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Effect-directed analysis: a promising tool for the identification of organic toxicants in complex mixtures?

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Abstract

Wastewater effluents, groundwater, surface water, sediments, soils and air particulate matter are often contaminated by a multitude of chemicals. Since often no a priori knowledge of relevant toxicants exists, chemical analysis alone is not an appropriate tool for hazard assessment. Instead, a linkage of effect data and hazardous compounds is required. For that purpose, effect-directed analysis (EDA) was developed, which is based on a combination of biotesting, fractionation procedures and chemical analytical methods. Since a controversial discussion about the prospects of success in relation to the expense exists, the current methodological state of EDA for organic toxicants in complex mixtures and important results are reviewed in this paper with the aim of establishing criteria for the successful use of this promising tool. While EDA is a powerful tool to identify specifically acting individual toxicants close to the source of emission, it is inappropriate for screening purposes and often may fail in remote areas where the concentrations of specific toxicants are too low relative to the nonspecific toxicity of the whole mixture of natural and anthropogenic compounds. The biological tools have to be carefully selected with respect to their ability to detect specific effects and their significance in hazard assessment. Sophisticated chemical tools are required to identify individual toxicants in mixtures of thousands of compounds, which are typical for contaminated environments.

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References

  1. Ankley GT, Mount DR (1996) Human Ecol Risk Assess 2:434–440

    Google Scholar 

  2. Jacobs MW, Coates JA, Delfino JJ, Bitton G, Davis WM, Garcia KL (1993) Arch Environ Contam Toxicol 24:461–468

    CAS  Google Scholar 

  3. Samoiloff MR, Bell J, Birkholz DA, Webster GRB, Arnott EG, Pulak R, Madrid A (1983) Environ Sci Technol 17:329–334

    CAS  Google Scholar 

  4. Reemtsma T, Fiehn O, Jekel M (1999) Fresen J Anal Chem 363:771–776

    Article  CAS  Google Scholar 

  5. Schuetzle D, Lewtas J (1986) Anal Chem 58:1060A–1075A

    PubMed  Google Scholar 

  6. Reemtsma T (2001) Anal Chim Acta 426:279–287

    Article  CAS  Google Scholar 

  7. Norberg-King TJ, Mount DI, Durhan EJ, Ankley GT, Burkhard LP, Amato JR, Lukasewycz MT, Schubauer-Berigan MK, Anderson-Carnahan L (1991) Methods for aquatic toxicity identification evaluation. Phase I toxicity characterization procedures. US Environmental Protection Agency, EPA/600/6–91/003

  8. Mount DI, Anderson-Carnahan L (1989) Methods for aquatic toxicity identification evaluation. Phase II toxicity identification procedures. US Environmental Protection Agency, EPA/600/3–88/035

  9. Mount DI (1989) Methods for aquatic toxicity identification evaluations. Phase III toxicity confirmation procedures. US Environmental Protection Agency, EPA/600/3–88/036

  10. Burgess RM (2000) Int J Environ Pollut 13:2–33

    CAS  Google Scholar 

  11. Ho KT, Burgess RM, Pelletier MC, Serbst JR, Ryba SA, Cantwell MG, Kuhn A, Raczelowski P (2002) Mar Pollut Bull 44:286–293

