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Optimization of culture medium for yellow pigments production with Monascus anka mutant using response surface methodology

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Abstract

In our laboratory, one Monascus anka mutant able to produce high yield of yellow pigments screened by physical and chemical combination mutagenesis was obtained. This study evaluated peptone, NH4NO3 and KH2PO4 as the most significant variables for Monascus yellow pigment production by this M. anka mutant MYM in submerged fermentation. Response surface methodology (RSM) optimized these nutrient parameters for maximum yellow pigment production (87.24 OD units), which resulted at 10.3 g/L peptone, 11.9 g/L NH4NO3 and 4.7 g/L KH2PO4 in the medium. According to the fitting equation, through five replication experiments under the optimized conditions, the average yellow pigment production obtained was 88.14 OD units for flask cultivation and 92.45 OD units for 5 L fermenter cultivation. Meanwhile, the citrinin could not be detected by HPLC method.

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Acknowledgments

One of authors (Bo Zhou) thank Ph.D. Yuan for editing this manuscript.

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Panyu Campus city, Guangzhou, 510006, People’s Republic of China

    Bo Zhou, Jufang Wang, Yuewu Pu, Mingjun Zhu & Shizhong Liang

  2. Department of Plant Soil and Agricultural System, Southern Illinois University, 1205 Lincoln Drive, Carbondale, IL, 62901, USA

    Shiming Liu

Authors
  1. Bo Zhou
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  2. Jufang Wang
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  3. Yuewu Pu
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  4. Mingjun Zhu
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  5. Shiming Liu
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  6. Shizhong Liang
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Correspondence to Shizhong Liang.

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Zhou, B., Wang, J., Pu, Y. et al. Optimization of culture medium for yellow pigments production with Monascus anka mutant using response surface methodology. Eur Food Res Technol 228, 895–901 (2009). https://doi.org/10.1007/s00217-008-1002-z

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  • DOI: https://doi.org/10.1007/s00217-008-1002-z

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