Abstract
Nucleic acid-based species identification often targets the mitochondrial encoded cytb gene. However, polymerase chain reaction (PCR)- restriction fragment length polymorphism (RFLP) analysis using universal primers sometimes leads to ambiguous results, which are due to the presence of nuclear encoded pseudo-cytb genes. Such ambiguities were succesfully avoided using a newly developed method for the enrichment of mitochondrial DNA. In addition, a mitochondrial cytb-specific PCR system was designed allowing the unambiguous identification of game meat.
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Received: 24 February 1998
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Burgener, M., Hübner, P. Mitochondrial DNA enrichment for species identification and evolutionary analysis. Z Lebensm Unters Forsch 207, 261–263 (1998). https://doi.org/10.1007/s002170050329
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DOI: https://doi.org/10.1007/s002170050329