Abstract
After many attempts to re-isolate Clostridium aceticum (Wieringa) had been unsuccessful, finally a new strain could be isolated that was morphologically and physiologically identical, as could be demonstrated by comparing this strain with the original one, retrieved recently from an old culture collection. Both strains showed the ability to produce cellular materials and acetate from a CO2−H2 gas mixture, as well as from fructose as the substrate. A detailed description of the enrichment and isolation procedures used, is given.
Similar content being viewed by others
References
Ackman, R. G. 1972. Porous polymer bead packings and formic acid vapor in the GLC of volatile free fatty acids. — J. Chromotogr. Sci. 10: 560–565.
Adamse, A. D. 1970. Some characteristics of arthrobacters from a dairy waste activated sludge.—Water. Res. 4: 797–803.
Andreesen, J. L., Gottschalk, G. and Schlegel, H. G. 1970. Clostridium formicoaceticum nov.spec. Isolation, description and distinction from C. aceticum and C. thermoaceticum. — Arch. Mikrobiol. 72: 154–174.
Balch, W. E. and Wolfe, R. E. 1976. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. — Appl. Environ. Microbiol. 32: 781–791.
Balch, W. E., Schoberth, S., Tanner, R. S. and Wolfe, R. S. 1977. Acetobacterium, a new genus of hydrogen-oxidizing, carbon dioxide-reducing, anaerobic bacteria. — Int. J. Syst. Bacteriol. 27: 355–361.
Brock, T. D. and O'Dea, K. 1977. Amorphous ferrous sulfide as a reducing agent for culture of anaerobes. — Appl. Environ. Microbiol. 33: 254–256.
De Bont, J. A. M., Scholten, A. and Hansen, Th. A. 1980. DNA-DNA hybridization studies of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides and Rhodopseudomonas sulfidophila strains.—In preparation.
Hungate, R. E. 1950. The anaerobic mesophilic cellulolytic bacteria. — Bacteriol. Rev. 14: 1–50.
Karlsson, J. L., Volcani, B. E. and Barker, H. A. 1948. The nutritional requirements of Clostridium aceticum. — J. Bacteriol. 56: 781–782.
Mulder, E. G., Adamse, A. D., Antheunisse, J., Deinema, M. H., Woldendorp, J. W. and Zevenhuizen, L. P. T. M. 1966. The relationship between Brevibacterium linens and bacteria of the genus Arthrobacter. — J. Appl. Bacteriol. 29: 44–71.
Ohwaki, K. and Hungate, R. E. 1977. Hydrogen utilization by Clostridia in sewage sludge. — Appl. Environ. Microbiol. 33: 1270–1274.
Wieringa, K. T. 1936. Over het verdwijnen van waterstof en koolzuur onder anaërobe voorwaarden. — Antonie van Leeuwenhoek 3: 263–271.
Wieringa, K. T. 1939/40. The formation of acetic acid from carbon dioxide and hydrogen by anaerobic spore-forming bacteria. — Antonie van Leeuwenhoek 6: 251–262.
Zeikus, J. G. 1977. The biology of methanogenic bacteria. — Bacteriol. Rev. 41: 514–541.
Author information
Authors and Affiliations
Additional information
In memory of Dr Klaas Tammo Wieringa (1891–1980), who has dedicated more than fifty years of his life to many aspects of agricultural microbiology and biotechnology in our laboratory.
Rights and permissions
About this article
Cite this article
Adamse, A.D. New isolation of Clostridium aceticum (Wieringa). Antonie van Leeuwenhoek 46, 523–531 (1980). https://doi.org/10.1007/BF00394009
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00394009