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Extraction of sage (Salvia officinalis L.) by pressurized hot water and conventional methods: antioxidant activity of the extracts

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Abstract.

The effectiveness of the extraction of antioxidative compounds from sage (Salvia officinalis L.) by pressurized hot water extraction, ultrasonication-assisted methanol extraction, hydrodistillation, and maceration with 70% ethanol was evaluated by determining the capability of the extracts to scavenge the free radical DPPH· (1,1-diphenyl-2-picrylhydrazyl) in vitro. Pressurized hot water extraction was found to be the most effective extraction procedure, followed by maceration with 70% ethanol, hydrodistillation, and ultrasonication-assisted methanol extraction. In addition to the total extract, special attention was paid to rosmarinic and carnosic acids, carnosol and methyl carnosate. The extracts were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). The identification of compounds was confirmed by coupling RP-HPLC to mass spectrometry with electrospray ionization.

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Authors and Affiliations

  1. Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FIN-00014 University of Helsinki, Finland, , , , ,

    Maarit Ollanketo, Kari Hartonen & Marja-Liisa Riekkola

  2. Division of Pharmacognosy, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Finland, , , , ,

    Anna Peltoketo & Raimo Hiltunen

Authors
  1. Maarit Ollanketo
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  2. Anna Peltoketo
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  3. Kari Hartonen
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  4. Raimo Hiltunen
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  5. Marja-Liisa Riekkola
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Ollanketo, M., Peltoketo, A., Hartonen, K. et al. Extraction of sage (Salvia officinalis L.) by pressurized hot water and conventional methods: antioxidant activity of the extracts. Eur Food Res Technol 215, 158–163 (2002). https://doi.org/10.1007/s00217-002-0545-7

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  • DOI: https://doi.org/10.1007/s00217-002-0545-7

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