Skip to main content
SpringerLink
Log in

A capacitive biosensor for detection of staphylococcal enterotoxin B

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A sensitive method for the detection of staphylococcal enterotoxin B (SEB) using a flow-injection capacitive biosensor is presented. SEB was purified from a crude culture filtrate of Staphylococcus aureus through three chromatographic steps. The first two steps were based on ion-exchange chromatography, and the last step was carried out on a gel filtration column. The SEB recovery values after the purification stages were 88%, 74%, and 12%, respectively. A horseradish peroxidase labeled antistaphylococcal enterotoxin B was prepared by the periodate method and was further employed in a sandwich-enzyme-linked immunosorbent assay (ELISA) for the determination of SEB concentrations in different samples obtained during the processing of the crude filtrate. The capacitive biosensor could detect SEB concentrations as low as 0.3 pg ml−1 with a linearity ranging from 2.8 pg ml−1 to 2.8 ng ml−1 under optimized conditions. The response time was about 10 min. A good agreement was achieved between the developed capacitive biosensor system and ELISA as a reference method for detection of SEB levels in different purification samples. The newly developed sensor has the benefits of simplicity, high sensitivity, and multiple use capability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Fueyo JM, Martin MC, Gonzalez-Hevia MA, Mendoza MC (2001) Int J Food Microbiol 67:139–145

    Article  CAS  Google Scholar 

  2. McCormick JK, Yarwood JM, Schlievert PM (2001) Annu Rev Microbiol 55:77–104

    Article  CAS  Google Scholar 

  3. Dinges MM, Orwin PM, Schlievert PM (2000) Clin Microbiol Rev 13:16–34

    Article  CAS  Google Scholar 

  4. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, Nesme X, Etienne J, Vandenesch F (2002) Infect Immun 70:631–641

    Article  CAS  Google Scholar 

  5. Omoe K, Ishikawa M, Shimoda Y, Hu DL, Ueda S, Shinagawa K (2002) J Clin Microbiol 40:857–862

    Article  CAS  Google Scholar 

  6. Jarraud S, Peyrat MA, Lim A, Tristan A, Bes M, Mougel C, Etienne J, Vandenesch F, Bonneville M, Lina G (2001) J Immunol 166:669–677

    CAS  Google Scholar 

  7. Llewelyn M, Cohen J (2002) Lancet Infect Dis 2:156–162

    Article  CAS  Google Scholar 

  8. Harris TO, Grossman D, Kappler JW, Marrack P, Rich RR, Betley MJ (1993) Infect Immun 61:3175–3183

    CAS  Google Scholar 

  9. Burnett JC, Henchal EA, Schmaljohn A, Bavari S (2005) Nat Rev Drug Discov 4:281–297

    Article  CAS  Google Scholar 

  10. Poli MA, Rivera VR, Neal D (2002) Toxicon 40:1723–1726

    Article  CAS  Google Scholar 

  11. Balaban N, Rasooly A (2000) Int J Food Microbiol 61:1–10

    Article  CAS  Google Scholar 

  12. Walt DR, Franz DR (2000) Anal Chem 72:738A–746A

    Article  CAS  Google Scholar 

  13. Gill DM (1982) Microbiol Rev 46:86–94

    CAS  Google Scholar 

  14. Lin H, Tsai W (2003) Biosens Bioelectron 18:1479–1483

    Article  CAS  Google Scholar 

  15. Lam MT, Boulet CA, Chris Le X (2002) Anal Chim Acta 457:21–28

    Article  CAS  Google Scholar 

  16. Rasooly A (2001) J Food Prot 64:37–43

    CAS  Google Scholar 

  17. King KD, Andersson GP, Bullock KE, Regina MJ, Saaski EW, Ligler FS (1999) Biosens Bioelectron 14:163–170

    Article  CAS  Google Scholar 

  18. Callahan JH, Shefcheck KJ, Williams TL, Musser SM (2006) Anal Chem 78:1789–1800

    Article  CAS  Google Scholar 

  19. Taitt CR, Golden JP, Feldstein MJ, Cras JJ, Hoffman KE, Ligler FS (2000) Biosens Bioelectron 14:785–794

    Article  Google Scholar 

  20. Nedelkov D, Nelson RW (2003) Appl Environ Microbiol 69:5215–5215

    Article  CAS  Google Scholar 

  21. Nedelkov D, Rasooly A, Nelson RW (2000) Int J Food Microbiol 60:1–13

    Article  CAS  Google Scholar 

  22. O’Brien T, Johnson LH, Aldrich JL, Allen SG, Liang LT, Plummer AL, Krak SJ, Boiarski AA (2000) Biosens Bioelectron 14:815–828

