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Rapid quantification method for Legionella pneumophila in surface water

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Abstract

World-wide legionellosis outbreaks caused by evaporative cooling systems have shown that there is a need for rapid screening methods for Legionella pneumophila in water. Antibody-based methods for the quantification of L. pneumophila are rapid, non-laborious, and relatively cheap but not sensitive enough for establishment as a screening method for surface and drinking water. Therefore, preconcentration methods have to be applied in advance to reach the needed sensitivity. In a basic test, monolithic adsorption filtration (MAF) was used as primary preconcentration method that adsorbs L. pneumophila with high efficiency. Ten-liter water samples were concentrated in 10 min and further reduced to 1 mL by centrifugal ultrafiltration (CeUF). The quantification of L. pneumophila strains belonging to the monoclonal subtype Bellingham was performed via flow-based chemiluminescence sandwich microarray immunoassays (CL-SMIA) in 36 min. The whole analysis process takes 90 min. A polyclonal antibody (pAb) against L. pneumophila serogroup 1–12 and a monoclonal antibody (mAb) against L. pneumophila SG 1 strain Bellingham were immobilized on a microarray chip. Without preconcentration, the detection limit was 4.0 × 103 and 2.8 × 103 CFU/mL determined by pAb and mAb 10/6, respectively. For samples processed by MAF-CeUF prior to SMIA detection, the limit of detection (LOD) could be decreased to 8.7 CFU/mL and 0.39 CFU/mL, respectively. A recovery of 99.8 ± 15.9 % was achieved for concentrations between 1–1000 CFU/mL. The established combined analytical method is sensitive for rapid screening of surface and drinking water to allow fast hygiene control of L. pneumophila.

Combined process with MAF, CeUF and CL-SMIA for the rapid detection of L. pneumophila in surface water

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References

  1. Muder RR, Yu VL, Woo AH. Arch Intern Med. 1986;146:1607–12.

    Article  CAS  Google Scholar 

  2. Atlas RM. Environ Microbiol. 1999;61:283–93.

    Article  Google Scholar 

  3. Rangel KM, Delclos G, Emery R, Symanski E. J Occup Environ Hyg. 2011;8:249–65.

    Article  Google Scholar 

  4. Fields BS, Benson RF, Besser RE. Clin Microbiol Rev. 2002;15:506–26.

    Article  Google Scholar 

  5. Fliermans C, Cherry W, Orrison L, Smith S, Tison D, Pope D. Appl Environ Microbiol. 1981;41:9–16.

    CAS  Google Scholar 

  6. Brooks T, Osicki RA, Springthorpe VS, Sattar SA, Filion L, Abrial D, et al. J Toxicol Environ Health A. 2004;67:1845–59.

    Article  CAS  Google Scholar 

  7. Abdel-Nour M, Duncan C, Low DE, Guyard C. Int J Mol Sci. 2013;14:21660–75.

    Article  Google Scholar 

  8. van Heijnsbergen E, Schalk JAC, Euser SM, Brandsema PS, den Boer JW, Husman AMD. Environ Sci Technol. 2015;49:4797–815.

    Article  Google Scholar 

  9. Helbig JM, Kurtz JB, Pastoris MC, Pelaz C, Lück PC. J Clin Microbiol. 1997;35:2841–5.

    CAS  Google Scholar 

  10. Joly JR, McKinney RM, Tobin JO, Bibb WF, Watkins ID, Ramsay D. J Clin Microbiol. 1986;23:76–771.

    Google Scholar 

  11. Kunze A, Pei L, Elsaesser D, Niessner R, Seidel M. J Virol Methods. 2015;222:132–7.

    Article  CAS  Google Scholar 

  12. Wolter A, Niessner R, Seidel M. Anal Chem. 2008;80:5854–63.

    Article  CAS  Google Scholar 

  13. Kloth K, Niessner R, Seidel M. Biosens Bioelectron. 2009;24:2106–12.

    Article  CAS  Google Scholar 

  14. Lengger S, Otto J, Elsaesser D, Schneider O, Tiehm A, Fleischer J, et al. Anal Bioanal Chem. 2014;406:3323–34.

    Article  CAS  Google Scholar 

  15. Trinkwasserverordnung. Bundesministerium der Justiz und für Verbraucherschutz, Berlin.2011.

  16. OSHA. OSHA technical manual section III: chapter 7 Legionnaires’ disease. Washington, DC: Occupational Safety and Health Administration of the US Department of Labor; 1999.

    Google Scholar 

  17. Lee JV, Lai S, Exner M, Lenz J, Gaia V, Casati S, et al. J Appl Microbiol. 2011;110:1032–44.

    Article  CAS  Google Scholar 

  18. Fuchslin HP, Kotzsch S, Keserue HA, Egli T. Cytometry. 2010;77:264–74.

    Google Scholar 

  19. Keserue HA, Baumgartner A, Felleisen R, Egli T. Microb Biotechnol. 2012;5:753–63.

    Article  Google Scholar 

  20. Shimada T, Noguchi Y, Jackson JL, Miyashita J, Hayashino Y, Kamiya T, et al. Chest J. 2009;136:1576–85.

    Article  Google Scholar 

  21. Dennis PJL, Wright AE, Rutter DA, Death JE, Jones BPC. J Hyg Cambridge. 1984;93:34–353.

    Article  Google Scholar 

  22. Peskoller C, Niessner R, Seidel M. J Chromatogr A. 2009;1216:3794–801.

    Article  CAS  Google Scholar 

  23. Karsunke XY, Niessner R, Seidel M. Anal Bioanal Chem. 2009;395:1623–30.

    Article  CAS  Google Scholar 

  24. Langer V, Hartmann G, Niessner R, Seidel M. J Aerosol Sci. 2012;48:46–55.

    Article  CAS  Google Scholar 

  25. Wutz K, Meyer VK, Wachek S, Krol P, Gareis M, Nölting C, et al. Anal Chem. 2013;85:5279–85.

    Article  CAS  Google Scholar 

  26. Blatny JM, Reif BA, Skogan G, Andreassen O, Høiby EA, Ask E, et al. Environ Sci Technol. 2008;42:7360–7.

    Article  CAS  Google Scholar 

  27. Leoni E, Legnani P. J Appl Microbiol. 2001;90:27–33.

    Article  CAS  Google Scholar 

  28. Wery N, Bru-Adan V, Minervini C, Delgenes JP, Garrelly L, Godon JJ. Appl Environ Microbiol. 2008;74:3030–7.

    Article  CAS  Google Scholar 

  29. Kirschner AK, Rameder A, Schrammel B, Indra A, Farnleitner AH, Sommer R. J Appl Microbiol. 2012;112:1244–56.

    Article  CAS  Google Scholar 

Download references

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Correspondence to Michael Seidel.

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Wunderlich, A., Torggler, C., Elsässer, D. et al. Rapid quantification method for Legionella pneumophila in surface water. Anal Bioanal Chem 408, 2203–2213 (2016). https://doi.org/10.1007/s00216-016-9362-x

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  • DOI: https://doi.org/10.1007/s00216-016-9362-x

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