    Article  CAS  PubMed  Google Scholar 

  12. Castillo M, Barcelo D (2001) Anal Chim Acta 426:253–264

    CAS  Google Scholar 

  13. Holmborn B, Voss RH, Mortimer RD, Wong A (1984) Environ Sci Technol 18:333–337

    Google Scholar 

  14. Burkhard LP, Durhan EJ, Lukasewycz MT (1991) Anal Chem 63:277–283

    CAS  Google Scholar 

  15. Amato JR, Mount DI, Durhan EJ, Lukasewycz MT, Ankley GT, Robert ED (1992) Environ Toxicol Chem 11:209–216

    CAS  Google Scholar 

  16. Jop KM, Kendall TZ, Askew AM, Foster RB (1991) Environ Toxicol Chem 10:981–990

    CAS  Google Scholar 

  17. Huckins JN, Tubergen MW, Manuweera GK (1990) Chemosphere 20:533–552

    CAS  Google Scholar 

  18. Huckins JN, Manuweera GK, Petty JD, Mackay D, Lebo JA (1993) Environ Sci Technol 27:2489–2496

    CAS  Google Scholar 

  19. Vrana B, Paschke A, Popp P, Schüürmann G (2001) Environ Sci Pollut Res 8:27–34

    CAS  Google Scholar 

  20. Sabaliunas D, Ellington J, Sabaliuniene I (1999) Ecotox Environ Saf 44:160–167

    Article  CAS  Google Scholar 

  21. Fiehn O, Jekel M (1996) Anal Chem 68:3083–3089

    Article  CAS  Google Scholar 

  22. Fiehn O, Vigelahn L, Kalnowski G, Reemtsma T, Jekel M (1997) Acta Hydrochim Hydrobiol 25:11–16

    CAS  Google Scholar 

  23. Hayatsu H (1992) J Chrom 597:37–56

    Article  CAS  Google Scholar 

  24. Sayato Y, Nakamuro K, Ueno H, Goto R (1993) Mut Res 300:207–213

    Article  CAS  Google Scholar 

  25. Nukaya H, Yamashita J, Tsuji K, Terao Y, Ohe T, Sawanishi H, Katsuhara T, Kiyokawa K, Tezuka M, Oguri A, Sugimura T, Wakabayashi K (1997) Chem Res Toxicol 10:1061–1066

    Article  CAS  PubMed  Google Scholar 

  26. Oguri A, Shiozawa T, Terao Y, Nukaya H, Yamashita J, Ohe T, Sawanishi H, Katsuhara T, Sugimura T, Wakabayashi K (1998) Chem Res Toxicol 11:1195–1200

    Article  CAS  PubMed  Google Scholar 

  27. Sousa S, Duffy C, Weitz H, Glover LA, Bär E, Henkler R, Killham K (1998) Environ Toxicol Chem 17:1039–1045

    CAS  Google Scholar 

  28. Brack W, Rottler H, Frank H (1998) Environ Toxicol Chem 17:1982–1991

    CAS  Google Scholar 

  29. Brack W, Frank H (1997) Chemosphere 34:849–854

    Article  CAS  Google Scholar 

  30. Brack W, Altenburger R, Ensenbach U, Möder M, Segner H, Schüürmann G (1999) Arch Environ Contam Toxicol 37:164–174

    Article  CAS  PubMed  Google Scholar 

  31. Brack W, Schirmer K, Kind T, Schrader S, Schüürmann G (2002) Environ Toxicol Chem 21:2654–2662

    CAS  PubMed  Google Scholar 

  32. DeMarini DM, Williams RW, Perry E, Lemieux PM, Linak WP (1992) Combust Sci Technol 85:437–453

    CAS  Google Scholar 

  33. Dobias L, Kusova J, Gajdos O, Vidova P, Gajdosova D, Havrankova J, Fried M, Binkova B, Topinka J (1999) Mut Res 445:285–293

    CAS  Google Scholar 

  34. Fernandez P, Grifoll M, Solanas AM, Bayona JM, Albaiges J (1992) Environ Sci Technol 26:817–829

    CAS  Google Scholar 

  35. Brooks LR, Hughes TJ, Claxton LD, Austern B, Brenner R, Kremer F (1998) Environ Health Persp 106, Supplement 6:1435–1440

