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Electro-chemiluminescent biosensing

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Abstract

The present review draws a general picture of the bioanalytical applications of electro-chemiluminescent reactions (ECL). Only the two main ECL reactions—i.e. the luminol-based and \( {\text{Ru}}{\left( {{\text{bpy}}} \right)}^{{2 + }}_{3} \)-based reactions—are considered for application in the fields of enzyme biosensors, immunochemical biosensors, DNA biosensors, and biochips. The mechanism, principle, and experimental conditions of these two reactions are described. Then, for each category of analytical tools, experimental set-ups and performances are presented and discussed.

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References

  1. Isacsson U, Wettermark G (1974) Anal Chim Acta 68:339–362

    Article  CAS  Google Scholar 

  2. Stedman DH, Fraser ME (1985) Analytical applications of gas phase chemiluminescence. In: JG Burr (ed) Chemi- and Bioluminescence. Dekker, New York pp 439–468

    Google Scholar 

  3. Blum LJ (1997) Bio-, chemi-luminescent sensors. World Scientific, Singapore

    Google Scholar 

  4. Jirka GP, Martin AF, Nieman TA (1993) Anal Chim Acta 284:345–349

    Article  CAS  Google Scholar 

  5. Roswell DF, White EH (1978) The chemiluminescence of luminol and related hydrazides. In: Fleischer S, Fleischer B (eds) Methods in enzymology. Academic Press, London, pp 409–423

    Google Scholar 

  6. Sakura S (1992) Anal Chim Acta 262:49–57

    Article  CAS  Google Scholar 

  7. Lee W-Y (1997) Microchim Acta 127:19–39

    CAS  Google Scholar 

  8. Knight AW, Greenway GM (1994) Analyst 119:879–890

    Article  CAS  Google Scholar 

  9. Beideman FE, Hercules DM (1979) J Phys Chem 83:2203–2209

    Article  CAS  Google Scholar 

  10. Kakhr A, Mugnier Y, Laviron E (1983) Electrochim Acta 28:1897–1898

    Article  Google Scholar 

  11. Bard AJ (2004) Electrogenerated chemiluminescence. Marcel Dekker, New York

    Google Scholar 

  12. Tokel NE, Bard AJ (1972) J Am Chem Soc 94:2862–2863

    Article  CAS  Google Scholar 

  13. Wilson R (2001) Electroanalysis 13:1083–1092

    Article  CAS  Google Scholar 

  14. Leland JK, Powell MJ (1990) J Electrochem Soc 137:3127–3131

    Article  CAS  Google Scholar 

  15. Marquette CA, Blum LJ (1999) Anal Chim Acta 381:1–10

    Article  CAS  Google Scholar 

  16. Marquette CA, Blum LJ (1998) Sens Actuators B 51:100–106

    Article  Google Scholar 

  17. Laespada MF, Pavon JP, Cordero BM (1996) Anal Chim Acta 327:253–260

    Article  CAS  Google Scholar 

  18. Marquette CA, Ravaud S, Blum LJ (2000) Anal Lett 33:1779–1796

    Article  CAS  Google Scholar 

  19. Tsafack VC, Marquette CA, Leca B, Blum LJ, Tsafack VC (2000) Analyst 125:151–155

    Article  CAS  Google Scholar 

  20. Marquette CA, Leca BD, Blum LJ (2001) Luminescence 16:159–165

    Article  CAS  Google Scholar 

  21. Blum LJ (1993) Enzyme Microb Technol 15:407–411

    Article  CAS  Google Scholar 

  22. Martin AF, Nieman TA (1993) Anal Chim Acta 281:475–481

    Article  CAS  Google Scholar 

  23. Jameison F, Sanchez RI, Dong L, Leland JK, Yost D, Martin MT (1996) Anal Chem 68:1298–1302

    Article  CAS  Google Scholar 

  24. Yokoyama K, Sasaki S, Ikebukuro K, Takeuchi T, Karube I, Tokitsu Y, Masuda Y (1994) Talanta 41:1035–1040

    Article  CAS  Google Scholar 

  25. Rubinstein I, Martin CR, Bard AJ (1983) Anal Chem 55:1580–1582

    Article  CAS  Google Scholar 

  26. Hersh LS, Vann WP, Wilheim SA (1979) Anal Biochem 93:267–271

    Article  CAS  Google Scholar 

  27. Calvo-Munoz ML, Dupont-Filliard A, Billon M, Guillerez S, Bidan G, Marquette C, Blum L (2005) Bioelectrochemistry 66:139–143

