Skip to main content
Log in

New isolation of Clostridium aceticum (Wieringa)

  • Laboratory Methods
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

After many attempts to re-isolate Clostridium aceticum (Wieringa) had been unsuccessful, finally a new strain could be isolated that was morphologically and physiologically identical, as could be demonstrated by comparing this strain with the original one, retrieved recently from an old culture collection. Both strains showed the ability to produce cellular materials and acetate from a CO2−H2 gas mixture, as well as from fructose as the substrate. A detailed description of the enrichment and isolation procedures used, is given.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ackman, R. G. 1972. Porous polymer bead packings and formic acid vapor in the GLC of volatile free fatty acids. — J. Chromotogr. Sci. 10: 560–565.

    Google Scholar 

  • Adamse, A. D. 1970. Some characteristics of arthrobacters from a dairy waste activated sludge.—Water. Res. 4: 797–803.

    Google Scholar 

  • Andreesen, J. L., Gottschalk, G. and Schlegel, H. G. 1970. Clostridium formicoaceticum nov.spec. Isolation, description and distinction from C. aceticum and C. thermoaceticum. — Arch. Mikrobiol. 72: 154–174.

    Google Scholar 

  • Balch, W. E. and Wolfe, R. E. 1976. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. — Appl. Environ. Microbiol. 32: 781–791.

    Google Scholar 

  • Balch, W. E., Schoberth, S., Tanner, R. S. and Wolfe, R. S. 1977. Acetobacterium, a new genus of hydrogen-oxidizing, carbon dioxide-reducing, anaerobic bacteria. — Int. J. Syst. Bacteriol. 27: 355–361.

    Google Scholar 

  • Brock, T. D. and O'Dea, K. 1977. Amorphous ferrous sulfide as a reducing agent for culture of anaerobes. — Appl. Environ. Microbiol. 33: 254–256.

    Google Scholar 

  • De Bont, J. A. M., Scholten, A. and Hansen, Th. A. 1980. DNA-DNA hybridization studies of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides and Rhodopseudomonas sulfidophila strains.—In preparation.

  • Hungate, R. E. 1950. The anaerobic mesophilic cellulolytic bacteria. — Bacteriol. Rev. 14: 1–50.

    Google Scholar 

  • Karlsson, J. L., Volcani, B. E. and Barker, H. A. 1948. The nutritional requirements of Clostridium aceticum. — J. Bacteriol. 56: 781–782.

    Google Scholar 

  • Mulder, E. G., Adamse, A. D., Antheunisse, J., Deinema, M. H., Woldendorp, J. W. and Zevenhuizen, L. P. T. M. 1966. The relationship between Brevibacterium linens and bacteria of the genus Arthrobacter. — J. Appl. Bacteriol. 29: 44–71.

    Google Scholar 

  • Ohwaki, K. and Hungate, R. E. 1977. Hydrogen utilization by Clostridia in sewage sludge. — Appl. Environ. Microbiol. 33: 1270–1274.

    Google Scholar 

  • Wieringa, K. T. 1936. Over het verdwijnen van waterstof en koolzuur onder anaërobe voorwaarden. — Antonie van Leeuwenhoek 3: 263–271.

    Google Scholar 

  • Wieringa, K. T. 1939/40. The formation of acetic acid from carbon dioxide and hydrogen by anaerobic spore-forming bacteria. — Antonie van Leeuwenhoek 6: 251–262.

    Google Scholar 

  • Zeikus, J. G. 1977. The biology of methanogenic bacteria. — Bacteriol. Rev. 41: 514–541.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

In memory of Dr Klaas Tammo Wieringa (1891–1980), who has dedicated more than fifty years of his life to many aspects of agricultural microbiology and biotechnology in our laboratory.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adamse, A.D. New isolation of Clostridium aceticum (Wieringa). Antonie van Leeuwenhoek 46, 523–531 (1980). https://doi.org/10.1007/BF00394009

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00394009

Keywords

Navigation