Abstract
In the methylated form, mercury represents a concern to public health primarily through the consumption of contaminated fish tissue. Research conducted on the methylation of mercury strongly suggests that the process is microbial in nature and facilitated principally by sulfate-reducing bacteria. This study addressed the potential for mercury methylation by varying sulfate treatments and wetland-based soil in microbial slurry reactors with available inorganic mercury. Under anoxic laboratory conditions conducive to the growth of naturally occurring sulfate-reducing bacteria in the soil, it was possible to evaluate how various sulfate additions influenced the methylation of inorganic mercury added to overlying water as well as the sequestration of dissolved copper. Treatments included sulfate amendments ranging from 25 to 500 mg/L (0.26 to 5.2 mM) above the soil’s natural sulfate level. Mercury methylation in sulfate treatments did not exceed that of the nonamended control during a 35-day incubation period. However, increases in methylmercury concentration were linked to bacterial growth and sulfate reduction. A time lag in methylation in the highest treatment correlated with an equivalent lag in bacterial growth. The decrease in dissolved copper ranged from 72.7% in the control to 99.7% in the highest sulfate treatment. It was determined that experimental systems such as these can provide some useful information but that they also have severe limitations once sulfate is depleted or if sulfate is used in excess.
Similar content being viewed by others
References
American Public Health Association (1985) Standard methods for the examination of water and wastewater, 16th ed. Part 900—Microbiological examination of water. APH Association. Washington, DC
Beijer K, Jernelov A (1979) Methylation of mercury in aquatic environments In: Nriagu JO (ed) The biogeochemistry of Mercury in the environment. Elsevier/North-Holland, Amsterdam, The Netherlands, pp 203–210
Benoit JM, Gilmour CC, Mason RP (2001a) Aspects of bioavailability of mercury for methylation in pure cultures of Desulfobulbus propionicus (1pr3). Appl Environ Microbiol 67:51–58
Benoit JM, Gilmour CC, Mason RP (2001b) The influence of sulfide on solid-phase mercury bioavailability for methylation by pure cultures of Desulfobulbus propionicus (1pr3). Environ Sci Technol 35:127–132
Benoit JM, Gilmour CC, Mason RP, Heyes A (1999a) Sulfide controls on mercury speciation and bioavailability to methylating bacteria in sediment pore waters. Environ Sci Technol 33:951–957
Benoit JM, Gilmour CC, Mason RP, Riedel GS, Riedel GF (1998) Behavior of mercury in the Patuxent River Estuary. Biogeochemistry 40:249–265
Benoit JM, Mason RP, Gilmour CC (1999b) Estimation of mercury-sulfide speciation in sediment pore waters using octanol water partitioning and implications for availability to methylating bacteria. Environ Toxicol Chem 18:2138–2141
Bloom NS (1989) Determination of picogram levels of methylmercury by aqueous phase ethylation, followed by cryogenic gas chromatography with cold vapor atomic fluorescence detection. Can J Fish Aquat Sci 46:1131–1140
Bloom NS, Gill GA, Cappellino S, Dobbs C, McShea L, Driscoll C, et al. (1999) Speciation and cycling of mercury in Lavaca Bay, Texas sediments. Environ Sci Technol 33:7–13
Bloom NS, Fitzgerald WF (1988) Determination of volatile mercury species at the picogram level by low temperature gas chromatography with cold vapor atomic fluorescence detection. Anal Chim Acta 208:151–161
Blum JE, Bartha R (1980) Effects of salinity on methylation of mercury. Bull Environ Contam Toxicol 25:404–408
Bodaly RA, St. Louis VL, Paterson MJ, Fudge RJP, Hall BD, Rosenberg DM, et al. (1997) Bioaccumulation of mercury in the aquatic food chain in newly flooded areas. In: Sigel A, Sigel H (eds) Metal ions in biological system. Volume 34. Mercury and its effects on environment and biology. Dekker New York, NY, pp 259–287
Compeau G, Bartha R (1985) Sulfate reducing bacteria: Principal methylators of mercury in anoxic estuarine sediments. Appl Environ Microbiol 50:498–502
Craig PJ, Moreton PA (1983) Total mercury, methylmercury, and sulfide in River Carron sediments. Mar Pollut Bull 14:408–411
Dvorak DH, Hedin RS, Edenborn HM, McIntire PE (1992) Treatment of metal contaminated water using bacterial sulfate reduction: Results from pilot-scale reactors. Biotechnol Bioeng 40:609–616
Feng J, Hsieh YP (1998) Sulfate reduction in freshwater wetland soils and the effects of sulfate and substrate loading. J Environ Qual 27:968–972
Gilmour CG, Capone DG (1987) Relationship between Hg methylation and the sulfur cycle in estuarine sediments. EOS 68:1718
