., Clermont, L. P., and Levitin, N. (1982),Biotechnol. Bioeng. 24, 1389–1402.
Mamers, H. and Menz, D. N. J. (1984),Appita 37, 644–649.
Gould, J. M. (1984),Biotechnol. Bioeng. 26, 46–52.
Clark, T. A. and Mackie, K. L. (1987),J. Wood. Chem. Technol. 7, 373–403.
Chum, H. L., Johnson, D. K., Black, S. K., and Overend, R. P. (1990),Appl. Biochem. Biotechnol. 24/25, 1–14.
Tenrud, I. E., Theander, O., Torneport, L., and Vallander, L. (1989),Enzyme Microb. Technol 11, 500–506.
Dekker, R. F. H. and Wallis, A. F. A. (1983),Biotechnol. Bioeng. 25, 3027–3048.
Torget, R. Werdene, P., Himmel, M., and Grohmann, K. (1990),Appl. Biochem. Biotechnol. 24/25, 115–126.
Eklund, R., Galbe, M., and Zacchi, G. (1990),Enzyme Microb. Technol. 12, 225–228.
Dekker, R. F. H., Karageorge, H., and Wallis, A. F. A. (1987),Biocatalysis 1, 45–59.
Martinez, J., Negro, M. J., Saez, F., Manero, J., Saez, R., and Martin, C. (1990),Appl. Biochem. Biotechnol. 24/25, 127–134.
Schwald, W., Breuil, C., Brownell, H. H., Chan, M., and Saddler, J. N. (1989),Appl. Biochem. Biotechnol. 20/21, 29–44.
Ramos, L. P., Breuil, C., and Saddler, J. N. (1990),Holzforschung (in press.)
Schwald, W., Smaridge, T., Chan, M., Breuil, C., and Saddler, J. N. (1989),Enzyme Systems for Lignocellulose Degradation, Coughlan, M. P., ed., Elsevier, New York, pp. 231–242.
Brownell, H. H., Schwald, W., Smaridge, T., and Saddler, J. N. (1988),Proceedings of the Workshop on the Bioconversion of Lignocellulosics, Ottawa, Ont., Forintek Canada Co., p. A-1.
Saddler, J. N. (1986),Microbiol. Sci. 3, 84–87.
Ooshima, H., Burns, D. S., and Converse, A. O. (1990),Biotechnol. Bioeng. 36, 446–452.
Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985),J. Wood. Chem. Technol. 5, 405–425.
Schwald, W., Chan, M., Breuil, C., and Saddler, J. N. (1988),Appl. Microbiol. Biotechnol. 28, 398–403.
Breuil, C., Chan, M., and Saddler, J. N. (1990),Appl. Microbiol. Biotechnol. 34, 31–35.
Chan, M., Breuil, C., Schwald, W., and Saddler, J. N. (1989),Appl. Microbiol. Biotechnol. 31, 413–418.
Ghose, T. K. (1987),Pure Appl. Chem. 59, 257–268.
Yamashiki, T., Matsui, T., Saitoh, M., Matsuda, Y., Okajima, K., and Kamide K. (1990),British Polym. J. 22, 201–212.
Mushua, Y. and Goring, D. I. A. (1975),Wood Sci. Technol. 9, 45–58.
Barbour, R. J. and Leney, L. (1981),Proceedings of the Waterlogged Wood Working Group Conference, Grattan, D. W., ed., The International Council of Museums, Ottawa, Ont., pp. 208–225.
Ucar, G. and Fengel, D. (1988),Holzforschung 42, 141–148.
Wong, K. K. Y., Deverell, K. F., Mackie, K. L., Clark, T. A., and Donaldson, L. A. (1988),Biotechnol. Bioeng. 31, 447–456.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02950784.
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Ramos, L.P., Breuil, C. & Saddler, J.N. Comparison of steam pretreatment of eucalyptus, aspen, and spruce wood chips and their enzymatic hydrolysis. Appl Biochem Biotechnol 34, 37–48 (1992). https://doi.org/10.1007/BF02920532
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DOI: https://doi.org/10.1007/BF02920532