Skip to main content
SpringerLink
Log in

Production of polysaccharide hydrolases in the genusRhizopus

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Polysaccharide hydrolase activity was assayed in a group of 28 selectedRhizopus strains. The production of lichenases, mannanases, cellulases, xylanases, amylases and pullulanases was demonstrated using the gel-testing method during growth of the strains on suitably meshed polysaccharide gels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Augustiín J., Šikl D.: α-Mannosidase of generaAspergillus andRhizopus. Activity and capacity to utilizeSaccharomyces cerevisiae mannan of the best α-mannosidase producerAspergillus flavus Link 69.Folia Microbiol. 23, 349–352 (1978).

    CAS  Google Scholar 

  • Augustiín J., Zemek J., Kocková-Kratochvílová A., Kuniak L'.: Production of α-amylase by yeasts and yeast-like organisms.Folia Microbiol. 23, 353–361 (1978).

    CAS  Google Scholar 

  • Augustín J., Zemek J., Kuniak L'., Kocková-Kratochvílová A.: Mannan-hydrolyzing enzymes of yeasts and yeast-like organisms.Folia Microbiol. 25, 301–305 (1980).

    Google Scholar 

  • Augustín J., Zemek J., Fassatiová O., Kuniak L'.: Production of α-amylase by microscopic fungi.Folia Microbiol. 26, 142–146 (1981).

    Google Scholar 

  • Augustín J., Kuniak L'., Páčová Z.: Investigation of the enzymatic equipment of a selected panel of bacterial strains of the genusPseudomonas.Biologia 49, 807–812 (1994).

    Google Scholar 

  • Augustín J., Kuniak L'., Hudecová D.: Screening of yeasts and yeast-like organismsAureobasidium pullulans for pullulan production.Biologia 52, 399–404 (1997).

    Google Scholar 

  • Augustín J.: Enzyme equipment inAureobasidium pullulans.Folia Microbiol. 45, 143–146 (2000).

    Google Scholar 

  • Basri M., Heng A.C., Irazak C.N.A.: Alcoholysis of palm oil mid-fraction by lipase fromRhizopus rhizopodiformis.J. Am. Oil Chem. Soc. 74, 113–116 (1997).

    Article  CAS  Google Scholar 

  • Davídek J., Hrdlička J., Karvánek M., Pokorný J., Seifert J., Velíšek J.:Laboratory Guide of Food Analysis. (In Czech) SNTL Publishers, Prague 1977.

    Google Scholar 

  • Fassatiová O.:Moulds and Filamentous Fungi in Technical Microbiology. (in Czech) SNTL Publishers, Prague 1979.

    Google Scholar 

  • Fujimoto H., Isomura M., Ajisaka K.: Syntheses of alkyl β-d-mannopyranosides and β-1,4 linked oligosaccharides using β-mannosidase fromRhizopus niveus.Biosci. Biotech. Biochem. 61, 164–165 (1997).

    Article  CAS  Google Scholar 

  • Ginterová A., Janotková O., Zemek J., Augustín J., Kuniak L'.: Cellulase activity of higher fungi.Folia Microbiol. 25, 318–323 (1980).

    Google Scholar 

  • Ginterová A., Janotková O., Zemek J., Augustín J., Kuniak L'.: Orientation screening of α-amylolytic activity in higher fungi and comparison with the cellulolytic activity.Biologia 36, 23–29 (1981).

    Google Scholar 

  • Hiromi K., Iwata K., Nishikawa U., Ohnishi M., Tomita T.: Kinetic properties of the amylases,Rhizopus glucoamylases andBacillus α-amylase which are immobilized on cellulofine.Starch 42, 486–489 (1990).

    Article  Google Scholar 

  • Inui T., Takeda Y., Iizika H.: Taxonomical studies of genusRhizopus.Rep. Inst. Appl. Microbiol. Univ. Tokyo 11, 1–14 (1966).

    Google Scholar 

  • James J., Lee B.H.: Glucoamylases microbial sources, industrial application and molecular biology—a review.J. Food Biochem. 21, 1–52 (1997).

    Article  CAS  Google Scholar 

  • Keil B., Šormová Z. (Eds):Laboratory Techniques in Biochemistry. (in Czech) Publishing House of the Czechoslovak Academy of Sciences, Prague 1959.

    Google Scholar 

  • Kocková-Kratochvílová A., Sláviková E., Zemek J., Augustín J., Kuniak L'., Dercová K.: Numerical taxonomy of the genusSchwanniomyces klockeri.Biologia 36, 693–701 (1981).

    Google Scholar 

  • Kunert J., Zemek J., Augustín J., Kuniak L'., Lýsek H.: Polysaccharide-hydrolyzing activity of ovicidal fungi.Biologia 37, 291–299 (1982).

