Abstract
Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of ferulic acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of ferulic acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of ferulic acid.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 11:286–290
Borneman WS, Akin DE, VanEseltine WP (1986) Effect of phenolic monomers on ruminal bacteria. Appl Environ Microbiol 52:1331–1339
Borneman WS, Ljungdahl LG, Hartley RD, Akin DE (1991) Isolation and characterisation of p-coumaroyl esterase from the anaerobic fungus Neocallimastix strain MC-2. Appl Environ Microbiol 57:2337–2344
Borneman WS, Ljungdahl LG, Hartley RD, Akin DE (1992) Purification and partial characterisation of two feruloyl esterases from the anaerobic fungus Neocalimastix strain MC-2. Appl Environ Microbiol 58:3762–3766
Castanares A, McCrea SI, Wood TM (1992) Purification and properties of a feruloyl/p-coumaroyl esterase from the fungus Penicillium pinophilum. Enzyme Microb Technol 14:875–884
Colquhoun IJ, Ralet MC, Thibault JF, Faulds CB, Williamson G (1994) Structure identification of feruloylated oligosaccharides from sugar-beet pulp by NMR spectroscopy. Carbohydr Res 263:243–256
Faulds CB, Williamson G (1991) The purification and characterisation of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes. J Gen Microbiol 137:2339–2345.
Faulds CB, Williamson G (1993a) Release of ferulic acid from plant polysaccharides by ferulic acid esterase from Streptomyces olivochromogenes. Carbohydr Polymers 21:153–155
Faulds CB, Williamson G (1993b) Ferulic acid esterase from Aspergillus niger:purification and partial characterisation of two forms from a commercial source of pectinase. Biotechnol Appl Biochem 17:349–359
Faulds CB, Williamson G (1994) Purification and characterisation of a ferulic acid esterase (FAE-III) from Aspergillus niger:specificity for the phenolic moiety and binding to microcrystalline cellulose. Microbiology 140:779–787
Faulds CB, Ralet MC, Williamson G, Hazlewood GP, Gilbert HJ (1995) Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa. Biochim Biophys Acta 1243:265–269
Ferreira LMA, Wood TM, Williamson G, Faulds CB, Hazlewood GP, Gilbert HJ (1993) A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose binding domain. Biochem J 294:349–355
Fry SC (1982) Phenolic components of the primary cell wall. Biochem J 203:493–504
Gibson TS, McCleary BV (1987) A simple procedure for the largescale purification of \-D-xylanase from Trichoderma viride. Carbohydr. Polymers 7:225–240
Huang L, Hseu TH, Wey TT (1991) Purification and characterisation of an xylanase from Trichoderma koningii G-39. Biochem J 278:329–333
Johnson KG, Harrison BA, Schneider H, MacKenzie CR, Fontana JD (1988) Xylan-hydrolysing enzymes from Streptomyces spp. Enzyme Microb Technol 10:403–409
Kato Y, Nevins DJ (1985) Isolation and identification of O-(5-O-feruloyl-L-arabinofuranosyl)-(1,3)-O-\-D-xylopyranosyl-(1,3)-xylopyranose as a component of Zea shoot cells. Carbohydr Res 137:139–150
Kellet LE, Poole DM, Ferreira LMA, Durrant AJ, Hazlewood GP, Gilbert HJ (1990) Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contains identical cellulose-binding domains and are encoded by adjacent genes. Biochem J 272:369–376
Miller GL (1959) Use of dinitrosalicylic acid for determination of reducing groups. Anal Chem 31:426–428
Mueller-Harvey I, Hartley RD, Harris PJ, Curzon EH (1986) Linkage of p-coumaroyl and feruloyl groups to cell-wall polysaccharides of barley straw. Carbohydr Res 148:71–85
Okamura S, Watanabe M (1982) Purification and properties of hydroxycinnamic acid ester hydrolase from Aspergillus japonicus. Agric Biol Chem 46:1839–1848
Poutanen K (1988) Characterisation of xylonolytic enzymes for potential applications. Publication 47, Technical Research Centre of Finland, Espoo.
Puls J, Poutanen K. (1989) Mechanisms of enzymatic hydrolysis of hemicelluloses (xylans) and procedures for determination of the enzyme activities involved. In Coughlan MP (ed) Enzyme systems for lignocellulose degradation. Elsevier, Amsterdam. pp 151–165
Ralet M.C, Thibault JF, Faulds CB, Williamson G (1994a) Isolation and purification of feruloylated oligosaccharides from cell-walls of sugar-beet pulp. Carbohydr Res 263:227–241
Ralet MC, Faulds CB, Williamson G, Thibault JF (1994b) Degradation of feruloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterases from Aspergillus niger. Carbohydr Res 263:257–269
Rombouts FM, Thibault JF (1986) Feruloylated pectic substances from sugar beet pulp. Carbohydr Res 154:177–187
Royer JC, Nakas JP (1991) Purification and characterisation of two xylanases from Trichoderma longibrachiatum. Eur J Biochem 202:521–529
Smith MM, Hartley RD (1983) Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in graminaceous plants. Carbohydr Res 118:65–80
Tenkanen M, Schuseil J, Puls J, Poutanen K (1991) Production, purification and characterisation of an esterase liberating phenolic acids from lignocellulose. J Biotechnol 18:69–84
Ujiie M, Roy C, Yaguchi M (1991) Low-molecular-weight xylanase from Trichoderma viride. Appl Environ Microbiol 57:1860–1862
Viikari L, Kantelinen A, Buchert J, Puls J (1994) Enzymatic accessibility of xylans in lignocellulosic materials. Appl Microbiol Biotechnol 41:124–129
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Faulds, C.B., Williamson, G. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger . Appl Microbiol Biotechnol 43, 1082–1087 (1995). https://doi.org/10.1007/BF00166929
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00166929