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Capacitive biosensor for detection of endotoxin

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Abstract

A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 × 10−13 M and linearity ranging from 1.0 × 10−13 to 1.0 × 10−10 M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P < 0.05).

The flow-injection capacitive biosensor system and the capacitive properties of the transducer surface, where CSAM is the capacitance change of the self-assembled thiol monolayer, CP is the capacitance change of the protein layer, Ca is the capacitance change of the analyte layer and CTotal is the total capacitance change measured at the working electrode/solution interface (modified from Limbut et al., 2006. Biosens Bioelectron 22: 233-240)

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Acknowledgements

This project was supported The Royal Golden Jubilee PhD Program supported by Thailand Research Fund; The Swedish Research Council/Sida-Research Link Projects, The Swedish Center for Bioseparation; Center for Innovation in Chemistry: Postgraduate Education and Research Program in Chemistry (PERCH-CIC), Thailand; and Graduate School, Prince of Songkla University, Hat Yai, Thailand.

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Authors and Affiliations

  1. Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Box 124, 221 00, Lund, Sweden

    Warakorn Limbut, Martin Hedström & Bo Mattiasson

  2. Biophysics Research Unit: Biosensors and Biocurrents, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Warakorn Limbut, Panote Thavarungkul & Proespichaya Kanatharana

  3. Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Warakorn Limbut & Proespichaya Kanatharana

  4. Department of Physics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Panote Thavarungkul

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  1. Warakorn Limbut
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  2. Martin Hedström
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  3. Panote Thavarungkul
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  4. Proespichaya Kanatharana
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  5. Bo Mattiasson
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Correspondence to Bo Mattiasson.

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Limbut, W., Hedström, M., Thavarungkul, P. et al. Capacitive biosensor for detection of endotoxin. Anal Bioanal Chem 389, 517–525 (2007). https://doi.org/10.1007/s00216-007-1443-4

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  • DOI: https://doi.org/10.1007/s00216-007-1443-4

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