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Characterization of Vibrios Diversity in the Mucus of the Polychaete Myxicola infundibulum (Annellida, Polichaeta)

  • Invertebrate Microbiology
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Abstract

Vibrios are among the most abundant culturable microbes in aquatic environments. They can be either free-living in the water column or associated with several marine organisms as mutualists, saprophytes, or parasites. In the present study we analysed vibrios abundance and diversity in the mucus of the polychaete Myxicola infundibulum, complementing culture-based with molecular methods. Vibrios reached 4.6 × 103 CFU mL−1 thus representing a conspicuous component of the heterotrophic culturable bacteria. In addition, luminous vibrios accounted for about 60 % of the total culturable vibrios in the mucus. The isolates were assigned to: Vibrio gigantis, Vibrio fischeri, Vibrio jasicida, Vibrio crassostreae, Vibrio kanaloae, and Vibrio xuii. Two Vibrio isolates (MI-13 and MI-15) may belong to a new species. We also tested the ability of the Vibrio isolates to grow on M. infundibulum mucus as the sole carbon source. All strains showed appreciable growth in the presence of mucus, leading us to conclude that this matrix, which is abundant and covers the animal entirely, may represent a microcosm and a food source for some bacteria, playing a crucial role in the structuring of a mucus-associated beneficial microbial community. Moreover, the trophic relationship between vibrios and M. infundibulum mucus could be enhanced by the protection that mucus offers to vibrios. The results of this study represent a contribution to the growing evidence for complex and dynamic invertebrate-microbe associations present in nature and highlight the importance of exploring relationships that Vibrio species establish with marine invertebrates.

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Acknowledgements

Financial support was provided by the PRIN Project (2010–2011) and RITMARE Flagship Project both funded by the Italian Ministry of University and Research.

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  1. Istituto per l’Ambiente Marino Costiero, U.O.S. di Taranto, CNR, Via Roma 3, Taranto, Italy

    Loredana Stabili

  2. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, Lecce, Italy

    Loredana Stabili, Graziano Pizzolante, Giorgia Caruso & Pietro Alifano

  3. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, CoNISMa Unità di Lecce, Via Prov.le Lecce Monteroni, Lecce, Italy

    Adriana Giangrande

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  1. Loredana Stabili
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Stabili, L., Giangrande, A., Pizzolante, G. et al. Characterization of Vibrios Diversity in the Mucus of the Polychaete Myxicola infundibulum (Annellida, Polichaeta). Microb Ecol 67, 186–194 (2014). https://doi.org/10.1007/s00248-013-0312-2

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