    Google Scholar 

  36. Thomas KV, Balaam J, Barnard N, Dyer R, Jones C, Lavender J, McHugh M (2002) Chemosphere 49:247–258

    Article  PubMed  Google Scholar 

  37. Salmeen IT, Pero AM, Zator R, Schuetzle D, Riley TL (1984) Environ Sci Technol 18:375–382

    CAS  Google Scholar 

  38. Lewtas J, King LC, Williams K, Ball LM, DeMarini DM (1990) Mutagenesis 5:481–489

    CAS  PubMed  Google Scholar 

  39. DeMarini DM, Lemieux PM, Ryan JV, Brooks LR, Williams RW (1994) Environ Sci Technol 28:136–141

    CAS  Google Scholar 

  40. Durant JL, Lafleur AL, Plummer EF, Taghizadeh K, Busby WF, Thilly WG (1998) Environ Sci Technol 32:1894–1906

    Article  CAS  Google Scholar 

  41. Marvin CH, Allan L, McCarry BE, Bryant DW (1993) Environ Mol Mutagen 22:61–70

    CAS  Google Scholar 

  42. Marvin CH, McCarry BE, Villella J, Allan LM, Bryant DW (2000) Chemosphere 41:989–999

    Article  CAS  PubMed  Google Scholar 

  43. Nishioka MG, Howard CG, Contos DA, Ball LM, Lewtas J (1988) Environ Sci Technol 22:908–915

    CAS  Google Scholar 

  44. Spinosa De Martinis BS, Kado NY, de Carvalho LRF, Okamoto RA, Gundel LA (1999) Mut Res 446:83–94

    Google Scholar 

  45. Durant JL, Hemond HF, Thilly W (1992) Environ Sci Technol 26:599–608

    CAS  Google Scholar 

  46. Durant JL, Monchamp PA, Lafleur AL, Hemond HF (1994) Environ Sci Technol 28:1819–1828

    CAS  Google Scholar 

  47. Marvin CH, Tessaro M, McCarry BE, Bryant DW (1994) Sci Tot Environ 156:119–131

    CAS  Google Scholar 

  48. Marvin CH, Lundrigan JA, McCarry BE, Bryant DW (1995) Environ Toxicol Chem 14:2059–2066

    CAS  Google Scholar 

  49. Marvin CH, Allan L, McCarry BE (1992) Intern J Environ Anal Chem 49:221–230

    CAS  Google Scholar 

  50. Brunström B, Broman D, Dencker L, Näf C, Vejlens E, Zebühr Y (1992) Environ Toxicol Chem 11:1441–1449

    Google Scholar 

  51. Engwall M, Brunström B, Brewer A, Norrgren L (1994) Aquat Toxicol 30:311–324

    Article  CAS  Google Scholar 

  52. Engwall M, Broman D, Ishaq R, Näf C, Zebühr Y, Brunström B (1996) Environ Toxicol Chem 15:213–222

    CAS  Google Scholar 

  53. Engwall M, Broman D, Dencker L, Näf C, Zebühr Y, Brunström B (1997) Environ Toxicol Chem 16:1187–1194

    CAS  Google Scholar 

  54. Wenzel K-D, Hubert A, Manz M, Weissflog L, Engewald W, Schüürmann G (1998) Anal Chem 70:4827–4835

    CAS  Google Scholar 

  55. Hubert A, Wenzel K-D, Manz M, Weissflog L, Engewald W, Schüürmann G (2000) Anal Chem 72:1294–1300

    Article  CAS  PubMed  Google Scholar 

  56. Brack W, Kind T, Hollert H, Schrader S, Möder M (2003) J Chrom A 986:55–66

    Article  Google Scholar 

  57. Lukasewycz M, Durhan E (1992) J Chrom 580:215–228

    Article  CAS  Google Scholar 

  58. Thomas KV, Thain JE, Waldock MJ (1999) Environ Toxicol Chem 18:401–411

    CAS  Google Scholar 

  59. Thomas KV, Hurst MR, Matthiessen P, Sheahan D, Williams RJ (2001) Water Res 35:2411–2416

    Article  CAS  PubMed  Google Scholar 

  60. Thomas KV, Benstead RE, Thain JE, Waldock MJ (1999) Mar Pollut Bull 38:925–932

    Article  CAS  Google Scholar 

  61. Thomas KV, Hurst MR, Matthiessen P, McHugh M, Smith A, Waldock MJ (2002) Environ Toxicol Chem 21:1456–1461

    CAS  PubMed  Google Scholar 

  62. Thomas KV, Hurst MR, Matthiessen P, Waldock MJ (2001) Environ Toxicol Chem 20:2165–2170

    CAS  PubMed  Google Scholar 

