Abstract
Histamine levels in samples of fish and marine products were determined by capillary zone electrophoresis using phosphate buffer at pH 2.44 and UV detection at 214 nm. A plot of the area of the peaks versus histamine concentration was linear over the range of 1–100 ppm with a correlation coefficient of 0.9996. Calibration using the height of the peak also gave good correlation, with a correlation coefficient of 0.9969, at concentrations between 1 ppm and 20 ppm. The recovery of added histamine in different samples of fish and fish products was, on average, 99.65%. The method described in this work was used for the quantitation of histamine in fish and fish products.
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Received: 19 March 1996/Revised version: 22 July 1996
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Gallardo, J., Sotelo, C. & Perez-Martin, R. Determination of histamine by capillary zone electrophoresis using a low-pH phosphate buffer: application in the analysis of fish and marine products. Z Lebensm Unters Forsch 204, 336–340 (1997). https://doi.org/10.1007/s002170050086
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DOI: https://doi.org/10.1007/s002170050086