Abstract
The pretreatment of softwood with sulfuric acid impregnation in the production of ethanol, based on enzymatic hydrolysis, has been investigated. The parameters investigated were: H2SO4 concentration (0.5 – 4.4% w/w liquid), temperature (180 – 240°C), and residence time (1-20 minutes). The combined severity (log Ro-pH) was used to combine the parameters into a single reaction ordinate. The highest yields of fermentable sugars, i.e., glucose and mannose, were obtained at a combined severity of 3. At this severity, however, the fermentability declined and the ethanol yield decreased. In a comparison with previous results, SO2 impregnation was found to be preferable, since it resulted in approximately the same sugar yields, but better fermentability.
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Torget, R., Werdene, P., Himmel, M., and Grohmann, K. (1990),Appl. Biochem. Biotechnol. 24/25, 115–126.
Martinez, J., Negro, M. J., Saez, F., Manero, J., Saez, R., and Martin, C. (1990),Appl. Biochem. Biotechnol. 24/25, 127–134.
Ropars, M., Marchal, R., Pourquié, J., and Vandecasteele, J. P. (1992)Bioresource. Technol. 42, 197–204.
Grethlein, H. E. and Converse, A. O. (1991),Bioresource Technol. 36, 77–82.
Ramos, L. P., Breuil, C, and Saddler, J. N. (1992),Appl. Biochem. Biotechnol. 34/35, 37–48.
Gregg, D. J. and Saddler, J. N. (1996),Biotechnol. Bioeng. 51, 375–383.
Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985),J. Wood Chem. Technol. 5, 405–425.
Schwald, W., Breuil, C., Brownell, H. H., Chan M., and Saddler, J. N. (1989),Appl. Biochem. Biotechnol. 20/21, 29–44.
Schwald, W., Smaridge, T., Chan, M., Breuil, C, and Saddler, J. N. (1989), inEnzyme Systems for Lignocellulose Degradation, Coughlan, M. P., ed., Elsevier, New York, pp. 231–242.
Mamers, H. and Menz, D. N. J. (1984),Appita 37, 644–649.
Clark, T. A., Mackie, K. L., Dare, P. H., and McDonald, A. G. (1989),J. Wood Chem. Technol. 9 135–166.
Clark, T. A. and Mackie, K. L. (1987),J. Wood Chem. Technol. 7, 373–403.
Stenberg, K., Tengborg, C., Galbe, M., and Zacchi, G. (1997)J. Chem. Tech. Biotechnol, accepted.
von Sivers, M. and Zacchi, G. (1996),Bioresource. Technol. 56, 131–140.
Overend, R. P. and Chornet, E. (1987),Phil. Trans. R. Soc. London, Series A,321, 523–536.
Chum, H. L., Johnson, D. K., Black, S. K., and Overend, R. P. (1990),Appl. Biochem. Biotechnol. 24/25, 1–14.
Chum, H. L., Johnson, D. K., and Black, S. K. (1990),Ind. Eng. Chem. Res. 29, 156–162.
van Walsum, G. P., Allen, S. G., Spencer, M. J., Laser, M. S., Antal, M. J., and Lynd, L. R. (1996),Appl. Biochem. Biotechnol. 57/58, 157–170.
Mandels, M., Andreotti, R., and Roche, C. (1976),Biotechnol. Bioeng. Symp. 6, 21–33.
Berghem, L. E. R. and Petterson, L. G. (1974), Eur. J. Biochem.46, 295–305.
Eklund, R., Galbe, M., and Zacchi G. (1995),Bioresource. Eng. 52, 225–229.
Stenberg, K., Tengborg, C, Galbe, M., Zacchi, G., Palmqvist, E., and Hahn-Hagerdal, B., (1997),Appl. Biochem. Biotechnol., accepted.
Nilvebrant, N.-O., Reimann, A., de Sousa, F., Kleen, M., and Palmqvist, E. (1997) Proc. 9th International Symposium on Wood and Pulping Chemistry, Poster no. 79, Montréal, Canada, June 9 – 12.
Baugh, K. D. and McCarty, P. L. (1988),Biotechnol Bioeng. 31, 50–61.
Clark, T. A. and Clark, T. A. (1984),J. Chem. Tech. Biotechnol. 34B, 101–110.
Russel, N. J. and Gould, G. W. (1991),Food Preservatives, Blackie and Son, Glasgow and London.
Taherzadeh, M. J., Niklasson, C., and Lidén, G. (1997),Chem. Eng. Sci. 52, 2653–2659.
Torget, R., Hatzis, C., Hayward, T. K., Hsu, T.-A., and Philippidis, G. P. (1996),Appl. Biochem. Biotechnol. 57/58, 85–101.
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Tengborg, C., Stenberg, K., Galbe, M. et al. Comparison of SO2 and H2SO4 impregnation of softwood prior to steam pretreatment on ethanol production. Appl Biochem Biotechnol 70, 3–15 (1998). https://doi.org/10.1007/BF02920119
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DOI: https://doi.org/10.1007/BF02920119