  63. Snyder SA, Villeneuve DL, Snyder EM, Giesy JP (2001) Environ Sci Technol 35:3620–3625

    Article  CAS  PubMed  Google Scholar 

  64. Svenson A, Norin H, Hynning PA (1996) Environ Toxicol Wat Qual 11:277–284

    Article  CAS  Google Scholar 

  65. Galassi S, Benfenati E (2000) J Chrom A 889:149–154

    Article  CAS  Google Scholar 

  66. Castano A, Vega M, Blazquez T, Tarazona JV (1994) Environ Toxicol Chem 13:1607–1611

    CAS  Google Scholar 

  67. Vega MM, Fernandez C, Blazquez T, Tarazona JV, Castano A (1996) Environ Pollut 93:135–139

    Article  CAS  Google Scholar 

  68. Bobeldijk I, Brandt A, Wullings B, Noij T (2001) J Chrom A 918:277–291

    Article  CAS  Google Scholar 

  69. Snyder SA, Keith TL, Verbrugge DA, Snyder EM, Gross TS, Kannan K, Giesy JP (1999) Environ Sci Technol 33:2814–2820

    CAS  Google Scholar 

  70. Eberz G, Rast H-G, Burger K, Kreiss W, Weisemann C (1996) Chromatographia 43:5–9

    CAS  Google Scholar 

  71. Reemtsma T, Putschew A, Jekel M (1999) Waste Manage 19:181–188

    Article  CAS  Google Scholar 

  72. Higashi RM, Cherr GN, Shenker JM, Macdonald JM, Crosby DG (1992) Environ Sci Technol 26:2413–2420

    CAS  Google Scholar 

  73. Grifoll M, Solanas AM, Bayona JM (1990) Arch Environ Contam Toxicol 19:175–184

    CAS  PubMed  Google Scholar 

  74. Balch GC, Metcalfe CD, Huestis SY (1995) Environ Toxicol Chem 14:79–91

    CAS  Google Scholar 

  75. Legzdins AE, McCarry BE, Marvin CH, Bryant DW (1995) Int J Environ Anal Chem 60:79–94

    CAS  Google Scholar 

  76. Møller M, Alfheim I, Larssen S, Mikalsen A (1982) Environ Sci Technol 16:221–225

    Google Scholar 

  77. Ho KTY, Quinn JG (1993) Environ Toxicol Chem 12:823–830

    CAS  Google Scholar 

  78. Alfheim I, Löfroth G, Moller M (1983) Environ Health Persp 47:227–238

    CAS  Google Scholar 

  79. DeMarini DM, Williams RW, Brooks LR, Taylor MS (1992) Intern J Environ Anal Chem 48:187–199

    CAS  Google Scholar 

  80. Durant JL, Thilly WG, Hemond HF, Lafleur AL (1994) Environ Sci Technol 28:2033–2044

    CAS  Google Scholar 

  81. Wesp HF, Tang X, Edenharder R (2000) Mut Res 472:1–21

    Article  CAS  Google Scholar 

  82. Bell DA, Karam H, Kamens RM (1990) Environ Sci Technol 24:1261–1264

    CAS  Google Scholar 

  83. DeMarini DM, Shelton ML, Bell DA (1996) Mut Res 349:1–20

    Article  CAS  Google Scholar 

  84. Fernandez P, Bayona JM (1992) J Chrom 625:141–149

    Article  CAS  Google Scholar 

  85. Hannigan MP, Cass GR, Penman BW, Crespi CL, Lafleur AL, Busby WF, Thilly WG, Simoneit BR (1998) Environ Sci Technol 32:3502–3514

    Article  CAS  Google Scholar 

  86. Brack W, Schirmer K (2003) Environ Sci Technol 37 (in press)

  87. Mast TJ, Hsieh DPH, Seiber JN (1984) Environ Sci Technol 18:338–348

    CAS  Google Scholar 

  88. Zebühr Y, Näf C, Bandh C, Broman D, Ishaq R, Pettersen H (1993) Chemosphere 27:1211–1219

    Google Scholar 

  89. Feltz KP, Tillitt D, Gale RW, Peterman PH (1995) Environ Sci Technol 29:709–718

    CAS  Google Scholar 

  90. Gale RW, Long ER, Schwartz TR, Tillitt DE (2000) Environ Toxicol Chem 19:1348–1359

    CAS  Google Scholar 

  91. Castillo M, Barcelo D (1999) Anal Chem 71:3769–3776

    CAS  PubMed  Google Scholar 

  92. Castillo M, Alonso MC, Riu J, Reinke M, Klöter G, Dizer H, Fischer B, Hansen PD, Barcelo D (2001) Anal Chim Acta 426:265–277

