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Simultaneous assay of glucose, lactate, L-glutamate and hypoxanthine levels in a rat striatum using enzyme electrodes based on neutral red-doped silica nanoparticles

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Abstract

An electrochemical method suitable for the simultaneous measurement of cerebral glucose, lactate, L-glutamate and hypoxanthine concentrations from in vivo microdialysis sampling has been successfully performed for the first time using a neutral red-doped silica (NRDS) nanoparticle-derived enzyme sensor system. These uniform NRDS nanoparticles (about 50±3 nm) were prepared by a water-in-oil (W/O) microemulsion method, and characterized by a TEM technique. The neutral red-doped interior maintained its high electron-activity, while the exterior nano-silica surface prevented the mediator from leaching out into the aqueous solution, and showed high biocompability. These nanoparticles were then mixing with the glucose oxidase (GOD), lactate oxidase (LOD), L-glutamate oxidase (L-GLOD) or xanthine oxidase (XOD), and immobilized on four glassy carbon electrodes, respectively. A thin Nafion film was coated on the enzyme layer to prevent interference from molecules such as ascorbic acid and uric acid in the dialysate. The high sensitivity of the NRDS modified enzyme electrode system enables the simultaneous monitoring of trace levels of glucose, L-glutamate, lactate and hypoxanthine in diluted dialysate samples from a rat striatum.

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References

  1. Boutelle MG, Fellows LK, Cook C (1992) Anal Chem 64:1790–1794

    CAS  PubMed  Google Scholar 

  2. Shram NF, Netchiporouk LI, Martelet C, Jaffrezic-Renault N, Bonnet C, Cespuglio R (1998) Anal Chem 70:2618–2622

    Article  CAS  PubMed  Google Scholar 

  3. Hashimoto A, Kanda J, Oka T (2000) Brain Res Bull 53:347–351

    Article  CAS  PubMed  Google Scholar 

  4. Mao LQ, Xu F, Xu Q, Jin LT (2001) Anal Biochem 292:94–101

    Article  CAS  PubMed  Google Scholar 

  5. Mao LQ, Yamamoto K (2000) Anal Chim Acta 415:143–150

    Article  CAS  Google Scholar 

  6. Llaudet E, Botting NP, Crayston JA, Dale N (2003) Biosens Bioelectron 18:43–52

    Article  CAS  PubMed  Google Scholar 

  7. Netchiporouk LI, Shram NF, Renault NJ, Martelet C, Cespuglio R (1996) Anal Chem 68:4358–4364

    Article  CAS  PubMed  Google Scholar 

  8. Ellmerer M, Schaupp L, Trajanoski Z, Jobst G, Moser I, Urban G, Skrabal F, Wash P (1998) Biosens Bioelectron 13:1007–1013

    Article  CAS  PubMed  Google Scholar 

  9. Petrou PS, Moser I, Jobst G (2003) Biosens Bioelectron 18:613–619

    Article  CAS  PubMed  Google Scholar 

  10. Woitzik J, Abromeit N, Schaefer F (2001) Anal Biochem 289:10–17

    Article  CAS  PubMed  Google Scholar 

  11. Zhou JX, Heckert DM, Zuo H, Lunte CE, Lunte SM (1999) Anal Chim Acta 379:307–317

    Article  CAS  Google Scholar 

  12. Malone MA, Zuo H, Lunte SM, Smyth MR (1995) J Chromatogr A 700:73–80

    Article  CAS  Google Scholar 

  13. Xu F, Wang L, Gao MN, Jin LT, Jin JY (2002) Talanta 57:365–373

    CAS  Google Scholar 

  14. Obrenovitch TP, Zilkha E (2001) Methods 23:63–71

    Article  CAS  PubMed  Google Scholar 

  15. Zhu NN, Cai H, He PG, Fang YZ (2003) Anal Chim Acta 481:181–189

    Article  CAS  Google Scholar 

  16. Strehlitz B, Grundig B, Schumacher W, Kroneck PMH, Vorlop KD, Kotte H (1996) Anal Chem 68:807–816

    Article  CAS  Google Scholar 

  17. Abo M, Ogasawara Y, Tanaka Y, Okubo A, Yamazaki S (2003) Biosens Bioelectron 18:735–739

    Article  CAS  PubMed  Google Scholar 

  18. Zhu M, Han SB, Yuan ZB (2000) J Electroanal Chem 480:255–261

    Article  CAS  Google Scholar 

  19. Harrison DJ, Turner RFB, Baltcrs HP (1988) Anal Chem 60:2002–2007

    CAS  PubMed  Google Scholar 

  20. Yu JH, Liu SQ, Ju HX (2003) Biosens Bioelectron 19:401–409

    Article  CAS  PubMed  Google Scholar 

  21. Patel NG, Erlenkotter A, Cammann K, Chemnitius GC (2000) Sens Actuators B-Chem 67:134–141

    Google Scholar 

  22. Prada AGV, Peña N, Parrado C, Reviejo AJ, Pingarrón JM (2004) Talanta 62:896–903

    Article  Google Scholar 

  23. Arriagada FJ, Osseo-Asare K (1999) J Colloid Interf Sci 211:210–220

    Article  CAS  Google Scholar 

  24. Santra SS, Zhang P, Wang KM, Tapec Rovelyn, Tan WH (2001) Anal Chem 73:4988–4993

    Article  CAS  PubMed  Google Scholar 

  25. Bao XM, Su Y (1991) The stereotaxic atlas of the rat brain. People’s Healthy Press, Beijing

    Google Scholar 

  26. Sun YX, Ye BX, Zhang WM, Zhou XY (1998) Anal Chim Acta 363:75–80

    Article  CAS  Google Scholar 

  27. Brown RS, Luong JHT (1995) Anal Chim Acta 310:419–427

    Article  CAS  Google Scholar 

  28. Yao T, Yano T, Nishino H (2004) Anal Chim Acta 510:53–59

    Article  CAS  Google Scholar 

  29. Yao T, Yano T (2004) Talanta 63:771–775

    Article  CAS  Google Scholar 

  30. Stover JF, Lowitzsch K, Kempski OS (1997) Neurosci Lett 238:25–28

    Article  CAS  PubMed  Google Scholar 

  31. Lada MW, Vickroy TW, Kennedy RT (1997) Anal Chem 69:4560–4565

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Financial support is acknowledged from the National Natural Science Foundation of China (No. 20175006, 20305007) and the specialized Research Fund for Nano Technology from Shanghai (No. 0214nm078 and No. 0359nm002).

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China

    Fen-Fen Zhang, Qiao Wan, Chen-Xin Li, Xiao-Li Wang, Yue-Zhong Xian & Li-Tong Jin

  2. Department of Electronic Engineering, East China Normal University, Shanghai 200062, China

    Zi-Qiang Zhu

  3. BAS Co. Ltd., No.36-4, 1-Chome, Oshiage, Sumida-Ku, Tokyo 131, Japan

    Katsunobu Yamamoto

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  1. Fen-Fen Zhang
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  2. Qiao Wan
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  3. Chen-Xin Li
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  6. Yue-Zhong Xian
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Correspondence to Li-Tong Jin.

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Zhang, FF., Wan, Q., Li, CX. et al. Simultaneous assay of glucose, lactate, L-glutamate and hypoxanthine levels in a rat striatum using enzyme electrodes based on neutral red-doped silica nanoparticles. Anal Bioanal Chem 380, 637–642 (2004). https://doi.org/10.1007/s00216-004-2804-x

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  • DOI: https://doi.org/10.1007/s00216-004-2804-x

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