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Cytotoxic metabolites from Perenniporia tephropora, an endophytic fungus from Taxus chinensis var. mairei

  • Applied microbial and cell physiology
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Abstract

Based on bioactivity-oriented isolation, the EtOAc extract of a culture broth of the endophytic fungus Perenniporia tephropora Z41 from Taxus chinensis var. mairei, with strong anti-Pyricularia oryzae activity, afforded a new sesquiterpenoid, perenniporin A (1), together with three known compounds, ergosterol (2), rel-(+)-(2aR,5R,5aR,8S,8aS,8bR)-decahydro-2,2,5,8-tetramethyl-2H-naphtho[1,8-bc]genfuran-5-ol (3), and albicanol (4). Their structures were elucidated by means of spectroscopic methods. All the isolated compounds and the EtOAc extract of P. tephropora Z41 (EPT) were evaluated for their cytotoxic activity against three human cancer cell lines (HeLa, SMMC-7721, and PANC-1). EPT demonstrated significant cytotoxicity with IC50 values ranging from 2 to 15 μg/mL. Compound 2 was the most cytotoxic constituent against the tested cell lines with IC50 values of 1.16, 11.63, and 11.80 μg/mL, respectively, while compounds 1, 3, and 4 exhibited moderate cytotoxicity with IC50 values ranging from 6 to 58 μg/mL. We conclude that the endophytic fungus P. tephropora is a promising source of novel and cytotoxic metabolites.

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, People’s Republic of China

    Ling-Shang Wu, Chang-Ling Hu, Ting Han, Cheng-Jian Zheng, Xue-Qin Ma & Lu-Ping Qin

  2. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Huatuo Road, Fuzhou, 350108, People’s Republic of China

    Chang-Ling Hu

  3. Department of Pharmaceutical Analysis, School of Pharmacy, NingXia Medical University, 1160 Shenli Street, Yinchuan, 750004, People’s Republic of China

    Xue-Qin Ma

  4. Faculty of Science, School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, England, UK

    Khalid Rahman

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  1. Ling-Shang Wu
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  2. Chang-Ling Hu
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Correspondence to Cheng-Jian Zheng or Lu-Ping Qin.

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Ling-Shang Wu and Chang-Ling Hu contributed equally to this work.

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Wu, LS., Hu, CL., Han, T. et al. Cytotoxic metabolites from Perenniporia tephropora, an endophytic fungus from Taxus chinensis var. mairei . Appl Microbiol Biotechnol 97, 305–315 (2013). https://doi.org/10.1007/s00253-012-4189-7

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