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Conversion of agricultural feedstock and coproducts into poly(hydroxyalkanoates)

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Abstract

Aside from their importance to the survival and general welfare of mankind, agriculture and its related industries produce large quantities of feedstocks and coproducts that can be used as inexpensive substrates for fermentative processes. Successful adoption of these materials into commercial processes could further the realization of a biorefinery industry based on agriculturally derived feedstocks. One potential concept is the production of poly(hydroxyalkanoate) (PHA) polymers, a family of microbial biopolyesters with a myriad of possible monomeric compositions and performance properties. The economics for the fermentative production of PHA could benefit from the use of low-cost agricultural feedstocks and coproducts. This mini-review provides a brief survey of research performed in this area, with specific emphasis on studies describing the utilization of intact triacylglycerols (vegetable oils and animal fats), dairy whey, molasses, and meat-and-bone meal as substrates in the microbial synthesis of PHA polymers.

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References

  • Ahn WS, Park SJ, Lee SY (2000) Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrate whey solution. Appl Environ Microbiol 66:3624–3627

    Article  CAS  Google Scholar 

  • Ahn WS, Park SJ, Lee SY (2001) Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli. Biotechnol Lett 23:235–240

    Article  CAS  Google Scholar 

  • Akiyama M, Tsuge T, Doi Y (2003) Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation. Polym Degrad Stab 80:183–194

    Article  CAS  Google Scholar 

  • Alias Z, Tan IKP (2005) Isolation of palm oil-utilising, polyhydroxyalkanoate (PHA)-producing bacteria by an enrichment technique. Bioresour Technol 96:1229–1234

    Article  CAS  Google Scholar 

  • Ashby RD, Foglia TA (1998) Poly(hydroxyalkanoate) biosynthesis from triglyceride substrates. Appl Microbiol Biotechnol 49:431–437

    Article  CAS  Google Scholar 

  • Ashby RD, Solaiman DKY, Foglia TA (2004) Bacterial poly(hydroxyalkanoate) polymer production from the biodiesel co-product stream. J Polym Environ 12:105–112

    Article  CAS  Google Scholar 

  • Ashby RD, Solaiman DKY, Foglia TA (2005) Synthesis of short-/medium-chain-length poly(hydroxyalkanoate) blends by mixed culture fermentation of glycerol. Biomacromolecules 6:2106–2112

    Article  CAS  Google Scholar 

  • Athanasiadis I, Boskou D, Kanellaki M, Kiosseoglou V, Koutinas AA (2002) Whey liquid waste of the dairy industry as raw material for potable alcohol production by kefir granules. J Agric Food Chem 50:7231–7234

    Article  CAS  Google Scholar 

  • Bormann EJ, Roth M (1999) The production of polyhydroxybutyrate by Methylobacterium rhodesianum and Ralstonia eutropha in media containing glycerol and casein hydrolysates. Biotechnol Lett 21:1059–1063

    Article  CAS  Google Scholar 

  • Braunegg G, Bona R, Koller M (2004) Sustainable polymer production. Polym-Plastics Technol Eng 43:1779–1793

    Article  CAS  Google Scholar 

  • Celik GY, Beyatli Y (2005) Determination of poly-beta-hydroxybutyrate (PHB) in sugarbeet molasses by Pseudomonas cepacia G13 strain. Zuckerindustrie 130:201–203

    CAS  Google Scholar 

  • Cromwick A-M, Foglia T, Lenz RW (1996) The microbial production of poly(hydroxyalkanoates) from tallow. Appl Microbiol Biotechnol 46:464–469

    Article  CAS  Google Scholar 

  • Dhanasekar R, Viruthagiri T (2005) Batch kinetics and modeling of poly-β-hydroxybutyrate synthesis from Azotobacter vinelandii using different carbon sources. India J Chem Technol 12:322–326

    CAS  Google Scholar 

  • Fukui T, Doi Y (1998) Effecient production of polyhydroxyalkanoates from plant oils by Alcaligenes eutrophus and its recombinant strain. Appl Microbiol Biotechnol 49:333–336

    Article  CAS  Google Scholar 

  • He W, Tian W, Zhang G, Chen G-Q, Zhang Z (1998) Production of novel polyhydroxyalkanoates by Pseudomonas stutzeri 1317 from glucose and soybean oil. FEMS Microbiol Lett 169:45–49

    Article  CAS  Google Scholar 

  • Kahar P, Tsuge T, Taguchi K, Doi Y (2004) High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym Degrad Stab 83:79–86

    Article  CAS  Google Scholar 

  • Koller M, Bona R, Braunegg G, Hermann C, Horvat P, Kroutil M, Martinz J, Neto J, Pereira L, Varila P (2005) Production of polyhydroxyalkanoates from agricultural waste and surplus materials. Biomacromolecules 6:561–565

    Article  CAS  Google Scholar 

  • Lee SY, Choi J-I (1998) Effect of fermentation performance on the economics of poly(3-hydroxybutyrate) production by Alcaligenes latus. Polym Degrad Stab 59:387–393

