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Dactylorhin B reduces toxic effects of β-amyloid fragment (25–35) on neuron cells and isolated rat brain mitochondria

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Abstract

β-amyloid is strongly implicated in Alzheimer’s pathology, and mitochondria play an important role in neurodegenerative disorders. Dactylorhin B [short for bis(4-β-D-glucopyranosyloxybenzyl)-2-β-D-glucopyranosyl-2-isobutyltartrate (DHB)] is an active compound isolated from Coeloglossum viride. (L.) Hartm. var. bracteatum (Willd.) and may have neuroprotective effects. In the present study, we investigated the damage of rat brain mitochondrial function induced by β-amyloid and the protective effect of DHB. Isolated rat brain mitochondria were incubated with amyloid-β peptide (Aβ)25–35 alone or together with DHB. reactive oxygen species production, pyruvate dehydrogenase complex activity, α-ketoglutarate dehydrogenase complex activity, cytochrome c oxidase activity, mitochondrial swelling, mitochondrial membrane potential and the release of cytochrome c from mitochondria were measured. Data showed that Aβ25–35 directly disrupted mitochondrial function, inhibited the key enzymes and contributed to apoptosis and the deficiency of energy metabolism. Co-incubation of DHB attenuated Aβ25–35-induced pathological changes. The significance of DHB in the management of mitochondria-related disorders is discussed.

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Acknowledgments

This work was supported by research grants from the Natural Science Foundation of China (30472179) and the National High-tech R&D Program (863 Program) (2004AA2Z3815). We thank Dr. Xiaoliang Wang for reading the manuscript and providing helpful comments and suggestions.

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Authors and Affiliations

  1. Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing, 100050, China

    Dan Zhang, Yi Zhang, Gengtao Liu & Jianjun Zhang

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  1. Dan Zhang
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  2. Yi Zhang
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Correspondence to Jianjun Zhang.

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Zhang, D., Zhang, Y., Liu, G. et al. Dactylorhin B reduces toxic effects of β-amyloid fragment (25–35) on neuron cells and isolated rat brain mitochondria. Naunyn-Schmied Arch Pharmacol 374, 117–125 (2006). https://doi.org/10.1007/s00210-006-0095-9

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