    Article  CAS  Google Scholar 

  28. Wilson R, Barker MH, Schiffrin DJ, Abuknesha R (1997) Biosens Bioelectron 12:277–286

    Article  CAS  Google Scholar 

  29. Wilson R, Clavering C, Hutchinson A (2003) Analyst 128:480–485

    Article  CAS  Google Scholar 

  30. Wilson R, Clavering C, Hutchinson A (2003) Anal Chem 75:4244–4249

    Article  CAS  Google Scholar 

  31. Blackburn GF, Shah HP, Kenten JH, Leland J, Kamin RA, Link J, Peterman J, Powell MJ, Shah A, Talley DB (1991) Clin Chem 37:1534–1539

    CAS  Google Scholar 

  32. Kenten JH, Casadei J, Link J, Lupold S, Willey J, Powell M, Rees A, Massey R (1991) Clin Chem Lab Med 37:1626–1632

    CAS  Google Scholar 

  33. Knight AW, Greenway GM (1996) Analyst 121:101R–106R

    Article  CAS  Google Scholar 

  34. Terpetschnig E, Szmacinski H, Lakowicz JR (1995) Anal Biochem 227:140–147

    Article  CAS  Google Scholar 

  35. Terpetschnig E, Szmacinski H, Malak H, Lakowicz JR (1995) Biophys J 68:342–350

    Article  CAS  Google Scholar 

  36. Kibbey MC, MacAllan D, Karaszkiewicz JW (2000) J Assoc Lab Automat 5:45–48

    Article  CAS  Google Scholar 

  37. Deaver DR (1995) Nature 377:758–760

    Article  CAS  Google Scholar 

  38. Gatto-Menking DL, Yu H, Bruno JG, Goode MT, Miller M, Zulich AW (1995) Biosens Bioelectron 10:501–507

    Article  CAS  Google Scholar 

  39. Fähnrich K, O’Sullivan CK, Guilbault GG (2000) Disposable biosensor based on electrogenerated chemiluminescence (ECL). in Europt(r)ode V, Lyon, France

