Abstract
We have developed an ammonia-sensitive material by coupling the Berthelot reaction to our polymerized crystalline colloidal array (PCCA) technology. The material consists of a periodic array of highly charged colloidal particles (110 nm diameter) embedded in a poly(hydroxyethyl acrylate) hydrogel. The particles have a lattice spacing such that they Bragg-diffract visible light. In the Berthelot reaction, ammonia, hypochlorite, and phenol react to produce the dye molecule indophenol blue in an aqueous solution. We use this reaction in our sensor by covalently attaching 3-aminophenol to the hydrogel backbone, which forms cross-links through the Berthelot mechanism. Ammonia reacts with hypochlorite, forming monochloramine, which then reacts with a pendant aminophenol to form a benzoquinone chlorimine. The benzoquinone chlorimine reacts with another pendant aminophenol to form a cross-link. The creation of new cross-links causes the hydrogel to shrink, which reduces the lattice spacing of the embedded colloidal array. This volume change results in a blue-shift in the diffracted light proportional to the concentration of NH3 in the sample. We demonstrate that the NH3 photonic crystal sensing material is capable of quantitative determination of concentrations in the physiological range (50–350 μmol NH3 L−1) in human blood serum.
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Huizenga JR, Gips CH, Tangerman A (1996) Ann Clin Biochem 33:23
Tietz NW (1995) Clinical laboratory guide to laboratory tests, 3rd edn. WB Saunders, Philadelphia
Pesce AJ, Kaplan LA (1987) Methods in clinical chemistry. CV Mosby Company, St. Louis
Hazell AS, Butterworth RF (1999) Exp Biol and Med 222:99
Campion M (2003) Blood ammonia: a critical measurement. BIMDG Bulletin Spring 13
Bachmann C (2003) Eur J of Pediatr 162:S29
Mondzac A, Ehrlich GE, Seegmiller JE (1965) J Lab Clin Med 66:526
Van Anken HC, Schiphorst ME (1974) A Clin Chim Acta 56:151
Vitros chemistry products AMON slides (2005) www.orthoclinical.com
Huizenga JR, Tangerman A, Gips CH (1994) Ann Clin Biochem 31:529
Mann LT (1963) Anal Chem 35:2179
Reay PF (1985) Anal Chim Acta 176:275
Lau KT, Edwards S, Diamond D (2004) Sens Actuators B 98:12
Daridon et al (2001) Sens Actuators B 76:235
Zellmer S, Katenborn G, Rothe U, Lehnich H, Lasch J, Pauer HD (1999) Anal Biochem 273:163
Berthelot M (1859) Rep Chim Appl I 284
Searle PL (1984) Analyst 109:549
Gips CH, Reitsema A (1971) Clin Chem Acta 33:257
Ngo TT, Phan APH, Yam CF, Lenhoff HM (1982) Anal Chem 54:46
Krieger IM, O’Neill FM (1968) J Am Chem Soc 90:3114
Hiltner PA, Krieger IM (1969) J Phys Chem 73:2386
Hiltner PA, Papir YS, Krieger IM (1971) J Phys Chem 75:1881
Carlson RJ, Asher SA (1984) Appl Spec 38:297
Runquist PA, Photinos P, Jagannathan S, Asher SA (1989) J Chem Phys 91:4932
Asher SA, Holtz JH, Liu L, Wu Z (1994) J Am Chem Soc 116:4997
Weissman JM, Sunkara HB, Tse AS, Asher SA (1996) Science 274:959
Ito K, Nakamura H, Ise N (1986) J Chem Phys 85:6136
Monovoukas Y, Gast AP (1989) J Colloid Interface Sci 128:533
Okubo T (1988) Acc Chem Res 21:281
Asher SA, U.S. Patents 4,627,689 (1986), 4,632,517 (1986), 5,281,370 (1994), 5,452,123 (1995)
Holtz JH, Asher SA (1997) Nature 389:829
Holtz JH, Holtz JS, Munro C, Asher SA (1998) Anal Chem 70:780
Pan G, Kesavamoorthy R, Asher SA (1998) J Am Chem Soc 120:6525
Goponenko AV, Asher SA J Am Chem Soc 127:10753
Harfmann RG, Crouch SR (1989) Talanta 36:261
Davies MJ (2005) Biochim Biophys Acta 1703:93
Hawkins CL, Pattison DI, Davies MJ (2003) Amino Acids 25:259
Acknowledgements
We gratefully acknowledge NIH grants# 1 R01 GM 58821-01 and 2R01 DK 55348-03A1.
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Kimble, K.W., Walker, J.P., Finegold, D.N. et al. Progress toward the development of a point-of-care photonic crystal ammonia sensor. Anal Bioanal Chem 385, 678–685 (2006). https://doi.org/10.1007/s00216-006-0453-y
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DOI: https://doi.org/10.1007/s00216-006-0453-y