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The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana

  • Bioenergy and biofuels
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Abstract

The effects of iron on the growth, lipid accumulation, and gene expression profiles of the limnetic Chlorella sorokiniana CCTCC M209220 under photoautotrophy were investigated. The addition of iron up to 10−5 mol l-l increased final cell densities by nearly 2-fold at 2.3 × 107 cells/ml, growth rate by 2-fold, and the length of the exponential phase by 5 days as compared to unsupplemented controls while 10−3 mol l−1 iron was toxic. The lipid content increased from 12 % for unsupplemented cultures to 33 % at 10−4 mol l−1 iron while the highest overall lipid yield reached 179 mg l−1. A genefishing and qPCR comparison between the C. sorokiniana at low and high iron levels indicated increases in the expression of several genes, including carbonic anhydrase involved in microalgal cell growth, as well as acc1 and choline transporter related to lipid synthesis. This study provides insights into changes in gene expression and metabolism that accompany iron supplementation to Chlorella as well as potential metabolic engineering targets for improving growth and lipid synthesis in microalgae.

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Acknowledgments

This work was supported by the Chinese Natural Science Foundation For Distinguished Group (No. 50621063), Graduate Innovation Project of Central South University (No. 2010bsxt05), and NSF EFRI Grant (No. 1332344).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Minxi Wan

  2. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, People’s Republic of China

    Minxi Wan, Xuejie Jin, Jinlan Xia & Zhenyuan Nie

  3. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, MD222, Baltimore, MD, 21218, USA

    Minxi Wan, Julian N. Rosenberg, Geng Yu & Michael J. Betenbaugh

  4. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA

    George A. Oyler

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  1. Minxi Wan
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  2. Xuejie Jin
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  3. Jinlan Xia
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  4. Julian N. Rosenberg
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Correspondence to Jinlan Xia or Michael J. Betenbaugh.

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Wan, M., Jin, X., Xia, J. et al. The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana . Appl Microbiol Biotechnol 98, 9473–9481 (2014). https://doi.org/10.1007/s00253-014-6088-6

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  • DOI: https://doi.org/10.1007/s00253-014-6088-6

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