    CAS  Google Scholar 

  • Lukášová J., Zemek J., Augustín J., Kuniak L'.: Production of hydrolases in staphylococci isolated from foodstuffs.Arch. Lebensmittelhyg. 33, 109–136 (1982).

    Google Scholar 

  • Morita H., Fujio Y.: High specific activity of raw starch digesting glucoamylase producingRhizopus sp. A-11 in liquid culture.Starch 49, 293–296 (1997).

    Article  CAS  Google Scholar 

  • Murai T., Ueda M., Shibasak Y.: Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface.Appl. Microbiol. Biotechnol. 51, 65–70 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Oote S., Aikawa T., Takahara J., Gijutsian K.: Cellulase ofRhizopus—I. Determination of cellulase activity and the effect of medium composition on the production of cellulase byRhizopus.Hakko Kengyusho Hokoku 26, 65–72 (1964a).

    Google Scholar 

  • Oote S., Aikawa T., Takahara J., Gijutsian K.: Cellulase ofRhizopus—II. Distribution of cellulase-forming microorganisms inRhizopus genus.Hakko Kengiusho Hokoku 26, 73–79 (1964b).

    Google Scholar 

  • Panov V.P., Kirsanov A.V., Gregorov V.S., Pankratov A.J.: Morphology and physiologyRhizopus delemar.Microbiology 39, 869–871 (1970).

    PubMed  CAS  Google Scholar 

  • Saadat S., Kamisango K., Ballou C.E.: Enzymatic degradation of the micobacterialO-methyl-d-glucose polysaccharide by aRhizopus-mold α-amylase, an enzyme active on 6-O-methyl-amylo-oligosaccharides.Carbohydr. Res. 148, 309–319 (1986).

    Article  PubMed  CAS  Google Scholar 

  • Susumu N.: Purification, crystalization and properties of an endo-β-1,3-glucanase fromRhizopus chinensis.Agric. Biol. Chem. 39, 2163–2169 (1975).

    Google Scholar 

  • Takahasi T., Tsuchida Y., Irie M.: Purification on some properties of three forms of glucoamylase fromRhizopus sp.J. Biochem. 84, 1183–1194 (1978).

    Google Scholar 

  • Tanaka A., Fukuchi Y., Ohnishi M., Hiromi K., Aibara S., Morita Y.: Fractionation of isoenzymes and determination of subsite structure of glucoamylase fromRhizopus niveus.Agric. Biol. Chem. 47, 573–580 (1983).

    CAS  Google Scholar 

  • Veluz G.A., Katoh T., Morita H., Fujio Y.: Screening of xylanase-producingRhizopus spp.J. Fac. Agricult. Kyushu Univ. 43, 419–423 (1997).

    Google Scholar 

  • Vincúrová M., Zemek J., Augustín J., Schwartz E., Kuniak L'.: Hydrolases of polysaccharides in typical and atypical mycobacteria.Stud. Pneumol. Phtiseol. Czechosl. 43, 81–85 (1983).

    Google Scholar 

  • Wells J.M.: Growth of theRhizopus stolonifer in low-oxygen atmosphere and production of pectic and cellulolytic enzymes.Phytopathology 58, 1958–1959 (1968).

    Google Scholar 

  • Zemek, J., Kuniak L'., Augustín J.: A selection method of microorganisms for polysaccharide-hydrolase production.Czechosl. Pat. Appl. PV-4379 (1978).

  • Zemek J., Kuniak L'., Borriss R., Kuniak L'., Švábová M., Páčová Z.: Polysaccharide-hydrolyzing enzymes in the genusBacillus.Folia Microbiol. 26, 403–407 (1981a).

    CAS  Google Scholar 

  • Zemek J., Kuniak L'., Augustín J.: Production of polysaccharide enzymes—a new method for selection of producing strains.Abh. Akad. Wiss. DDR Abt. Math. Naturwiss. Tech. 3, 47–53 (1981b).

    Google Scholar 

  • Zouchová Z., Kocourek J., Musílek V.: α-Mannosidase and mannanase of some wood-roting fungi.Folia Microbiol. 26, 61–65 (1977).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, 809 33, Bratislava, Slovakia

    N. Kolarova

  2. Department of Milk, Fats and Food Hygiene, Faculty of Chemical and Food Technology, Slovak Technical University, 812 37, Bratislava, Slovakia

    J. Augustín

Authors
  1. N. Kolarova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Augustín
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Augustín.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kolarova, N., Augustín, J. Production of polysaccharide hydrolases in the genusRhizopus . Folia Microbiol 46, 223–226 (2001). https://doi.org/10.1007/BF02818537

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02818537

Keywords

Search

Navigation