Abstract
The fluorescence of fluoresceinisothiocyanate-labeled concanavalin A (FITC-Con A) was quenched by forming an FITC-Con A–glycogen conjugate and dequenched upon addition of sugars to the conjugate solution due to disaggregation of the conjugate. However, fluorescence quenching was barely observed upon formation of FITC-Con A–dextran conjugate. The sugar-induced fluorescence response of the FITC-Con A–glycogen conjugate depended significantly on the type of sugar: methylated α-D-glucose and α-D-mannose both induced high and rapid responses, while the responses to D-mannose and D-glucose were moderate. In contrast, no response was observed in the presence of D-galactose due to a lack of affinity to Con A. Thus, it is apparent that D-glucose and other sugars can be detected via the fluorescence of the FITC-Con A–glycogen conjugate.
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Acknowledgement
This work was supported in part by a Grant-in-Aid (No. 16390013) from Japan Society for Promotion of Sciences (JSPS).
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Sato, K., Anzai, Ji. Fluorometric determination of sugars using fluorescein-labeled concanavalin A–glycogen conjugates. Anal Bioanal Chem 384, 1297–1301 (2006). https://doi.org/10.1007/s00216-005-0279-z
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DOI: https://doi.org/10.1007/s00216-005-0279-z