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Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens

  • Applied Microbial and Cell Physiology
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Abstract

Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new α-tetralone derivative, (3S)-3,6,7-trihydroxy-α-tetralone (1), together with cercosporamide (2), β-sitosterol (3), and trichodermin (4). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1, 2, and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi (Fusarium oxysporium, Rhizoctonia solani, Colletotrichum gloeosporioides, and Magnaporthe oryzae) and two plant pathogenic bacteria (Xanthomonas campestris and Xanthomonas oryzae), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC50 values of 413.22 and 48.5 μg/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC50 values of 9.3 ± 2.8, 27.87 ± 1.78, 48.79 ± 2.56, 37.57 ± 1.65, 27.83 ± 0.48, and 30.37 ± 0.28 μM, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (30600002 and 30970097) and Special Fund for Agro-scientific Research in the Public Interest (200903052) to Chu-Long Zhang, Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0943) to Fu-Cheng Lin, and Young Engagement Foundation of Hangzhou Normal University (2010QN21) to Li-Wei Wang.

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Li-Wei Wang, Bai-Ge Xu, Jia-Ying Wang, Zhen-Zhu Su, Fu-Cheng Lin & Chu-Long Zhang

  2. Department of Pharmaceutical Science, College of Medicine and Public Health Management, Hangzhou Normal University, Hangzhou, 310036, China

    Li-Wei Wang

  3. Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt9/166-5, 1060, Vienna, Austria

    Christian P. Kubicek

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  1. Li-Wei Wang
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  2. Bai-Ge Xu
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  3. Jia-Ying Wang
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Correspondence to Chu-Long Zhang.

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NMR data of known compounds (PDF 98 kb)

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Wang, LW., Xu, BG., Wang, JY. et al. Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens . Appl Microbiol Biotechnol 93, 1231–1239 (2012). https://doi.org/10.1007/s00253-011-3472-3

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  • DOI: https://doi.org/10.1007/s00253-011-3472-3

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