    Article  CAS  Google Scholar 

  • Lee SY, Middelberg APJ, Lee YK (1997) Poly(3-hydroxybutyrate) production from whey using recombinant Escherichia coli. Biotechnol Lett 19:1033–1035

    Article  CAS  Google Scholar 

  • Lenz RW, Marchessault RH (2005) Bacterial polyesters: Biosynthesis, biodegradable plastics and biotechnology. Biomacromolecules 6:1–8

    Article  CAS  Google Scholar 

  • Loo C-Y, Lee W-H, Tsuge T, Doi Y, Sudesh K (2005) Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from palm oil products in a Wautersia eutropha mutant. Biotechnol Lett 27:1405–1410

    Article  CAS  Google Scholar 

  • Luengo JM, García B, Sandoval A, Naharro G, Olivera ER (2003) Bioplastics from microorganisms. Curr Opinion Microbiol 6:251–260

    Article  CAS  Google Scholar 

  • Lynd LR, Wyman CE, Gerngross TU (1999) Biocommodity engineering. Biotechnol Prog 15:777–793

    Article  CAS  Google Scholar 

  • Marangoni C, Furigo A Jr, de Aragão GMF (2002) Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha in whey and inverted sugar with propionic acid feeding. Proc Biochem 38:137–141

    Article  CAS  Google Scholar 

  • Mercan N, Beyatli Y (2005) Production of poly-β-hydroxybutyrate (PHB) by Rhizobium meliloti, R. viciae and Bradyrhizobium japonicum with different carbon and nitrogen sources, and inexpensive substrates. Zuckerindustrie 130:410–415

    CAS  Google Scholar 

  • Page WJ, Manchak J, Rudy B (1992) Formation of poly(hydroxybutyrate-co-hydroxyvalerate) by Azotobacter vinelandii UWD. Appl Environ Microbiol 58:2866–2873

    Article  CAS  Google Scholar 

  • Park SJ, Park JP, Lee SY (2002) Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter. Biotechnol Lett 24:185–189

    Article  CAS  Google Scholar 

  • Povolo S, Casella S (2003) Bacterial production of PHA from lactose and cheese whey permeate. Macromol Symp 197:1–9

    Article  CAS  Google Scholar 

  • Shimamura E, Kasuya K, Kobayashi G, Shiotani T, Shima Y, Doi Y (1994) Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 27:878–880

    Article  CAS  Google Scholar 

  • Solaiman DKY, Ashby RD (2005) Genetic characterization of the poly(hydroxyalkanoate) synthases of various Pseudomonas oleovorans strains. Curr Microbiol 50:329–333

    Article  CAS  Google Scholar 

  • Solaiman DKY, Ashby RD, Foglia TA (2001) Production of polyhydroxyalkanoates from intact triacylglycerols by genetically engineered Pseudomonas. Appl Microbiol Biotechnol 56:664–669

    Article  CAS  Google Scholar 

  • Solaiman DKY, Ashby RD, Foglia TA (2002) Physiological characterization and genetic engineering of Pseudomonas corrugata for medium-chain-length polyhydroxyalkanoates synthesis from triacylglycerols. Curr Microbiol 44:189–195

    Article  CAS  Google Scholar 

  • Solaiman DKY, Ashby RD, Hotchkiss AT Jr, Foglia TA (2006) Biosynthesis of medium-chain-length poly(hydroxyalkanoates) from soy molasses. Biotechnol Lett 28:157–162

    Article  CAS  Google Scholar 

  • Steinbüchel A, Lütke-Eversloh T (2003) Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 16:81–96

    Article  Google Scholar 

  • Yellore V, Desai A (1998) Production of poly-3-hydroxybutyrate from lactose and whey by Methylobacterium sp. ZP24. Lett Appl Microbiol 25:391–394

    Article  Google Scholar 

  • Yilmaz M, Beyatli Y (2005) Poly-β-hydroxybutyrate (PHB) production by a Bacillus cereus M5 strain in sugarbeet molasses. Zuckerindustrie 130:109–112

    CAS  Google Scholar 

  • Young FK, Kastner JR, May SW (1994) Microbial production of poly-β-hydroxybutyric acid from D-xylose and lactose by Pseudomonas cepacia. Appl Environ Microbiol 60:4195–4198

    Article  CAS  Google Scholar 

  • Wu Q, Huang HH, Hu GH, Chen JC, Ho KP, Chen GQ (2001) Constitutive production of poly-3-hydroxybutyrate by strain of Bacillus aureus JMa5 cultivated in molasses media. Antonie van Leeuwenhoek 80:111–118

    Article  CAS  Google Scholar 

  • Zinn M, Witholt B, Egli T (2001) Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv Drug Deliv Rev 53:5–21

    Article  CAS  Google Scholar 

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Correspondence to Daniel K. Y. Solaiman.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Solaiman, D.K.Y., Ashby, R.D., Foglia, T.A. et al. Conversion of agricultural feedstock and coproducts into poly(hydroxyalkanoates). Appl Microbiol Biotechnol 71, 783–789 (2006). https://doi.org/10.1007/s00253-006-0451-1

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  • DOI: https://doi.org/10.1007/s00253-006-0451-1

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