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Enhanced synthesis and antimicrobial activities of bacteriocins produced by Mexican strains of Bacillus thuringiensis

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Abstract

Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial activity, being toxic to clinically significant against Gram-positive and Gram-negative bacteria, including common etiological agents of human diseases, such as strep throat and scarlet fever, septicemia, pneumonia, urinary tract infection, and emetic and gastrointestinal syndromes. Although synthesis of the five Bt-BLIS was independent of the presence of a target inducing bacterium, we demonstrated for the first time that a proteinaceous component(s) secreted by, or liberated by proteolytic cleavage of Bacillus cereus 183 following treatment with proteinase K, enhanced Bt-BLIS synthesis.

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Acknowledgments

We thank Gabriela Morales-Pérez and Roberto Ortega Monzon, undergraduate students from Universidad de Guanajuato, Mexico, and Janeth Adriana Martínez-Cardeñas from Universidad Juarez Autonoma de Tabasco, Mexico, for their technical assistance. Norma de la Fuente-Salcido is a graduate student supported by a fellowship sponsored by CONACYT-México. The authors are grateful for the essential material provided by Jorge Ibarra, Irais Sánches-Ortega and Ma. Alejandra Chavira-Zuñiga. This research was supported partially by a grant (DINPO 2006) from the Universidad de Guanajuato to JEB-C.

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Authors and Affiliations

  1. Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Instituto de Ciencias Agrícolas, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico

    N. de la Fuente-Salcido, R. Salcedo-Hernández, M. Bautista-Justo & J. E. Barboza-Corona

  2. Universidad Autónoma de Coahuila, Escuela de Ciencias Biológicas, 27440, Torreon, Coahuila, Mexico

    N. de la Fuente-Salcido

  3. Universidad Autónoma de Nuevo Léon. Facultad de Ciencias Biológicas, San Nicolás de los Garza, Nuevo Leon, Mexico

    N. de la Fuente-Salcido & Ma. Guadalupe Alanís-Guzmán

  4. Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, CA, 92504, USA

    D. K. Bideshi

  5. Department of Entomology, University of California, Riverside, CA, 92521, USA

    D. K. Bideshi

Authors
  1. N. de la Fuente-Salcido
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  2. Ma. Guadalupe Alanís-Guzmán
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  3. D. K. Bideshi
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  4. R. Salcedo-Hernández
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  5. M. Bautista-Justo
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  6. J. E. Barboza-Corona
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Corresponding author

Correspondence to J. E. Barboza-Corona.

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Communicated by Erko Stackebrandt.

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de la Fuente-Salcido, N., Guadalupe Alanís-Guzmán, M., Bideshi, D.K. et al. Enhanced synthesis and antimicrobial activities of bacteriocins produced by Mexican strains of Bacillus thuringiensis . Arch Microbiol 190, 633–640 (2008). https://doi.org/10.1007/s00203-008-0414-2

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  • DOI: https://doi.org/10.1007/s00203-008-0414-2

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