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Isolation and characterization of mesophilic, oxalate-degrading Streptomyces from plant rhizosphere and forest soils

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Abstract

The present work was aimed at the isolation of additional new pure cultures of oxalate-degrading Streptomyces and its preliminary characterization for further work in the field of oxalate metabolism and taxonomic studies. Mesophilic, oxalate-degrading Streptomyces were enriched and isolated from plant rhizosphere and forest soil samples. Strains were examined for cultural, morphological (spore chain morphology, spore mass colour, diffusible and melanin pigment production), physiological (antibiosis, growth in the presence of inhibitory compounds, assimilation of organic acids and enzyme substrates) and chemotaxonomic characters (cellular lipid components and diagnostic cell-wall diamino acid). The taxonomic data obtained were analysed by using the simple matching (SSM) and Jaccard (SJ) coefficients, clustering was achieved using the UPGMA algorithm. All strains were able to utilize sodium-, potassium-, calcium- and ammonium-oxalate salts. Based on the results of numerical taxonomy, isolates were grouped into five cluster groups with a ≥70% SSM similarity level. Streptomyces rochei was the most common of the cluster groups, with a Willcox probability of P>0.8. Streptomyces antibioticus, S. anulatus, S. fulvissimus, S. halstedii and S. violaceusniger are newly reported as oxalate-utilizing Streptomyces.

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Acknowledgements

The present research was partly benefited by a research award from Mugla University Sıtkı Kocman Foundation. The author deeply indebted to three anonymous reviewers for their many helpful comments and suggestions, which improved the first version of this manuscript.

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  1. Egitim Fakultesi, Ortaogretim Fen ve Matematik Alanlar Egitimi Bolumu, Biyoloji Egitimi Anabilim Dalı, Mugla Universitesi, 48170, Kötekli, Mugla, Turkey

    Nurettin Sahin

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Correspondence to Nurettin Sahin.

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Sahin, N. Isolation and characterization of mesophilic, oxalate-degrading Streptomyces from plant rhizosphere and forest soils. Naturwissenschaften 91, 498–502 (2004). https://doi.org/10.1007/s00114-004-0562-4

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