Abstract
Formic acid pretreatment onPinus radiata D. Don was studied in order to improve the cellulose hydrolysis by commercial cellulase. The formic acid treatment effectively removed the lignin. A low substitution (formylation) and a crystallinity decrease of the cellulose in the pulp were observed. As consequence of these parameter changes, owing to the formic acid pretreatment on sawdust, a higher saccharification value was observed. The degree of saccharification increased when the degree of substitution (measured by titration) decreased and the portion of amorphous cellulose (measured via an X-ray technique) increased.Trichoderma reesei cellulase hydrolyzed the untreated and pretreated Pinus sawdust with formic acid in 25% and 56% of saccharification, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Johansson, A., Aaltonen, O., and Ylinen, P. (1987),Biomass 13, 45–65.
Aziz, S., McDonough, T., Thompson, N., and Doshi, M. R. (1988),TAPPI J. 71, 251–254.
Baeza, J., Freer, J., Pedreros, A., Schmidt, E., Mansilla, H., and Durán, N. (1990),Bol. Soc. Chil. Quitn. 35, 331–337.
Freudenberg, K., Janson, A., Knopf, E. and Haag, A. (1936),Berichte 69B, 1415–1425.
Staudinger, H. and Dreher, E. (1936),Berichte 69B, 1729–1739.
Wright, G. F. and Hibbert, H. (1937),J. Am. Chem. Soc. 59, 125–130.
Bucholtz, M. and Jordan, R. K. (1983),Pulp and Paper 57, 102–104.
Jordan, R. K. (1982),PCT Int. Appl. WO 8201, 902.
Baeza, J., Freer, J., Pedreros, A., and Belmar, H. (1987), Proc. VI National Meeting of Research and Development of Forestry Products, Chile, pp. 163–174.
Sapag-Hagar, J., Dimitroff, N., and Piffaut, O. (1985), Proc. Symposium of Chemistry and National Development Chilean Chem. Soc., July 1985, Concepción, Chile, pp. 132ó136.
Mamers, H. and Menz, D. N. J. (1984),APPITA 37, 644–649.
Clark, T. A. and Mackie, K. L. (1987),J. Wood Chem. Technol. 7, 373–403.
Hohlberg, A. I., Aguilera, J. M., Agosin, E., and San Martin, R. (1989),Biomass 18, 81–93.
Dekker, R. F. H. (1987),Biocatalysis 1, 63–75.
Baeza, J., Freer, J., Palma, G., Pedreros, A., Rodriguez, J., Rojas, N., and Durán, N. (1985), Proc. Symposium of Chemistry and Renewable Resources Utilization, Chilean Chem. Soc., July 1985, Concepcián, Chile, pp. 63–66.
Baeza, J., Freer, J., Pedreros, A., and Durán, N. (1988), Proc. XVIII Latin American Chemical Congress, January 1988, Santiago, Chile, pp. 513, 514.
Durán, N., Ferraz, A., Freer, J., Pedreros, A., and Baeza, J. (1988), FENABIO-88, First National Conference of Biotechnology, April 1988, R. J. Brazil.
Fujimoto, T., Takahashi, S.-I., Tsuji, M., Miyamoto, T., and Inagaki, H. (1986),J. Polym. Sci. Part C, Polym. Lett. 24, 495–501.
Gharpuray, M. M., Lee, Y. H., and Fan, L. T. (1983),Biotechnol. Bioeng. 25, 157–172.
Grethlein, H. E. (1985),Bio/Technology, Feb. pp. 155–160.
Mandels, M., Andreotti, R., and Roche, C. (1976),Biotechnol. Bioeng. Symp. 6, 21–33.
Commission on Biotechnology, IUPAC (1987),Pure & Appl. Chem. 59, 245–256.
Miller, G. L. (1959),Anal. Chem. 31, 426–428.
Updegraff, D. M. (1969),Anal. Biochem. 32, 420–424.
TAPPI Standard T-222 OM-88. Acid-Insoluble lignin in Wood and Pulp 1988.
Simionescu, C. I., Popa, V. I., Rusan, V., and Rusan, M. (1985),Cellulose Chem. Technol. 19, 525–530.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baeza, J., Fernández, A.M., Freer, J. et al. Organosolv-pulping III. Appl Biochem Biotechnol 31, 273–282 (1991). https://doi.org/10.1007/BF02921754
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02921754