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Temperature affects the production, activity and stability of ligninolytic enzymes inPleurotus ostreatus andTrametes versicolor

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Abstract

Enzyme activity was determined in cultures ofPleurotus ostreatus andTrametes versicolor with cellulose as a sole C source and high C/N ratio. The fungi were able to grow and produce laccase and Mn-peroxidase (MnP) at 5–35 °C, the highest production being recorded at 25–30 °C inP. ostreatus and at 35 °C inT. versicolor. Production of both enzymes at 10 °C accounted only for 4–20 % of the maximum value. Temperature optima for enzyme activity were 50 and 55 °C forP. ostreatus andT. versicolor laccases, respectively, and 60 °C for MnP. Temperatures causing 50 % loss of activity after 24 h were 32 and 47 °C for laccases and 36 and 30 °C for MnP fromP. ostreatus andT. versicolor, respectively.

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Abbreviations

ABTS:

2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid

DMAB:

3,3-dimethylaminobenzoic acid

PO:

Pleurotus ostreatus

MBTH:

3-methyl-2-benzothiazolinone hydrazone

MnP:

manganese peroxidase

TV:

Trametes versicolor

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Authors and Affiliations

  1. Laboratory of Biochemistry of Wood-Rotting Fungi, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    J. Šnajdr & P. Baldrian

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  1. J. Šnajdr
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  2. P. Baldrian
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Correspondence to P. Baldrian.

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This study was supported by theGrant Agency of the Academy of Sciences of the Czech Republic (no. B600 200 516), by theMinistry of Education of the Czech Republic (LC06066 and OC155/868) and by the Institutional Research Concept of theInstitute of Microbiology, Academy of Sciences of the Czech Republic (no. AV0Z 5020 0510).

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Šnajdr, J., Baldrian, P. Temperature affects the production, activity and stability of ligninolytic enzymes inPleurotus ostreatus andTrametes versicolor . Folia Microbiol 52, 498–502 (2007). https://doi.org/10.1007/BF02932110

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