Abstract
Effects of culture conditions on the growth of mycelium and the production of intracellular cordycepin ofCordyceps militaris were investigated in this study. The natural medium which is a mixture of 53% brown rice paste, 6% beerwort and 42% soybean meal juice was used for the fermentation in shaking flasks. Fermentation temperature, pH and medium capacity, which had been proved to be significant factors, were optimized by Box-Behnken design. Results showed that dry mycelia weight (DMW) and cordycepin yield (CY) was varied with the transformation of culture conditions. The highest DMW (19.1 g/L) and CY (1.8 mg/g) would be obtained at the condition of fermentation temperature 28 °C, pH 6.2 and medium capacity 57 mL.
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Xie, C., Liu, G., Gu, Z. et al. Effects of culture conditions on mycelium biomass and intracellular cordycepin production ofCordyceps militaris in natural medium. Ann. Microbiol. 59, 293–299 (2009). https://doi.org/10.1007/BF03178331
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DOI: https://doi.org/10.1007/BF03178331