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Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV–visible absorbance

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Abstract

Distinguishing the toxic effects of nanoparticles (NPs) themselves from the well-studied toxic effects of their ions is a critical but challenging measurement for nanotoxicity studies and regulation. This measurement is especially difficult for silver NPs (AgNPs) because in many relevant biological and environmental solutions, dissolved silver forms AgCl NPs or microparticles. Simulations predict that solid AgCl particles form at silver concentrations greater than 0.18 and 0.58 μg/mL in cell culture media and moderately hard reconstituted water (MHRW), respectively. The AgCl NPs are usually not easily separable from AgNPs. Therefore, common existing total silver techniques applied to measure AgNP dissolution, such as inductively coupled plasma mass spectrometry (ICP-MS) or atomic absorption, cannot accurately measure the amount of silver remaining in AgNP form, as they cannot distinguish Ag oxidation states. In this work, we introduce a simple localized surface plasmon resonance (LSPR) UV–visible absorbance measurement as a technique to measure the amount of silver remaining in AgNP form for AgNPs with constant agglomeration states. Unlike other existing methods, this absorbance method can be used to measure the amount of silver remaining in AgNP form even in biological and environmental solutions containing chloride because AgCl NPs do not have an associated LSPR absorbance. In addition, no separation step is required to measure the dissolution of the AgNPs. After using ICP-MS to show that the area under the absorbance curve is an accurate measure of silver in AgNP state for unagglomerating AgNPs in non-chloride-containing media, the absorbance is used to measure dissolution rates of AgNPs with different polymer coatings in biological and environmental solutions. We find that the dissolution rate decreases at high AgNP concentrations, 5 kDa polyethylene glycol thiol coatings increase the dissolution rate, and the rate is much higher in cell culture media than in MHRW.

 

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References

  1. Prabhu BM, Ali SF, Murdock RC, Hussain SM, Srivatsan M (2010) Copper nanoparticles exert size and concentration dependent toxicity on somatosensory neurons of rat. Nanotoxicology 4:150–160

    Article  CAS  Google Scholar 

  2. Unrine JM, Tsyusko OV, Hunyadi SE, Judy JD, Bertsch PM (2010) Effects of particle size on chemical speciation and bioavailability of copper to earthworms (Eisenia fetida) exposed to copper nanoparticles. J Environ Qual 39:1942–1953

    Article  CAS  Google Scholar 

  3. Sotiriou GA, Pratsinis SE (2010) Antibacterial activity of nanosilver ions and particles. Environ Sci Technol 44:5649–5654

    Article  CAS  Google Scholar 

  4. Yeo MK, Yoon JW (2009) Comparison of the effects of nano-silver antibacterial coatings and silver ions on zebrafish embryogenesis. Mol Cell Toxicol 5:23–31

    Google Scholar 

  5. Miura N, Shinohara Y (2009) Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. Biochem Biophys Res Commun 390:733–737

    Article  CAS  Google Scholar 

  6. Liu JY, Hurt RH (2010) Ion release kinetics and particle persistence in aqueous nano-silver colloids. Environ Sci Technol 44:2169–2175

    Article  CAS  Google Scholar 

  7. Sopjani M, Foller M, Haendeler J, Gotz F, Lang F (2009) Silver ion-induced suicidal erythrocyte death. J Appl Toxicol 29:531–536

    Article  CAS  Google Scholar 

  8. Park EJ, Yi J, Kim Y, Choi K, Park K (2010) Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicol Vitr 24:872–878

    Article  CAS  Google Scholar 

  9. Lee J, Ji K, Kim J, Park C, Lim KH, Yoon TH, Choi K (2010) Acute toxicity of two CdSe/ZnSe quantum dots with different surface coating in Daphnia magna under various light conditions. Environ Toxicol 25:593–600

    Article  CAS  Google Scholar 

  10. Poynton HC, Lazorchak JM, Impellitteri CA, Smith ME, Rogers K, Patra M, Hammer KA, Allen HJ, Vulpe CD (2010) Differential gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions. Environ Sci Technol 45:762–768

    Article  Google Scholar 

  11. Zhang LW, Monteiro-Riviere NA (2009) Mechanisms of quantum dot nanoparticle cellular uptake. Toxicol Sci 110:138–155

    Article  CAS  Google Scholar 

  12. Lubick N (2008) Nanosilver toxicity: ions, nanoparticles or both? Environ Sci Technol 42:8617

    Article  CAS  Google Scholar 

  13. Limbach LK, Grass RN, Stark WJ (2009) Physico-chemical differences between particle- and molecule-derived toxicity: can we make inherently safe nanoparticles? Chimia 63:38–43

    Article  CAS  Google Scholar 

  14. Hussain SM, Braydich-Stolle LK, Schrand AM, Murdock RC, Yu KO, Mattie DM, Schlager JJ, Terrones M (2009) Toxicity evaluation for safe use of nanomaterials: recent achievements and technical challenges. Adv Mater 21:1549–1559

