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Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants

  • Experimental Study
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

Purpose

The purpose of this study was to investigate the influence of continuous perfusion and mechanical stimulation on bone marrow stromal cells seeded on a collagen meniscus implant.

Methods

Bone marrow aspirates from 6 donors were amplified in vitro. 106 human BMSC were distributed on a collagen meniscus implant. Scaffolds were cultured under static conditions (control) or placed into a bioreactor system where continuous perfusion (10 ml/min) or perfusion and mechanical stimulation (8 h of 10% cyclic compression at 0.5 Hz) were administered daily. After 24 h, 7 and 14 days, cell proliferation, synthesis of procollagen I and III peptide (PIP, PIIIP), histology, and the equilibrium modulus of the constructs were analyzed.

Results

Proliferation demonstrated a significant increase over time in all groups (p < 0.001). PIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.0 ± 0.5 (perfusion), 3.8 ± 0.3 (mechanical stimulation), and 1.8 ± 0.2 U/ml/g protein (static control, lower than perfusion and mechanical stimulation, p < 0.05). These differences were also evident after 2 weeks (2.7 ± 0.3, 4.0 ± 0.6, and 1.8 ± 0.2 U/ml/g protein, p < 0.01); PIIIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.9 ± 0.7 (perfusion), 3.1 ± 0.9 (mechanical stimulation), and 1.6 ± 0.3 U/ml/g protein (controls) after 1 week and remained significantly elevated under the influence of perfusion and mechanical stimulation (p < 0.01) after 2 weeks. Mechanical stimulation increased the equilibrium modulus more than static culture and perfusion after 2 weeks (24.7 ± 7.6; 12.3 ± 3.7; 15.4 ± 2.6 kPa; p < 0.02).

Conclusion

Biomechanical stimulation and perfusion have impact on collagen scaffolds seeded with BMSCs. Cell proliferation can be enhanced using continuous perfusion and differentiation is fostered by mechanical stimulation.

Level of evidence

Non-applicable experimental study.

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Acknowledgments

We wish to thank J. Viering and H. Schumann for the construction of the bioreactor system used in this study. We acknowledge the writing assistance of Gavin Olender, MSc. We are grateful for the financial support of the “Deutsche Arthrosehilfe” and the German Society of Orthopaedic Traumatologic Sports Medicine (GOTS). We gratefully acknowledge the support of W. Rodkey and Regen Biologics Inc. for this study.

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Authors and Affiliations

  1. Trauma Department, Hanover Medical School (MHH), OE 6230, Carl-Neuberg-Straße 1, 30625, Hannover, Germany

    M. Petri, K. Ufer, I. Toma, E. Liodakis, S. Brand, P. Haas, C. Liu, C. Haasper & M. Jagodzinski

  2. Laboratory for Biomaterials and Biomechanics (LBB), Orthopaedic Department, Hanover Medical School (MHH), Anna-von-Borries-Str. 1–7, 30625, Hannover, Germany

    B. Richter

  3. Orthopaedic Department, Hanover Medical School (MHH), Anna-von-Borries-Str. 1–7, 30625, Hannover, Germany

    C. Becher & G. von Lewinski

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  1. M. Petri
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Petri, M., Ufer, K., Toma, I. et al. Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants. Knee Surg Sports Traumatol Arthrosc 20, 223–231 (2012). https://doi.org/10.1007/s00167-011-1600-3

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  • DOI: https://doi.org/10.1007/s00167-011-1600-3

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