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Temperature has reciprocal effects on colanic acid and polysialic acid biosynthesis in E. coli K92

  • Applied Microbial and Cell Physiology
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Abstract

Escherichia coli K92 is an opportunistic pathogen bacterium able to produce polysialic acid (PA) capsules when grows at 37°C. PA polysaccharides are cell-associated homopolymers tailored from acid sialic monomers that function as virulence factors in different neuroinvasive diseases caused by certain Enterobacteriaceae. Conversely, when grows at 19°C (restrictive conditions), PA synthesis was negligible, whereas in such condition, a slimy substance started to be accumulated in the culture broths. Analysis by uronic acids colorimetric determinations, gas chromatography–mass spectrometry, and Fourier transform infrared spectroscopy allowed the isolation and identification of mucoid substance as colanic acid (CA). CA is a heteropolymer containing glucose, galactose, fucose, and glucuronic acid as monomers which seems to be involved in the protection of this bacterium against environment assaults. The study of physicochemical conditions required for CA synthesis revealed that in E. coli K92, nutrient (carbon and nitrogen sources) modulates CA production, reaching the maximal values when glucose and proline were as carbon and nitrogen sources, respectively. Furthermore, we have found that E. coli K92 is able to produce CA at all temperatures tested (from 42°C to 15°C), whereas PA synthesis only occurred when bacteria were cultured at temperatures higher than 25°C. Additionally, genetic engineering approaches revealed that the CA cluster including several genes required for synthesis was placed into a DNA fragment of 100 kb using polymerase chain reaction methodology.

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Acknowledgments

We thank Prof. Dr. José María Luengo for the critical revision of the manuscript. N. Navasa and M. Arcos were recipients of fellowships from University of León (N.N.) and Ministerio de Educación y Ciencia (M.A.). This work was supported by grants from the Dirección General de Investigación (BMC2003-03575 and AGL 2007-62428) and the Junta de Castilla y León (JCyL LE44/04 and LE032A08).

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Authors and Affiliations

  1. Department of Molecular Biology, University of León, Campus de Vegazana, 24074, León, Spain

    Nicolás Navasa, Leandro Rodríguez-Aparicio, Honorina Martínez-Blanco, Mario Arcos & Miguel Ángel Ferrero

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  1. Nicolás Navasa
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  2. Leandro Rodríguez-Aparicio
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  3. Honorina Martínez-Blanco
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  4. Mario Arcos
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  5. Miguel Ángel Ferrero
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Correspondence to Leandro Rodríguez-Aparicio or Miguel Ángel Ferrero.

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Navasa, N., Rodríguez-Aparicio, L., Martínez-Blanco, H. et al. Temperature has reciprocal effects on colanic acid and polysialic acid biosynthesis in E. coli K92. Appl Microbiol Biotechnol 82, 721–729 (2009). https://doi.org/10.1007/s00253-008-1840-4

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  • DOI: https://doi.org/10.1007/s00253-008-1840-4

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