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Room temperature ionic liquid doped DNA network immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide

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Abstract

A novel electrochemical H2O2 biosensor was constructed by embedding horseradish peroxide (HRP) in a 1-butyl-3-methylimidazolium tetrafluoroborate doped DNA network casting on a gold electrode. The HRP entrapped in the composite system displayed good electrocatalytic response to the reduction of H2O2. The composite system could provide both a biocompatible microenvironment for enzymes to keep their good bioactivity and an effective pathway of electron transfer between the redox center of enzymes, H2O2 and the electrode surface. Voltammetric and time-based amperometric techniques were applied to characterize the properties of the biosensor. The effects of pH and potential on the amperometric response to H2O2 were studied. The biosensor can achieve 95% of the steady-state current within 2 s response to H2O2. The detection limit of the biosensor was 3.5 μM, and linear range was from 0.01 to 7.4 mM. Moreover, the biosensor exhibited good sensitivity and stability. The film can also be readily used as an immobilization matrix to entrap other enzymes to prepare other similar biosensors.

Horseradish peroxidase (HRP) embedded in a 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM·BF 4 ) doped DNA network can be used to fabricate a HRP sensor for the determination of H2O2

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Acknowledgement

This work was supported by the National Natural Science Foundation of China.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Graduate School of the Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China

    Cunlan Guo, Yonghai Song, Hui Wei, Peicai Li, Li Wang, Lanlan Sun, Yujing Sun & Zhuang Li

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  1. Cunlan Guo
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  2. Yonghai Song
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  3. Hui Wei
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  4. Peicai Li
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  5. Li Wang
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  6. Lanlan Sun
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  7. Yujing Sun
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  8. Zhuang Li
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Correspondence to Zhuang Li.

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Guo, C., Song, Y., Wei, H. et al. Room temperature ionic liquid doped DNA network immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide. Anal Bioanal Chem 389, 527–532 (2007). https://doi.org/10.1007/s00216-007-1478-6

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  • DOI: https://doi.org/10.1007/s00216-007-1478-6

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