    Article  CAS  Google Scholar 

  93. Reineke N, Bester K, Hühnerfuss H, Jastorff B, Weigel S (2002) Chemosphere 47:717–723

    Article  CAS  PubMed  Google Scholar 

  94. Hermens J, Busser F, Leeuwangh P, Musch A (1985) Ecotox Environ Saf 9:17–25

    CAS  Google Scholar 

  95. Schultz TW, Cronin MTD (1997) Environ Toxicol Chem 16:357–360

    CAS  Google Scholar 

  96. Cronin MTD, Schultz TW (1998) Ecotox Environ Saf 39:65–69

    Article  CAS  Google Scholar 

  97. Kaiser KLE, Ribo JM (1988) Tox Assess 3:195–337

    CAS  Google Scholar 

  98. Backhaus T, Froehner K, Altenburger R, Grimme LH (1997) Chemosphere 35:2925–2938

    Google Scholar 

  99. Jacobs MW, Delfino JJ, Bitton G (1992) Environ Toxicol Chem 11:1137–1143

    CAS  Google Scholar 

  100. Gagne F, Pardos M, Blaise C, Turcotte P, Quemerais B, Fouquet A (1999) Chemosphere 39:1545–1570

    Article  CAS  PubMed  Google Scholar 

  101. Salizzato M, Rigoni M, Pavoni B, Volpi Ghirardini A, Ghetti PF (1997) Toxicol Environ Chem 60:183–200

    CAS  Google Scholar 

  102. Salizzato M, Pavoni B (1998) Chemosphere 36:2949–2968

    Article  CAS  Google Scholar 

  103. Pardos M, Bennighoff C, Thomas RL, Kim-Heang S (1999) Environ Toxicol Chem 18:188–193

    CAS  Google Scholar 

  104. Bombardier M, Bermingham N (1999) Environ Toxicol Chem 18:685–698

    CAS  Google Scholar 

  105. Mazidji CN, Koopman B, Bitton G, Voiland G, Logue C (1990) Tox Assess 5:265–277

    CAS  Google Scholar 

  106. Fort DJ, Rogers RL, Copley HF, Bruning LA, Stover EL, Helgen JC, Burkhart JG (1999) Environ Toxicol Chem 18:2316–2324

    CAS  Google Scholar 

  107. Ishaq R, Akerman G, Näf C, Balk L, Bandh C, Broman D (1999) Environ Toxicol Chem 18:533–543

    CAS  Google Scholar 

  108. Harris GE, Metcalfe TL, Metcalfe CD, Huestis SY (1994) Environ Toxicol Chem 13:1393–1403

    CAS  Google Scholar 

  109. Ames BN, McCann J, Yamasaki E (1975) Mut Res 31:347–364

    Article  CAS  Google Scholar 

  110. Mortelmans K, Zeiger E (2000) Mut Res 455:29–60

    Article  CAS  Google Scholar 

  111. Bobeldijk I, Stoks PGM, Vissers JPC, Emke E, van Leerdam JA, Muilwijk B, Berbee R, Noij THM (2002) J Chrom A 970:167–181

