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Preparation of Fe3O4@C@CNC multifunctional magnetic core/shell nanoparticles and their application in a signal-type flow-injection photoluminescence immunosensor

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Abstract

We describe here the preparation of carbon-coated Fe3O4 magnetic nanoparticles that were further fabricated into multifunctional core/shell nanoparticles (Fe3O4@C@CNCs) through a layer-by-layer self-assembly process of carbon nanocrystals (CNCs). The nanoparticles were applied in a photoluminescence (PL) immunosensor to detect the carcinoembryonic antigen (CEA), and CEA primary antibody was immobilized onto the surface of the nanoparticles. In addition, CEA secondary antibody and glucose oxidase were covalently bonded to silica nanoparticles. After stepwise immunoreactions, the immunoreagent was injected into the PL cell using a flow-injection PL system. When glucose was injected, hydrogen peroxide was obtained because of glucose oxidase catalysis and quenched the PL of the Fe3O4@C@CNC nanoparticles. The here proposed PL immunosensor allowed us to determine CEA concentrations in the 0.005–50 ng · mL-1 concentration range, with a detection limit of 1.8 pg · mL-1.

The Fe3O4@C@CNC was prepared and applied in a CEA immunosensor with the help of a flow-injection photoluminescence system.

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Acknowledgments

This work was financially supported by the Natural Science Research Foundation of China (51273084, 51003039, 21207048, 21277058), the Natural Science Foundation of Shandong Province, China (ZR2012BZ002), and the Technology Development Plan of Shandong Province, China (grants 2012GGB01181 and 2011GGB01153).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Chengchao Chu, Meng Li, Long Li, Lei Ge, Jinghua Yu & Mei Yan

  2. Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, China

    Shenguang Ge

  3. Cancer Research Center, Shandong Tumor Hospital, Jinan, 250012, China

    Xianrang Song

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  1. Chengchao Chu
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  2. Meng Li
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  3. Long Li
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  4. Shenguang Ge
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  5. Lei Ge
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  6. Jinghua Yu
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  7. Mei Yan
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Correspondence to Mei Yan.

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Chu, C., Li, M., Li, L. et al. Preparation of Fe3O4@C@CNC multifunctional magnetic core/shell nanoparticles and their application in a signal-type flow-injection photoluminescence immunosensor. Anal Bioanal Chem 405, 9555–9561 (2013). https://doi.org/10.1007/s00216-013-7356-5

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  • DOI: https://doi.org/10.1007/s00216-013-7356-5

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