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Improved xylanase production by Trichoderma reesei grown on l-arabinose and lactose or d-glucose mixtures

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Abstract

Trichoderma reesei Rut C-30 was grown on eight different natural or rare aldopentoses as the main carbon source and on mixtures of an aldopentose with d-glucose or lactose. The fungal cells consumed all aldopentoses tested, except l-xylose and l-ribose. The highest total xylanase and cellulase activities were achieved when cells were grown on l-arabinose as the main carbon source. The total xylanase activity produced by cells grown on l-arabinose was even higher than that produced by cells grown on an equal amount of lactose. In co-metabolism of d-glucose (15 g l−1) and l-arabinose (5 g l−1), the total volumetric and specific xylanase productivities were improved (derepressed) approximately 23- and 18-fold, respectively, compared to a cultivation on only d-glucose (20 g l−1). In a similar experiment, in which cells were grown on a mixture of lactose and l-arabinose, the xylanase productivity was approximately doubled, compared to a cultivation on only lactose. The cellulase productivities, however, were not improved by the addition of l-arabinose. Compared with a typical industrial fungal enzyme production process with lactose as the main carbon source, better volumetric and specific xylanase productivities were achieved both on a lactose/arabinose mixture and on a glucose/arabinose mixture.

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Acknowledgements

Auli Murrola is acknowledged for HPLC analysis and Seppo Jääskeläinen for technical assistance. Financial support from the Research Foundation of Helsinki University of Technology is gratefully acknowledged.

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Correspondence to N. von Weymarn.

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Xiong, H., Turunen, O., Pastinen, O. et al. Improved xylanase production by Trichoderma reesei grown on l-arabinose and lactose or d-glucose mixtures. Appl Microbiol Biotechnol 64, 353–358 (2004). https://doi.org/10.1007/s00253-003-1548-4

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  • DOI: https://doi.org/10.1007/s00253-003-1548-4

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