    Article  CAS  Google Scholar 

  23. Di Pinto A, Forte VT, Ciccarese G, Conversano MC, Tantillo GM (2004) J Food Saf 24:231–238

    Article  Google Scholar 

  24. Ruan C, Zeng K, Varghese OK, Grimes CA (2004) Biosens Bioelectron 20:585–591

    Article  CAS  Google Scholar 

  25. Medina MB (2006) J Agr Food Chem 54:4937–4942

    Article  CAS  Google Scholar 

  26. Sapsford KE, Taitt CR, Loo N, Ligler FS (2005) Appl Environ Microbiol 71:5590–5592

    Article  CAS  Google Scholar 

  27. Ligler FS, Taitt CR, Shriver-Lake LC, Sapsford KE, Shubin Y, Golden JP (2003) Anal Bioanal Chem 377:469–477

    Article  CAS  Google Scholar 

  28. Delehanty JB, Ligler FS (2002) Anal Chem 74:5681–5687

    Article  CAS  Google Scholar 

  29. Alefantis T, Grewal P, Ashton J, Khan AS, Valdes JJ, Del Vecchio VG (2004) Mol Cell Probes 18:379–382

    Article  CAS  Google Scholar 

  30. Miyamoto T, Kamikado H, Kobayashi H, Honjoh K, Lio M (2003) J Food Prot 66:1222–1226

    CAS  Google Scholar 

  31. Peruski AH, Johnson LH, Peruski LF (2002) J Immunol Methods 263:35–41

    Article  CAS  Google Scholar 

  32. Maraldo D, Mutharasan R (2007) Anal Chem 79:7636–7643

    Article  CAS  Google Scholar 

  33. Bhatti AR, Siddiqui YM, Micusan VV (1994) J Microbiol Methods 19:179–187

    Article  CAS  Google Scholar 

  34. Limbut W, Hedström M, Thavarungkul P, Kanatharana P, Mattiasson B (2007) Anal Bioanal Chem 389:517–525

    Article  CAS  Google Scholar 

  35. Limbut W, Kanatharana P, Matiasson B, Asawatreratanakul P, Thavarungkul P (2006) Biosens Bioelectron 22:233–240

    Article  CAS  Google Scholar 

  36. Hedström M, Galaev IY, Mattiasson B (2005) Biosens Bioelectron 21:41–48

    Article  CAS  Google Scholar 

  37. Hu S-Q, Xie Z-M, Lei CX, Shen G-L, Yu R-Q (2005) Sens Acuators B 106:641–647

    Article  CAS  Google Scholar 

  38. Hu SQ, Wu ZY, Zhou YM, Cao ZX, Shen GL, Yu RQ (2002) Anal Chim Acta 458:297–304

    Article  CAS  Google Scholar 

  39. Berggren C, Bjarnason B, Johansson G (2001) Electroanalysis 13:173–180

    Article  CAS  Google Scholar 

  40. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Anal Biochem 150:76–85

    Article  CAS  Google Scholar 

  41. Nakane PK, Kawaoi A (1974) J Histochem Cytochem 22:1084–1974

    CAS  Google Scholar 

Download references

Acknowledgment

Ministry of Higher Education, Egypt is gratefully acknowledged for financial support to Mahmoud Labib during his study at the Department of Biotechnology, Lund University.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Lund University, P. O. Box 124, 221 00, Lund, Sweden

    Mahmoud Labib, Martin Hedström & Bo Mattiasson

  2. Microbial Biotechnology Center, Faculty of Pharmacy, Cairo University, Kasr El-Aini, P.O. Box 115 62, Cairo, Egypt

    Mahmoud Labib & Magdy Amin

Authors
  1. Mahmoud Labib
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Martin Hedström
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Magdy Amin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bo Mattiasson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bo Mattiasson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Labib, M., Hedström, M., Amin, M. et al. A capacitive biosensor for detection of staphylococcal enterotoxin B. Anal Bioanal Chem 393, 1539–1544 (2009). https://doi.org/10.1007/s00216-008-2559-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-008-2559-x

Keywords

Search

Navigation