Abstract
A fast liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed to study five endocrine-disrupting compounds (4-n-nonylphenol, bisphenol A, estrone, 17β-estradiol and 17α-ethinylestradiol) in water. Different columns were tested; the chromatographic separation of the analytes was optimized on a Pinnacle DB biphenylic column with a water–acetonitrile gradient elution, which allowed the separation of the selected endocrine-disrupting compounds (EDCs) in less than 6 min. Quantitative analysis was performed in selected reaction monitoring (SRM) mode; two transitions were chosen for each compound, using the most abundant for quantitation. Calibration curves using bisphenol A-d 16 as internal standard were drawn, showing good correlation coefficients (0.9993–0.9998). All figures of merit of the method were satisfactory; limits of detection were in the low pg range for all analytes. The method was then applied to the determination of the analytes in real water samples: to this aim, polar organic chemical integrative samplers (POCIS) were deployed in the influent and in the effluent of a drinking water treatment plant in Liguria (Italy). The EDC level was rather low in the influent and negligible in the outlet, reflecting the expected function of the treatment plant.
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Hill M, Stabile C, Steffen K, Hill A (2002) Environ Pollut 117:295–300
Jobling S, Casey D, Rodgers-Gray T, Schulte-Oehlmann U, Pawlowski S, Baunbeck T, Turner AP, Tyler CR (2003) Aquat Toxicol 65:205–220
Madsen SS, Skovbolling S, Nielsen C, Korsgaard B (2004) Aquat Toxicol 68:109–120
Nice HE, Thorndyke MC, Morritt D, Steele S, Crane M (2000) Mar Pollut Bull 50:491–496
Rogers-Gray TP, Jobling S, Morris S, Kelly C, Kirby S, Janbakhsh A, Harries JE, Waldock MJ, Sumpter JP, Tyler CR (2000) Environ Sci Technol 34:1521–1528
Routledge EJ, Sumpter JP (1996) Environ Toxicol Chem 15:241–248
Halling-Sorensen B, Nielsen SN, Lanzky PF, Ingerslev F, Holten Lutzhoft HC, Jorgensen SE (1998) Chemosphere 36:357–393
Joss A, Andersen H, Ternes T, Richle PR, Siegrist H (2004) Environ Sci Technol 38:3047–3055
Clara M, Kreuzinger N, Strenn B, Gans O, Kroiss H (2005) Water Res 39:97–106
Kuster M, Lopez de Alda MJ, Rodriguez-Mozaz S, Barceló D (2007) In: M Petrovic, D Barceló (Eds), Comprehensive Analytical Chemistry, Elsevier
Kuster M, Azevedo DA, López de Alda MJ, Aquino Neto FR, Barceló D (2009) Environ Int 35:997–1003
Belfroid AC, Van der Horst A, Vethaak AD, Schafer AJ, Rijs GBJ, Wegener J, Cofino WP (1999) Sci Total Environ 225:101–108
Kuch HM, Ballschmiter K (2001) Environ Sci Technol 35:3201–3206
Petrovic M, Sole M, Lopez de Alda MJ, Barcelò D (2002) Environ Toxicol Chem 21:2146–2156
Routledge EJ, Sheahan D, Desbrow C, Brighty GC, Waldock M, Sumpter JP (1998) Environ Sci Technol 32:1559–1565
Ternes TA, Meisenheimer M, McDowell D, Sacher F, Brauch HJ, Haist-Gulde B, Preuss G, Wilme U, Zulei-Seibert N (2002) Environ Sci Technol 36:3855–3863
Benotti MJ, Stanford BD, Wert EC, Snyder SA (2009) Water Res 43:1513–1522
Broséus R, Vincent S, Aboulfadl K, Daneshvar A, Sauvé S, Barbeau B, Prévost M (2009) Water Res 43:4707–4717
Lopez de Alda MJ, Diaz-Cruz S, Petrovic M, Barcelò D (2003) J Chromatogr A 1000:503–526
Bacaloni A, Cavaliere C, Faberi A, Foglia P, Samperi R, Lagana A (2005) Anal Chim Acta 531:229–237
Gallart-Ayala H, Moyano E, Galceran MT (2009) Mass Spectrom Rev doi:10.1002/mas.20234
Careri M, Elviri L, Mangia A, Zagnoni I (2003) Chromatographia 57:321–327
Alvarez DA, Petty JD, Huckins JN, Jones-Lepp TL, Getting DT, Goddard JP, Manahan SE (2004) Environ Toxicol Chem 23:1640–1648
Alvarez DA, Stackelberg PE, Petty JD, Huckins JN, Furlong ET, Zaugg SD, Meyer MT (2005) Chemosphere 61:610–622
Vermeirssen EML, Korner O, Schonenberger R, Suter MJF, Burkhardt-Holm P (2005) Environ Sci Technol 39:8191–8198
Alvarez DA, Huckins JN, Petty JD, Jones-Lepp T, Stuer-Lauridsen F, Getting DT, Goddard JP, Gravell A (2007) In: M Petrovic, D Barceló (Eds), Comprehensive Analytical Chemistry, Elsevier
Zhang Z, Hibberd A, Zhou JL (2008) Anal Chim Acta 607:37–44
Liscio C, Magi E, Di Carro M, Suter MJF, Vermeirssen ELM (2009) Environ Pollut 157:2716–2721
Magi E, Di Carro M, Liscio C (2010) Anal Bioanal Chem doi:101007/s00216-010-3656-1
Inoue K, Kawaguchi M, Funakoshi Y, Nakazawa H (2003) J Chromatogr B 798:17–23
Ballesteros-Gómez A, Rubio S, Pérez-Bendito D (2009) J Chromatogr A 1216:449–469
Inoue K, Yamaguchi A, Wada M, Yoshimura Y, Makino T, Nakazawa H (2001) J Chromatogr B 765:121–126
Shao B, Han H, Hu J, Zhao J, Wu G, Xue Y, Ma Y, Zhang S (2005) Anal Chim Acta 530:245–252
Inoue K, Yoshida S, Nakayama S, Ito R, Okanouchi N, Nakazawa H (2006) Arch Environ Contam Toxicol 51:503–508
Hogenboom AC, Hofman MP, Kok SJ, Niessen WMA, Brinkman UAT (2000) J Chromatogr A 892:379–390
Choi BK, Hercules DM, Gusev AI, Fresenius J (2001) Anal Chem 369:370–377
Hu J, Zhang H, Chang H (2005) J Chromatogr A 1070:221–224
Benijts T, Dams R, Gunther W, Lambert W, De Leenheer A (2002) Rapid Commun Mass Sp 16:1358–1364
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Di Carro, M., Scapolla, C., Liscio, C. et al. Development of a fast liquid chromatography–tandem mass spectrometry method for the determination of endocrine-disrupting compounds in waters. Anal Bioanal Chem 398, 1025–1034 (2010). https://doi.org/10.1007/s00216-010-3985-0
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DOI: https://doi.org/10.1007/s00216-010-3985-0