  40. Michel PE, de Rooij NF, Koudelka-Hep M, Fahnrich KA, Sullivan CK, Guilbault GG (1999) J Electroanal Chem 474:192–194

    Article  CAS  Google Scholar 

  41. Yang M, Liu C, Qian K, He P, Fang Y (2002) Analyst 127:1267–1271

    Article  CAS  Google Scholar 

  42. Fernando Patolsky EKIW (2002) Angew Chem Int Ed 41:3398–3402

    Article  Google Scholar 

  43. Arcamone F (1983) Doxorubicin Anticancer antibiotics. Academic Press, New York

    Google Scholar 

  44. Siddiqi AM, Jennings VM, Kidd MR, Actor JK, Hunter RL (1996) J Clin Lab Anal 10:423–431

    Article  CAS  Google Scholar 

  45. Motmans K, Raus J, Vandevyver C (1996) J Immunol Methods 190:107–116

    Article  CAS  Google Scholar 

  46. O’Connell CD, Juhasz A, Kuo C, Reeder DJ, Hoon DSB (1998) Clin Chem 44:1161–1669

    CAS  Google Scholar 

  47. Gudibande SR, Kenten JH, Link J, Friedman K, Massey RJ (1992) Mol Cell Probes 6:495–503

    Article  CAS  Google Scholar 

  48. DiCesare J, Grossman B, Katz E, Picozza E, Ragusa R, Woudenberg T (1993) BioTechniques 15:152–157

    CAS  Google Scholar 

  49. Xu X, Bard AJ (1995) J Am Chem Soc 117:2627–2631

    Article  CAS  Google Scholar 

  50. Xu X, Yang HC, Mallouk TE, Bard AJ (1994) J Am Chem Soc 116:8386–8387

    Article  CAS  Google Scholar 

  51. Marquette CA, Blum LJ (2003) Sens Actuators B 90:112–117

    Article  CAS  Google Scholar 

  52. Marquette CA, Degiuli A, Blum LJ (2003) Biosens Bioelectron 19:433–439

    Article  CAS  Google Scholar 

  53. Marquette CA, Thomas D, Degiuli A, Blum LJ (2003) Anal Bioanal Chem 377:922–928

    Article  CAS  Google Scholar 

  54. Corgier BP, Marquette CA, Blum LJ (2005) Anal Chim Acta 538:1–7

    Article  CAS  Google Scholar 

  55. Fiaccabrino GC, de Rooij NF, Koudelka-Hep M (1998) Anal Chim Acta 359:263–267

    Article  CAS  Google Scholar 

  56. Michel PE, van der Wal PD, Fiaccabrino GC, de Rooij NF, Koudelka-Hep M (1999) Electroanalysis 11:1361–1367

    Article  CAS  Google Scholar 

  57. Michel PE, Fiaccabrino GC, de Rooij NF, Koudelka-Hep M (1999) Anal Chim Acta 392:95–103

    Article  CAS  Google Scholar 

  58. L’Hostis E, Michel PE, Fiaccabrino GC, Strike DJ, de Rooij NF, Koudelka-Hep M (2000) Sens Actuators B 64:156–162

    Article  Google Scholar 

  59. Arora A, de Mello AJ, Manz A (1997) Anal Commun 34:393–395

    Article  CAS  Google Scholar 

  60. Marquette CA, Blum LJ (2004) Biosens Bioelectron 20:197–203

    Article  CAS  Google Scholar 

  61. Glezer EN, Jonhson K, Debad JD, Tsionsky M, Jeffrey-Coker B, Clinton C, Kinshbaugh A, Leland JK, Billadeau M, Leytner S, Altunata S, Sigal GB, Wilbur JL, Biebuyck HA, Wholstader JN (2002) Electrochemiluminescent microarrays: a new tool for drug discovery and life science research, 224th ACS National Meeting, Boston, USA

  62. Patolsky F, Weizmann Y, Willner I (2002) J Am Chem Soc 124:770–772

    Article  CAS  Google Scholar 

  63. Trau D, Lee TMH, Lao AIK, Lenigk R, Hsing IM, Ip NY, Carles MC, Sucher NJ (2002) Anal Chem 74:3168–3173

    Article  CAS  Google Scholar 

  64. Wegner GJ, Lee HJ, Corn RM (2002) Anal Chem 74:5161–5168

    Article  CAS  Google Scholar 

  65. Long YT, Li CZ, Sutherland TC, Kraatz HB, Lee JS (2004) Anal Chem 76:4059–4065

    Article  CAS  Google Scholar 

  66. Coche-Guerente L, Cosnier S, Innocent C, Mailley P, Moutet JC, Morelis RM, Leca B, Coulet PR (1993) Electroanalysis 5:647–652

    Article  CAS  Google Scholar 

  67. Liljegren G, Nyholm L (2003) Analyst 128:232–236

    Article  CAS  Google Scholar 

  68. Marquette CA, Laurenceau E, Mallet F, Chaix C, Blum LJ, Mandrand B (2005) Anal Biochem 340:14–23

    Article  CAS  Google Scholar 

  69. Guadarrama A, Rodríguez-Mendez ML, Sanz C, Ríos JL, De Saja JA (2001) Anal Chim Acta 432:283–292

    Article  CAS  Google Scholar 

  70. Cao T, Chen J, Cao W, Wei F, Liao W, Zhao X, Jiao X (2003) Langmuir 19:8127–8129

    Article  CAS  Google Scholar 

  71. Corgier BP, Laurent A, Perriat P, Blum LJ, Marquette CA (2007) Angew Chem Int Ed 46:4108–4110

    Article  CAS  Google Scholar 

  72. Corgier BP, Marquette CA, Blum LJ (2005) J Am Chem Soc 127:18328–18332

    Article  CAS  Google Scholar 

  73. Corgier BP, Marquette CA, Blum LJ (2006) Biosens Bioelectron 22:1522–1526

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Génie Enzymatique et Biomoléculaire, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1 - CNRS 5246 ICBMS, Bâtiment CPE - 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France

    Christophe A. Marquette & Loïc J. Blum

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  1. Christophe A. Marquette
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  2. Loïc J. Blum
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Correspondence to Christophe A. Marquette.

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Marquette, C.A., Blum, L.J. Electro-chemiluminescent biosensing. Anal Bioanal Chem 390, 155–168 (2008). https://doi.org/10.1007/s00216-007-1631-2

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