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Effects of mixing conditions on the production of microbial cellulose byAcetobacter xylinum

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Abstract

Microbial cellulose has many potential applications due to its excellent physical properties. The production of cellulose fromAcetobacter xylinum in submerged culture is, however, beset with numerous problems. The most difficult one has been the appearance of negative mutants under shaking culture conditions, which is deficient of cellulose producing ability. Thus genetic instability ofAcetobacter xylinum under shaking culture condition made developing a stable mutant major research interest in recent years. To find a proper type of bioreactor for the production of microbial cellulose, several production systems were developed. Using a reactor system with planar type impeller with bottom sparging system, it was possible to produce 5 g/L microbial cellulose without generating cellulose minus mutants, which is comparable to that of static culture system.

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  1. Division of Chemical Engineering, College of Engineering, Sun Moon University, Asan, 336-840, Chungnam, Korea

    Hei Chan Lee & Xia Zhao

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  1. Hei Chan Lee
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  2. Xia Zhao
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Correspondence to Hei Chan Lee.

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Lee, H.C., Zhao, X. Effects of mixing conditions on the production of microbial cellulose byAcetobacter xylinum . Biotechnol. Bioprocess Eng. 4, 41–45 (1999). https://doi.org/10.1007/BF02931912

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