    Article  CAS  Google Scholar 

  112. Brack W, Altenburger R, Küster E, Meissner B, Wenzel K-D, Schüürmann G (2003) Environ Toxicol Chem 22 (in press)

  113. Vasseur P (1995) 179–193

  114. Grimme LH, Faust M, Boedeker W, Altenburger R (1996) Human Ecol Risk Assess 2:426–433

    CAS  Google Scholar 

  115. White PA (2002) Mut Res 515:85–98

    Article  CAS  Google Scholar 

  116. Somani SM, Schaeffer DJ, Mack JO (1981) J Toxicol Environ Health 7:643–653

    CAS  PubMed  Google Scholar 

  117. Hermann M (1981) Mut Res 90:399–409

    Article  CAS  Google Scholar 

  118. Haugen DA, Peak MJ (1983) Mut Res 116:257–269

    Article  CAS  Google Scholar 

  119. Hayatsu H, Arimoto S, Togawa K, Makita M (1981) Mut Res 81:287–293

    Article  CAS  Google Scholar 

  120. Safe SH (1986) Annu Rev Pharmacol Toxicol 26:371–399

    Article  CAS  PubMed  Google Scholar 

  121. Burnison BK, Hodson PV, Nuttley DJ, Efler S (1996) Environ Toxicol Chem 15:1524–1531

    CAS  Google Scholar 

  122. Martel PH, Kovacs TG, O'Connor BI, Voss RH (1997) Environ Toxicol Chem 16:2375–2383

    CAS  Google Scholar 

  123. Hewitt LM, Tremblay L, Van der Kraak GJ, Solomon KR, Servos MR (1998) Environ Toxicol Chem 17:425–432

    CAS  Google Scholar 

  124. Brack W, Segner H, Möder M, Schüürmann G (2000) Environ Toxicol Chem 19:2493–2501

    CAS  Google Scholar 

  125. Hahn ME, Woodward BL, Stegemann JJ, Kennedy SW (1996) Environ Toxicol Chem 15:582–591

    CAS  Google Scholar 

  126. Routledge EJ, Sumpter JP (1996) Environ Toxicol Chem 15:241–248

    CAS  Google Scholar 

  127. Desbrow C, Routledge EJ, Brighty GC, Sumpter JP, Waldock M (1998) Environ Sci Technol 32:1549–1558

    CAS  Google Scholar 

  128. Ankley GT, Mekenyan OG, Kosian PA, Makynen EA, Mount DR, Monson PD, Call DJ (1996) SAR QSAR Environ Res 5:177–183

    CAS  Google Scholar 

  129. Kosian PA, Makynen EA, Monson PD, Mount DR, Spacie A, Mekenyan OG, Ankley GT (1998) Environ Toxicol Chem 17:1021–1033

    CAS  Google Scholar 

  130. Burkhard LP, Sheedy BR (1995) Environ Toxicol Chem 14:697–711

    CAS  Google Scholar 

  131. Shiozawa T, Tada A, Nukaya H, Watanabe T, Takahashi Y, Asanoma M, Ohe T, Sawanishi H, Katsuhara T, Sugimura T, Wakabayashi K, Terao Y (2000) Chem Res Toxicol 13:535–540

    Article  CAS  PubMed  Google Scholar 

  132. Kami H, Watanabe T, Takemura S, Kameda Y, Hirayama T (2000) Chem Res Toxicol 13:165–169

    Article  CAS  PubMed  Google Scholar 

  133. Castillo M, Alonso MC, Riu J, Barcelo D (1999) Environ Sci Technol 33:1300–1306

    CAS  Google Scholar 

  134. Ouellet M, Bonin J, Rodrigue J, DesGranges J-L, Lair S (1997) J Wildlife Disease 33:95–104

    CAS  Google Scholar 

  135. Lewtas J (1988) Fund Appl Toxicol 10:571–589

    CAS  Google Scholar 

  136. Rosenkranz HS, McCoy EC, Sanders DR, Butler M, Kiriazides DK, Mermelstein R (1980) Science 209:1039–1042

    CAS  PubMed  Google Scholar 

  137. Hayes MA, Smith IR, Rushmore TH, Crane TL, Thorn C, Kocal TE, Ferguson HW (1990) Sci Tot Environ 94:105–123

    CAS  Google Scholar 

  138. Harshbarger JC, Clark JB (1990) Sci Tot Environ 94:1–32

    CAS  Google Scholar 

  139. Myers MS, Landahl JT, Krahn MM, McCain BB (1991) Environ Health Persp 90:7–15

    CAS  Google Scholar 

  140. Vahl HH, Karbe L, Prieto-Alamo MJ, Pueyo C (1995) J Aquat Ecosyst Health 4:277–283

    Google Scholar 

  141. Kronberg L, Holmborn B, Reunanen M, Tikkanen L (1988) Environ Sci Technol 22:1097–1103

    CAS  Google Scholar 

  142. Purdom CE, Hardiman PA, Bye VJ, Eno NC, Tyler CR, Sumpter JP (1994) Chem Ecology 8:275–285

    CAS  Google Scholar 

  143. Routledge EJ, Sheahan D, Desbrow C, Brighty GC, Waldock M, Sumpter JP (1998) Environ Sci Technol 32:1559–1565

    Article  CAS  Google Scholar 

  144. Arey J, Harger WP, Helmig D, Atkinson R (1992) Mut Res 281:67–76

    Article  CAS  Google Scholar 

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  1. Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research, Permoserstraße 15, , 04318, Leipzig, Germany

    Werner Brack

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Brack, W. Effect-directed analysis: a promising tool for the identification of organic toxicants in complex mixtures?. Anal Bioanal Chem 377, 397–407 (2003). https://doi.org/10.1007/s00216-003-2139-z

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