Abstract
Glucosyltransferase activities, produced by batch culture ofLeuconostoc mesenteroides NRRL B-1299, were recovered both in the culture supernatant (SGT) and associated with the insoluble part of the culture (IGT). A total glucosyltransferase activity of 3.5 U/mL was measured in batch culture. The enzymes from the supernatant were purified 313 times using aqueous two-phase partition between dextran and PEG phases, yielding a preparation with 18.8 U/mg protein. It was shown that both SGT and IGT preparations catalyze acceptor reactions and transfer the glucose unit from sucrose onto maltose to produce glucooligosaccharides. Some of the glucooligosaccharides synthesized (Ln series) contain α-(l→6) osidic linkages and a maltose residue at the reducing end. They were completely hydrolyzed by glucoamy-lase and dextranase. The other glucooligosaccharides synthesized (Bn series) resisted the action of these enzymes. The tetrasaccharide of this series has been characterized by13C NMR. Its structure was determined as 2–O–α–D–glucosylpanose. The oligosaccharides synthesized by the maltose acceptor reaction with the SGT and IGT preparations only differed in the relative amounts in which they were produced. The difference may arise from diffusional limitations appearing when the insoluble catalyst is used. Under the assay conditions, the glucanase resistant oligosaccharide yield was 35% with both glucosyltrans-ferase preparations.
Similar content being viewed by others
References
Morgan, A. J., Mul, A. J., Beldman, G., and Voragen, A. G. J. (1992),Agr. Food-Ind. Hi-Tech. 11/12, 35.
Monsan, P., Paul, F., Remaud, M., and Lopez, A. (1989),Food Biotech. 3,11.
Hidaka, H., Hirayama, M., Tokunaga, T., and Eida, T. (1990), inNew Developments in Dietary Fiber, Furda, I., and Brine, C. J., eds., Plenum, New York, pp. 105–117.
Sherman-Gold, R. (1991),Genet. Eng. News 2, 1.
Rademacher, T. W. (1992),TIBTEC 10, 227.
Fujii, S. and Komoto, M. (1991),Zuckerind. 116, 197.
Ajisaka, K., Nishida, H., and Fujimoto, H. (1987),Biotech. Lett. 9, 387.
Horikoshi, K., Nakamura, N., Matsuzawa, N., and Yamamoto, M. (1981), inProceedings of the First International Symposium on Cyclodextrins, Szejtli, J., ed., D. Reidel Publishing Co., Dordrecht, Holland, pp. 25–39.
Koepsell, H. J., Tsuchiya, H. M., Hellman, N. N., Kasenko, A., Hoffman, C. A., Sharpe, E. S., and Jackson, R. W. (1952),J. Biol. Chem. 200, 793.
Tsuchiya, H. M., Hellman, N. N., and Koepsell, H. J. (1953),J. Am. Chem. Soc. 75, 757.
Hehre, E. J. (1953),J. Am. Chem. Soc. 75, 4866.
Jeanes, A., Haynes, W. C., Wilham, C. A., Rankin, J. C, Melvin, E. H., Austin, M. J., Cluskey, J. E., Fisher, B. E., Tsuchiya, H. M., and Rist, C. E. (1954),J. Am. Chem. Soc. 76, 5041.
Seymour, F. R., Knapp, R. D., and Bishop, S. H. (1976),Carbohydr. Res. 51, 179.
Larm, O., Lindberg, B., and Svensson, S. (1971),Carbohydr. Res. 20, 39.
Paul, F., Oriol, E., Auriol, D., and Monsan, P. (1986),Carbohydr. Res. 149, 433.
Remaud, M., Paul, F., Monsan, P., Heyraud, A., and Rinaudo, M. (1991),J. Carbohydr. Chem. 10, 861.
Lopez-Munguia, C. A., Pelenc, V., Remaud, M., Paul, F., Monsan, P., Biton, J., Michel, J. M., and Lang, C. (1990), inEnzyme Engineering 10, vol. 613, Laskin, A. L, Mosbach, K., Thomas, D., and Wingard, Jr.L. B., eds.,Ann. NY Acad. Sci, New York, pp. 717–722.
Pelenc, V., Lopez-Munguia, C. A., Remaud, M., Biton, J., Michel, J. M., Paul, F., and Monsan, P. (1991),Sci. Aliments 11, 465.
Remaud, M., Paul, F., Monsan, P., Lopez-Munguia, A., Vignon, M. (1992),J. Carbohydr. Chem. 11, 359.
Paul, F., Lopez-Munguia, A., Remaud, M., Pelenc, V., and Monsan, P. (1992),Eur. Patent 0, 325, 872.
Seymour, F. R., Slodki, M. E., Plattner, R. D., and Jeanes, A. (1977),Carbohydr. Res. 53, 153.
Seymour, F. R., Chen, E. C. M., and Bishop, S. H. (1979),Carbohydr. Res. 68, 113.
Seymour, F. R., Knapp, R. D., Chen, E. C. M., Jeanes, A., and Bishop, S. H. (1979),Carbohydr. Res. 71, 231.
Kobayashi, M., Shishido, K., Kikuchi, T., and Matsuda, K. (1973),Agric. Biol. Chem. 37, 357.
Kobayashi, M., Shishido, K., Kikuchi, T., and Matsuda, K. (1973),Agric. Biol. Chem. 37, 2763.
Kobayashi, M. and Matsuda, K. (1974),Biochem. Biophys. Acta 370, 441.
Kobayashi, M. and Matsuda, K. (1976),J. Biochem. 79, 1301.
Kobayashi, M. and Matsuda, K. (1975),Biochem. Biophys. Acta 397, 69.
Kobayashi, M. and Matsuda, K. (1977),Agr. Biol. Chem. 41, 1931.
Smith, E. E. (1970),FEBS Lett. 12, 33.
Hehre, E. J. and Suzuki, H. (1966),Arch. Biochem. Biophys. 113, 675.
Paul, F., Monsan, P., and Auriol, D. (1984),Eur. Patent 0,125,981.
Sumner, J. B. and Howell, S. F. (1935),J. Biol. Chem. 108, 51.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265.
Bourne, E. J., Hutson, D. H., and Weigel, H. (1963),Biochem. J. 86, 555.
Taylor, C, Cheetham, N. W. H., and Walker, G. J. (1985),Carbohydr. Res. 137, 1.
Pazur, J. H. and Ando, T. (1960),J. Biol. Chem. 235, 297.
Pazur, J. H. and Kleppe, K. (1962),J. Biol. Chem. 237, 1002.
Coxon, B. (1980),Dev. Food Carbohydr. 2, 351.
Heyraud, A., Rinaudo, M., Vignon, M. R., and Vincendon, M. (1979),Biopolymers 18, 167.
Dorman, D. E. and Roberts, J. D. (1971),J. Am. Chem. Soc. 93, 4463.
Seymour, F. R., Knapp, R. D., Bishop, S. H., and Jeanes, A. (1979),Carbohydr. Res. 68, 123.
Seymour, F. R., Knapp, R. D., and Bishop, S. H. (1979),Carbohydr. Res. 72, 229.
Valette, P., Pelenc, V., Djouzy, Z., Andrieux, C, Paul, F., Monsan, P., and Szylit, O. (1993),J. Sci. Food. Agric. 62, 121.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Remaud-Slmeon, M., Lopez-Munguia, A., Pelenc, V. et al. Production and use of glucosyltransferases fromLeuconostoc mesenteroides NRRL B-1299 for the synthesis of oligosaccharides containing α-(1→2) linkages. Appl Biochem Biotechnol 44, 101–117 (1994). https://doi.org/10.1007/BF02921648
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02921648