Gilmour CG, Henry EA (1991) Mercury methylation in aquatic systems affected by acid deposition. Environ Pollut 71:131–169
Gilmour CG, Henry EA, Mitchell R (1992) Sulfate stimulation of mercury methylation in freshwater sediments. Environ Sci Technol 26:2281–2287
Gilmour CG, Riedel GS, Ederington MC, Bell JT, Benoit JM, Gill GA, et al. (1998) Methylmercury concentration and production rates across a trophic gradient in the northern Everglades. Biogeochemistry 40:327–345
Harmon SM, King JK, Gladden JB, Chandler GT, Newman LA (2004) Methylmercury formation in a wetland mesocosm amended with sulfate. Environ Sci Technol 38:650–656
Harmon SM, King JK, Gladden JB, Chandler GT, Newman LA (2005) Mercury body burdens in Gambusia holbrooki and Erimyzon sucetta from sulfate-amended wetland mesocosm. Chemosphere 59:227–233
Horvat M, Bloom NS, Liang L (1993) Comparison of distillation with other current isolation methods for the determination of methylmercury compounds in low level environmental samples. Part I. Sediments. Anal Chim Acta 281:135–152
Kelly CA, Rudd JWM, St Louis VL, Heyes A (1995) Is total mercury concentration a good predictor of methyl mercury concentration in aquatic systems. Water Air Soil Pollut 80:715–724
King JK, Harmon SM, Fu TT, Gladden JB (2002) Mercury removal, methylmercury formation, and sulfate reducing bacteria profiles in wetland mesocosms. Chemosphere 46:859–870
King JK, Kostka JE, Frischer ME, Saunddrs FM, Jahnke RA (2001) A quantitative relationship that demonstrates mercury methylation rates in marine sediments are based on the community composition and activity of sulfate-reducing bacteria. Environ Sci Technol 35:2491–2496
King JK, Saunders FM, Lee RF, Jahnke RA (1999) Coupling mercury methylation rates to sulfate reduction rates in marine sediments. Environ Toxicol Chem 18:1362–1369
Krabbenhoft DP, Hurley JP, Olson ML, Cleckner LB (1998) Diel variability of mercury phases and species distributions in the Florida Everglades. Biogeochemistry 40:311–325
Lehman. RW, Mooney FD, Rodgers JH Jr, Gladden JB, Murray-Guide C, Bell JF (2002) Wetlands for industrial wastewater treatment at the Savannah River site. Technical paper 0202. National Defense Industrial Association 28th Environmental & Energy Symposium, Charleston, SC, March 2002
Liang L, Horvat M, Bloom NS (1994) An improved speciation method for mercury by GC/CVAFS after aqueous phase ethylation and room temperature precollection. Talanta 41:371–379
National Research Council (2000) Toxicological effects of methylmercury. National Academy Press, Washington, DC
Pak KR, Bartha R (1998) Mercury methylation and demethylation in anoxic lake sediments and by strictly anaerobic bacteria. Appl Environ Microbiol 64:1013–1017
Stamenkovic J, Gustin MS, Dennett KE (2005) Net methyl mercury production versus water quality improvement in constructed wetlands: Trade-offs in pollution control. Wetlands 25:748–757
United States Environmental Protection Agency (1997) Mercury Report to Congress. Office of Air Quality and Standards. Washington, DC
United States Environmental Protection Agency (1998) Method 1630: Methyl mercury in water by distillation, aqueous ethylation, purge and trap, and cold vapor atomic fluorescence spectrometry. August 1998. USEPA, Washington, DC
United States Environmental Production Agency (2004) Update: National listing of fish and wildlife advisories. USEPA fact sheet. EPA-823-F-02-007. Office of Water, Washington, DC. Available at: http://www.epa.gov/waterscience/fish/advisories/factsheet.pdf. Accessed: November 11, 2006
Widdel F, Bak F (1992) Gram-negative mesophilic sulfate-reducing bacteria. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (ed) The prokaryotes, A handbook on the biology of bacteria: Ecophysiology, isolation, identification, applications, 2nd ed. Volume 4. Springer-Verlag, New York, NY, pp 3352–3378
Winfrey MR, Rudd JWM (1990) Environmental factors affecting the formation of methylmercury in low-pH lakes: Review. Environ Toxicol Chem 9:853–870
Acknowledgments
This work was supported by the United States Department of Energy and Westinghouse Savannah River Company through a research program administered by the Oak Ridge Institute for Science and Education. The authors thank D. Wells, J. Bowers, R. Ray, M. Jones, D. Addis, P. Mckinsey, and C. Turick for analytical and technical assistance. All mercury samples were analyzed at the Skidaway Institute of Oceanography, Savannah, GA.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Harmon, S.M., King, J.K., Gladden, J.B. et al. Using Sulfate-Amended Sediment Slurry Batch Reactors to Evaluate Mercury Methylation. Arch Environ Contam Toxicol 52, 326–331 (2007). https://doi.org/10.1007/s00244-006-0071-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00244-006-0071-x