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Preliminary characterization of a light-rare-earth-element-binding peptide of a natural perennial fern Dicranopteris dichotoma

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Abstract

A light-rare-earth-element (LREE)-binding peptide was isolated from LREE hyperaccumulator Dicranopteris dichotoma leaves and characterized in terms of molecular weight and ultraviolet absorption spectrum. The molecular weight of the LREE-binding peptide was determined to be 2208 Da by matrix-assisted laser-desorption ionization–time of flight mass spectrometry (MALDI–TOFMS). The characteristic ultraviolet absorption spectrum of the peptide was observed at 220–300 nm, suggesting that the peptide chain contained aromatic amino acids. Compared to the unique features of the phytochelatins with a low absorption at 280 nm, the LREE-binding peptide is unlikely to be a typical phytochelatin. The present study suggests that the LREE-binding peptide is probably a natural peptide in D. dichotoma, and it may play an important role in hyperaccumulation of LREEs.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 20237010, 20177030 and 40171086).

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Authors and Affiliations

  1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, 100085, Beijing, China

    Haiou Wang, Xiao-quan Shan, Shuzhen Zhang & Bei Wen

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  1. Haiou Wang
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  2. Xiao-quan Shan
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  3. Shuzhen Zhang
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  4. Bei Wen
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Correspondence to Xiao-quan Shan.

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Wang, H., Shan, Xq., Zhang, S. et al. Preliminary characterization of a light-rare-earth-element-binding peptide of a natural perennial fern Dicranopteris dichotoma . Anal Bioanal Chem 376, 49–52 (2003). https://doi.org/10.1007/s00216-003-1853-x

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  • DOI: https://doi.org/10.1007/s00216-003-1853-x

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