    Article  CAS  Google Scholar 

  15. Kennedy AJ, Hull MS, Bednar AJ, Goss JD, Gunter JC, Bouldin JL, Vikesland PJ, Steevens JA (2010) Fractionating nanosilver: importance for determining toxicity to aquatic test organisms. Environ Sci Technol 44:9571–9577

    Article  CAS  Google Scholar 

  16. Elzey S, Grassian VH (2010) Agglomeration, isolation and dissolution of commercially manufactured silver nanoparticles in aqueous environments. J Nanoparticle Res 12:1945–1958

    Article  CAS  Google Scholar 

  17. Liu JY, Sonshine DA, Shervani S, Hurt RH (2010) Controlled release of biologically active silver from nanosilver surfaces. ACS Nano 4:6903–6913

    Article  CAS  Google Scholar 

  18. Kittler S, Greulich C, Diendorf J, Koller M, Epple M (2010) Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions. Chem Mater 22:4548–4554

    Article  CAS  Google Scholar 

  19. Haase A, Arlinghaus HF, Tentschert J, Jungnickel H, Graf P, Mantion A, Draude F, Galla S, Plendl J, Goetz ME, Masic A, Meier W, Thuenemann AF, Taubert A, Luch A (2011) Application of laser postionization secondary neutral mass spectrometry/time-of-flight secondary ion mass spectrometry in nanotoxicology: visualization of nanosilver in human macrophages and cellular responses. ACS Nano 5:3059–3068

    Article  CAS  Google Scholar 

  20. MacCuspie RI, Allen AJ, Hackley VA (2011) Dispersion stabilization of silver nanoparticles in synthetic lung fluid studied under in situ conditions. Nanotoxicology 5:140–156

    Article  Google Scholar 

  21. Stebounova L, Guio E, Grassian V (2011) Silver nanoparticles in simulated biological media: a study of aggregation, sedimentation, and dissolution. J Nanoparticle Res 13:233–244

    Article  CAS  Google Scholar 

  22. MacCuspie RI (2011) Colloidal stability of silver nanoparticles in biologically relevant conditions. J Nanoparticle Res 13:2893–2908

    Article  CAS  Google Scholar 

  23. Zook JM, MacCuspie RI, Locascio LE, Halter MD, Elliott JT (2011) Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity. Nanotoxicology. doi:10.3109/17435390.17432010.17536615

  24. Hackley VA, Clogston JD (2007) NIST-NCL joint assay protocol PCC-1: measuring the size of nanoparticles in aqueous media using batch-mode dynamic light scattering. Available at http://ncl.cancer.gov/working_assay-cascade.asp

  25. ISO (1993) Guide to the expression of uncertainty in measurement, 1st edn. ISO, Geneva

    Google Scholar 

  26. USEPA (2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms. 821/R-802-012. United States Environmental Protection Agency, Washington

  27. Shaff JE, Schultz BA, Craft EJ, Clark RT, Kochian LV (2010) GEOCHEM-EZ: a chemical speciation program with greater power and flexibility. Plant and Soil 330:207–214

    Article  CAS  Google Scholar 

  28. DECHEMA (1992) Handbook of corrosion. VCH Publishers, Weinheim

    Google Scholar 

  29. Tsai D-H, DelRio FW, Keene AM, Tyner KM, MacCuspie RI, Cho TJ, Zachariah MR, Hackley VA (2011) Adsorption and conformation of serum albumin protein on gold nanoparticles investigated using dimensional measurements and in situ spectroscopic methods. Langmuir 27:2464–2477

    Article  CAS  Google Scholar 

  30. Tsai D-H, DelRio FW, MacCuspie RI, Cho TJ, Zachariah MR, Hackley VA (2010) Competitive adsorption of thiolated polyethylene glycol and mercaptopropionic acid on gold nanoparticles measured by physical characterization methods. Langmuir 26:10325–10333

    Article  CAS  Google Scholar 

  31. Bland JM, Altman DG (1986) Statistical-methods for assessing agreement between 2 methods of clinical measurement. Lancet 1:307–310

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Dr. MS 8313, Gaithersburg, MD, 20899, USA

    Justin M. Zook, Stephen E. Long, Danielle Cleveland, Carly Lay A. Geronimo & Robert I. MacCuspie

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  1. Justin M. Zook
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  2. Stephen E. Long
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  3. Danielle Cleveland
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  4. Carly Lay A. Geronimo
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  5. Robert I. MacCuspie
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Correspondence to Justin M. Zook.

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Zook, J.M., Long, S.E., Cleveland, D. et al. Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV–visible absorbance. Anal Bioanal Chem 401, 1993–2002 (2011). https://doi.org/10.1007/s00216-011-5266-y

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  • DOI: https://doi.org/10.1007/s00216-011-5266-y

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